Construct: ORF TRCN0000472078

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004120.1_s317c1
Derived from:
ccsbBroadEn_02989
DNA Barcode:
CAGTGCCTTAAGAAGGGCCGATCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
VPS41 (27072)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472078
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27072 VPS41 VPS41 subunit of HOPS complex NM_014396.4 100% 100%
2 human 27072 VPS41 VPS41 subunit of HOPS complex NM_080631.3 97% 97% 246_247ins75
3 human 27072 VPS41 VPS41 subunit of HOPS complex XM_017011988.1 54.4% 54% (many diffs)
4 human 27072 VPS41 VPS41 subunit of HOPS complex XR_926930.3 43% 1_26del;2430_2523del;2683_5956del
5 mouse 218035 Vps41 VPS41 HOPS complex subunit NM_172120.4 90.8% 97.1% (many diffs)
6 mouse 218035 Vps41 VPS41 HOPS complex subunit XM_006516641.2 73.7% 79.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2628
ORF length:
2562
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggaagcagag gagcaggaaa ctgggtccct tgaagaatct acagatgagt
121ctgaggaaga agagagcgaa gaggaaccca agctgaagta tgaaaggctt tccaatgggg
181taactgaaat acttcagaag gatgcagcta gctgcatgac agtccatgac aagtttttgg
241cattgggcac acattatggc aaggtttatt tacttgatgt ccaggggaac atcactcaga
301agtttgatgt aagtcctgtg aagataaatc agattagctt ggatgaaagt ggagagcaca
361tgggtgtgtg ttcagaggat ggcaaggtgc aggtatttgg actgtattct ggagaagaat
421ttcacgagac ttttgactgt cccattaaaa ttattgctgt gcacccacat ttcgtgagat
481ccagttgcaa gcagtttgtg accggaggga agaagctgct actgtttgaa cggtcttgga
541tgaacagatg gaagtctgct gttctgcatg aaggggaagg gaacataagg agtgtgaagt
601ggagaggcca tctgattgct tgggccaata atatgggtgt gaagattttt gacatcatct
661caaagcaaag aatcaccaat gtgccccggg atgatataag tcttcgccca gacatgtatc
721cctgcagcct ctgctggaag gacaatgtga cactgattat tggctggggg acttctgtca
781aggtgtgctc agtgaaggaa cggcatgcca gtgaaatgag ggatttgcca agtcgatatg
841ttgaaatagt gtctcagttt gaaactgaat tctacatcag tggacttgca cctctctgtg
901atcagcttgt tgtactttcg tatgtaaagg agatttcaga aaaaacggaa agagaatact
961gtgccaggcc tagactggac atcatccagc cactttctga gacttgtgaa gagatctctt
1021ctgatgcttt gacagtcaga ggctttcagg agaatgaatg tagagattat catttagaat
1081actctgaagg ggaatcactt ttttacatcg tgagtccgag agatgttgta gtggccaagg
1141aacgagacca agatgatcac attgactggc tccttgaaaa gaagaaatat gaagaagcat
1201tgatggcagc tgaaattagc caaaaaaata ttaaaagaca taagattctg gatattggct
1261tggcatatat aaatcacctg gtggagagag gagactatga catagcagca cgcaaatgcc
1321agaaaattct tgggaaaaat gcagcactct gggaatatga agtttataaa tttaaagaaa
1381ttggacagct taaggctatt agtccttatt tgccaagagg tgatccagtt ctgaaaccac
1441tcatctatga aatgatctta catgaatttt tggagagtga ttatgagggt tttgccacat
1501tgatccgaga atggcctgga gatctgtata ataattcagt catagttcaa gcagttcggg
1561atcatttgaa gaaagatagt cagaacaaga ctttacttaa aaccctggca gaattgtaca
1621cctatgacaa gaactatggc aatgctctgg aaatatactt aacattaaga cataaagacg
1681tttttcagtt gatccacaag cataatcttt tcagttctat caaggataaa attgttttat
1741taatggattt tgattcagag aaagctgttg acatgctttt ggacaatgaa gataaaattt
1801caattaaaaa ggtagtggaa gaattggaag acagaccaga gctacagcat gtgtatttgc
1861ataagctttt caagagagac caccataagg ggcagcgtta ccatgaaaaa cagatcagtc
1921tttatgctga atatgatcga ccaaacttac ttccctttct ccgagacagt acccattgcc
1981cacttgaaaa ggctcttgag atctgtcaac agagaaactt tgtagaagag acagtttatc
2041ttctgagccg aatgggtaat agccgaagtg ccctgaagat gattatggag gaattacatg
2101atgttgataa agcaatcgaa tttgccaagg agcaagatga tggagagctg tgggaagatt
2161tgattttata ttccattgaC AAACCACCAT TTATTACTGG CTTGTTAAAC AACATTGGCA
2221CACATGTTGA CCCAATTCTA CTGATTCACC GTATTAAGGA AGGAATGGAG ATCCCCAATT
2281TGAGAGATTC CTTGGTTAAA ATTCTGCAAG ACTACAATTT GCAAATTCTG CTTCGTGAAG
2341GCTGCAAGAA GATTCTCGTA GCTGACTCTT TGTCCTTACT GAAGAAAATG CACCGAACTC
2401AAATGAAAGG TGTTCTTGTT GATGAGGAGA ACATCTGTGA GTCGTGCCTT TCCCCTATTC
2461TTCCATCAGA TGCAGCTAAG CCCTTCAGCG TGGTGGTCTT CCATTGCCGG CACATGTTCC
2521ACAAGGAGTG CCTGCCCATG CCCAGCATGA ACTCTGCTGC ACAGTTCTGC AACATCTGCA
2581GTGCTAAGAA CCGTGGACCA GGAAGTGCAA TTTTGGAGAT GAAAAAATAC CCAACTTTCT
2641TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
2701CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
2761GTGGAAAGGA CGACAGTGCC TTAAGAAGGG CCGATCTACG CGTTAAGTCg acaatcaacc
2821tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)