Construct: ORF TRCN0000472027
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001846.1_s317c1
- Derived from:
- ccsbBroadEn_08858
- DNA Barcode:
- GAAAGTGATAGAGCAAGGCCTCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TEKT3 (64518)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472027
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64518 | TEKT3 | tektin 3 | NM_031898.3 | 99.7% | 99.7% | 802G>A;1017A>G;1362A>C |
| 2 | human | 64518 | TEKT3 | tektin 3 | XM_011523988.2 | 99.7% | 99.7% | 802G>A;1017A>G;1362A>C |
| 3 | human | 64518 | TEKT3 | tektin 3 | XM_011523989.1 | 99.7% | 99.7% | 802G>A;1017A>G;1362A>C |
| 4 | human | 64518 | TEKT3 | tektin 3 | XM_017024954.1 | 99.7% | 99.7% | 802G>A;1017A>G;1362A>C |
| 5 | human | 64518 | TEKT3 | tektin 3 | XM_011523990.2 | 65.9% | 65.9% | (many diffs) |
| 6 | human | 64518 | TEKT3 | tektin 3 | XM_017024955.1 | 65.9% | 65.9% | (many diffs) |
| 7 | human | 64518 | TEKT3 | tektin 3 | XM_017024956.1 | 49.1% | 45.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1536
- ORF length:
- 1470
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga acgtgtaggt tgtactttaa cgacaactta cgcccaccct agaccaacac 121 caaccaactt tctaccagcc atcagtacca tggcctcaag ctacagggac cgctttcccc 181 actccaattt gacccatagc ctgagccttc cttggagacc cagcacatac tacaaagtcg 241 cctccaattc cccaagcgtg gccccgtact gcaccagatc acagagggtg tccgagaata 301 ccatgcttcc ctttgtttcc aacagaacca ctttcttcac aagatacaca ccggatgact 361 ggtacaggtc caatttaacc aactatcaag agtccaacac ttcccgacat aattcggaga 421 aactaagagt ggatacatct cgcctgattc aagacaaata tcaacaaaca agaaaaactc 481 aggcagacac aacccaaaat ctgggagaac gtgtcaatga catagggttt tggaaatctg 541 aaatcattca tgagttggat gaaatgattg gagagacaaa tgcacttact gatgtgaaga 601 aaagactgga gcgggctttg atggagactg aagcccctct tcaggtagcc cgagaatgtc 661 tatttcatcg agaaaagaga atgggaatcg acctagttca cgatgaagtt gaagcacaac 721 tgctgacgga agttgatact attctgtgtt gtcaagaaag aatgaagcta catttggata 781 aggctattgc ccaacttgca gccaacagag cgtcccagca tgagctggaa aaggacctga 841 gtgacaaaca gacggcttac cggatcaacg acaaatgcca ccacctgcgc aacacatcag 901 acggtgtcgg ctacttccgc ggagtggaga gggtcgatgc aactgtctca gtgcctgagt 961 cctgggccaa atttacagat gacaatattc tccgctccca gagtgaacgg gcagcttccg 1021 ctaagctaag agacgacatt gaaaacctct tGGTTGTGAC TGCCAATGAG ATGTGGAATC 1081 AGTTCAACAA AGTGAACTTG TCTTTCACCA ATCGCATTGC TGAGACTGCA GATGCTAAGA 1141 ATAAGATTCA GACGCACTTA GCAAAGACCC TGCAGGAGAT TTTCCAGACT GAAATGACCA 1201 TAGAATCCAT CAAGAAGGCC ATCAAGGACA AGACTGCCTT CCTGAAGGTG GCTCAGACCA 1261 GACTGGATGA GCGCACAAGA CGGCCGAACA TTGAGTTGTG CCGAGACATG GCTCAGCTAC 1321 GCCTTGTTAA CGAGGTACAC GAGGTTGACG ACACCATCCA GACCCTGCAG CAGCGCCTGA 1381 GGGATGCAGA GGACACCCTG CAGTCGCTGG TCCACATCAA AGCCACCCTC GAGTATGACC 1441 TGGCTGTCAA AGCCAATTCC CTGTACATCG ACCAGGAAAA ATGCATGAGC ATGCGCAAGA 1501 GCTACCCCAA CACCCTCCGG CTGGTCGGCT TCTGCTACCC AACTTTCTTG TACAAAGTGG 1561 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1621 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1681 AGAAAGTGAT AGAGCAAGGC CTCGGACGCG TTAAGTCgac aatcaacctc tggattacaa 1741 aatttgtgaa agatt