Construct: ORF TRCN0000472103
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017723.1_s317c1
- Derived from:
- ccsbBroadEn_00173
- DNA Barcode:
- TGTGCGGCCGCCACCACGTGCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BRCA1 (672)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472103
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 672 | BRCA1 | BRCA1 DNA repair associated | NM_007299.4 | 100% | 100% | |
| 2 | human | 672 | BRCA1 | BRCA1 DNA repair associated | NM_007298.3 | 92% | 87.6% | 2021_2094del;2172_2277del |
| 3 | human | 672 | BRCA1 | BRCA1 DNA repair associated | NM_007294.4 | 37.5% | 36% | (many diffs) |
| 4 | human | 672 | BRCA1 | BRCA1 DNA repair associated | NM_007300.4 | 37.1% | 35.6% | (many diffs) |
| 5 | human | 672 | BRCA1 | BRCA1 DNA repair associated | NM_007297.4 | 34.9% | 33.5% | (many diffs) |
| 6 | human | 672 | BRCA1 | BRCA1 DNA repair associated | NR_027676.2 | 28.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2163
- ORF length:
- 2097
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tttatctgct cttcgcgttg aagaagtaca aaatgtcatt aatgctatgc 121 agaaaatctt agagtgtccc atctgtctgg agttgatcaa ggaacctgtc tccacaaagt 181 gtgaccacat attttgcaaa ttttgcatgc tgaaacttct caaccagaag aaagggcctt 241 cacagtgtcc tttatgtaag aatgatataa ccaaaaggag cctacaagaa agtacgagat 301 ttagtcaact tgttgaagag ctattgaaaa tcatttgtgc ttttcagctt gacacaggtt 361 tggagtatgc aaacagctat aattttgcaa aaaaggaaaa taactctcct gaacatctaa 421 aagatgaagt ttctatcatc caaagtatgg gctacagaaa ccgtgccaaa agacttctac 481 agagtgaacc cgaaaatcct tccttgcagg aaaccagtct cagtgtccaa ctctctaacc 541 ttggaactgt gagaactctg aggacaaagc agcggataca acctcaaaag acgtctgtct 601 acattgaatt gggatctgat tcttctgaag ataccgttaa taaggcaact tattgcagtg 661 tgggagatca agaattgtta caaatcaccc ctcaaggaac cagggatgaa atcagtttgg 721 attctgcaaa aaaggctgct tgtgaatttt ctgagacgga tgtaacaaat actgaacatc 781 atcaacccag taataatgat ttgaacacca ctgagaagcg tgcagctgag aggcatccag 841 aaaagtatca gggtgaagca gcatctgggt gtgagagtga aacaagcgtc tctgaagact 901 gctcagggct atcctctcag agtgacattt taaccactca gcagagggat accatgcaac 961 ataacctgat aaagctccag caggaaatgg ctgaactaga agctgtgtta gaacagcatg 1021 ggagccagcc ttctaacagc tacccttcca tcataagtga ctcttctgcc cttgaggacc 1081 tgcgaaatcc agaacaaagc acatcagaaa aagtattaac ttcacagaaa agtagtgaat 1141 accctataag ccagaatcca gaaggccttt ctgctgacaa gtttgaggtg tctgcagata 1201 gttctaccag taaaaataaa gaaccaggag tggaaaggtc atccccttct aaatgcccat 1261 cattagatga taggtggtac atgcacagtt gctctgggag tcttcagaat agaaactacc 1321 catctcaaga ggagctcatt aaggttgttg atgtggagga gcaacagctg gaagagtctg 1381 ggccacacga tttgacggaa acatcttact tgccaaggca agatctagag ggaacccctt 1441 acctggaatc tggaatcagc ctcttctctg atgaccctga atctgatcct tctgaagaca 1501 gagccccaga gtcagctcgt gttggcaaca taccatcttc aacctctgca ttgaaagttc 1561 cccaattgaa agttgcagaa tctgcccaga gtccagctgc tgctcatact actgatactg 1621 ctgggtataa tgcaatggaa gaaagtgtga gcagggagaa gccagaattG ACAGCTTCAA 1681 CAGAAAGGGT CAACAAAAGA ATGTCCATGG TGGTGTCTGG CCTGACCCCA GAAGAATTTA 1741 TGCTCGTGTA CAAGTTTGCC AGAAAACACC ACATCACTTT AACTAATCTA ATTACTGAAG 1801 AGACTACTCA TGTTGTTATG AAAACAGATG CTGAGTTTGT GTGTGAACGG ACACTGAAAT 1861 ATTTTCTAGG AATTGCGGGA GGAAAATGGG TAGTTAGCTA TTTCTGGGTG ACCCAGTCTA 1921 TTAAAGAAAG AAAAATGCTG AATGAGCATG ATTTTGAAGT CAGAGGAGAT GTGGTCAATG 1981 GAAGAAACCA CCAAGGTCCA AAGCGAGCAA GAGAATCCCA GGACAGAAAG ATCTTCAGGG 2041 GGCTAGAAAT CTGTTGCTAT GGGCCCTTCA CCAACATGCC CACAGGGTGT CCACCCAATT 2101 GTGGTTGTGC AGCCAGATGC CTGGACAGAG GACAATGGCT TCCATGCAAT TGGGCAGATG 2161 TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2221 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2281 GGCTTTATAT ATCTTGTGGA AAGGACGATG TGCGGCCGCC ACCACGTGCG CAACGCGTTA 2341 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt