Construct: ORF TRCN0000472064
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009171.1_s317c1
- Derived from:
- ccsbBroadEn_09649
- DNA Barcode:
- CCGCACGATGCGTTCACTTAATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLC3 (147700)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472064
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 147700 | KLC3 | kinesin light chain 3 | NM_177417.3 | 99.9% | 99.8% | 94C>A |
| 2 | human | 147700 | KLC3 | kinesin light chain 3 | XM_024451371.1 | 90.7% | 90.6% | 1_153del;247C>A |
| 3 | human | 147700 | KLC3 | kinesin light chain 3 | XM_024451370.1 | 77.2% | 73% | 94C>A;1441_1708del;1781_1956del |
| 4 | human | 147700 | KLC3 | kinesin light chain 3 | XM_024451369.1 | 71.6% | 67.8% | (many diffs) |
| 5 | mouse | 232943 | Klc3 | kinesin light chain 3 | NM_001286038.1 | 85.3% | 89.9% | (many diffs) |
| 6 | mouse | 232943 | Klc3 | kinesin light chain 3 | NM_001286039.1 | 85.3% | 89.9% | (many diffs) |
| 7 | mouse | 232943 | Klc3 | kinesin light chain 3 | NM_146182.4 | 85.3% | 89.9% | (many diffs) |
| 8 | mouse | 232943 | Klc3 | kinesin light chain 3 | XM_017322154.1 | 77.6% | 80.8% | (many diffs) |
| 9 | mouse | 232943 | Klc3 | kinesin light chain 3 | XM_017322153.1 | 76.9% | 80.1% | (many diffs) |
| 10 | mouse | 232943 | Klc3 | kinesin light chain 3 | XM_006539826.3 | 67.6% | 71.2% | (many diffs) |
| 11 | mouse | 232943 | Klc3 | kinesin light chain 3 | XM_006539823.3 | 62.7% | 65.3% | (many diffs) |
| 12 | mouse | 232943 | Klc3 | kinesin light chain 3 | XM_017322152.1 | 60% | 60.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1581
- ORF length:
- 1512
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctgtgcag gtagcggctc ctggaagtgc agggctgggc ccagagcgcc 121 tgagccctga ggagctggtg cggcagacgc ggcaagtggt caaggggctg gaggcgctgc 181 gggcagagca ccatggcctg gctgggcacc tggcggaggc cctggcggga cagggcccgg 241 cagccggctt ggagatgctg gaggaaaagc agcaggtggt gagccactcg ctggaggcca 301 tcgagctggg gctgggcgag gcccaggtgc tgctggccct gtcggcacat gtgggtgcac 361 tggaggcaga gaagcagcgg ctgcgctcgc aggcccggcg gctggcccag gagaacgtgt 421 ggctgcggga ggaactggag gagacgcagc ggcggcttcg ggccagcgag gagtccgtgg 481 cccagctgga ggaggagaag cgccacctgg agttcctggg gcagctgcga cagtacgacc 541 caccggcgga gagccagcag tctgagtccc cgcctcgccg agacagcctg gcctccctgt 601 tccccagcga ggaggaggag aggaaaggtc ctgaggccgc aggagcagca gctgctcagc 661 agggtggcta tgagatccct gcccgccttc ggaccctgca taacctcgtg atccagtacg 721 cggggcaggg ccgctatgag gtggcggtgc ctctgtgccg ccaggccttg gaggacctgg 781 agcgcagctc gggccactgc caccctgacg tggccaccat gctcaacatc ctggcgctgg 841 tgtaccggga ccagaacaag tacaaagaag ccacagacct tctccatgat gccctgcaga 901 tccgggagca gacgctgggc cctgagcacc ccgcggtggc cgccacgctc aacaacttgg 961 ctgtcctcta tgggaagcgt gggcgttacc gggaggcaga gcccctgtgc cagcgcgctt 1021 tggagatccg agagaaggtc ctgggtgctg accacccaga tgtggccaag cagctcaaca 1081 acctggccct gctgtgccag aaccagggca agtttgagga cgtggagcgg cactatgccc 1141 gggccctgag catctatgag gcactgggcg ggccccatga ccccaacgtg gccaagacca 1201 agaacaacct ggccTCAGCC TACCTGAAAC AGAACAAGTA TCAACAAGCG GAAGAGCTGT 1261 ACAAAGAAAT CCTCCACAAG GAGGACCTAC CCGCCCCTCT CGGTGCCCCC AACACAGGCA 1321 CAGCTGGTGA CGCAGAACAG GCCCTTCGCC GCAGCAGCTC ACTCTCCAAG ATCCGTGAGT 1381 CTATCAGGCG AGGAAGTGAG AAGCTGGTCT CCCGGCTCCG AGGCGAGGCG GCGGCAGGAG 1441 CAGCCGGAAT GAAGAGAGCC ATGTCACTCA ACACACTGAA CGTGGATGCT CCAAGGGCTC 1501 CTGGGACTCA GTTTCCCAGC TGGCACCTGG ACAAGGCCCC TCGGACCCTC AGCGCCAGCA 1561 CCCAGGACCT GAGCCCCCAC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1621 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1681 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCGC ACGATGCGTT 1741 CACTTAATAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt