Construct: ORF TRCN0000472055
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002099.1_s317c1
- Derived from:
- ccsbBroadEn_15713
- DNA Barcode:
- ACAGGACGCTACATGTCGTCCACG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TADA3 (10474)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472055
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10474 | TADA3 | transcriptional adaptor 3 | NM_133480.2 | 100% | 100% | |
| 2 | human | 10474 | TADA3 | transcriptional adaptor 3 | NM_001278270.1 | 85.4% | 85.4% | 1108_1296del |
| 3 | human | 10474 | TADA3 | transcriptional adaptor 3 | NM_006354.3 | 85.4% | 85.4% | 1108_1296del |
| 4 | mouse | 101206 | Tada3 | transcriptional adaptor 3 | NM_133932.2 | 77.1% | 84.7% | (many diffs) |
| 5 | mouse | 101206 | Tada3 | transcriptional adaptor 3 | XM_006505238.3 | 75.7% | 83.1% | (many diffs) |
| 6 | mouse | 101206 | Tada3 | transcriptional adaptor 3 | XM_006505239.3 | 75.7% | 83.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1173
- ORF length:
- 1107
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgagttgaaa gactgcccct tgcagttcca cgacttcaag tctgtggatc 121 acctgaaggt ctgtccccgc tacacggcag tgctggcacg ctctgaggat gatggcatcg 181 gcatcgagga gctggacacc ctgcagctgg agctggagac cctgctgtct tctgccagcc 241 ggcgcctgcg tgtgcttgag gccgaaaccc agatcctcac cgactggcag gataagaaag 301 gtgacagacg attcctgaag ctgggtcgag accatgaact tggagctccc cccaaacatg 361 ggaagcccaa gaagcagaaa ctggaaggga aggcaggaca tgggccgggc cctggcccag 421 gacggcccaa atccaaaaac cttcagccca agatccagga atatgaattc actgatgacc 481 ctatcgacgt gccacggatc cccaaaaatg atgcccccaa caggttctgg gcttcagtgg 541 agccctactg tgctgacatc accagcgagg aggtccgcac acttgaggag ttactgaagc 601 ccccagaaga tgaggctgag cattacaaga tcccacccct ggggaagcac tactcccagc 661 gctgggccca ggaggacctg ctggaggagc agaaggatgG GGCCCGGGCA GCGGCTGTGG 721 CTGACAAGAA GAAAGGCCTC ATGGGGCCAC TGACCGAACT GGACACTAAA GATGTGGATG 781 CCCTGCTGAA GAAGTCTGAG GCCCAGCATG AACAGCCGGA AGATGGATGC CCCTTTGGTG 841 CCCTGACGCA GCGCCTCCTG CAGGCCCTGG TGGAGGAAAA TATTATTTCC CCTATGGAGG 901 ATTCTCCTAT TCCTGACATG TCTGGGAAAG AATCAGGGGC TGACGGGGCA AGCACCTCCC 961 CTCGCAATCA GAACAAGCCC TTCAGTGTGC CGCATACTAA GTCCCTGGAG AGCCGCATCA 1021 AGGAGGAGCT AATTGCCCAG GGCCTTTTGG AGTCTGAGGA CCGCCCCGCA GAGGACTCCG 1081 AGGATGAGGT CCTTGCTGAG CTTCGCAAAC GGCAGGCTGA GCTGAAGGCA CTTAGTGCCC 1141 ACAACCGCAC CAAGAAGCAC GACCTGCTGA GGTGCCCCAA CTTTCTTGTA CAAAGTGGTT 1201 GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT 1261 AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA 1321 CAGGACGCTA CATGTCGTCC ACGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa 1381 tttgtgaaag att