Construct: ORF TRCN0000472105
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006036.1_s317c1
- Derived from:
- ccsbBroadEn_12753
- DNA Barcode:
- CCTAACTGAAGGGCCTCCGCGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YPEL3 (83719)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472105
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83719 | YPEL3 | yippee like 3 | NM_031477.5 | 78.1% | 78.1% | 0_1ins132 |
| 2 | human | 83719 | YPEL3 | yippee like 3 | NM_001145524.2 | 59.2% | 59.2% | 0_1ins246 |
| 3 | human | 83719 | YPEL3 | yippee like 3 | XR_950857.1 | 55% | (many diffs) | |
| 4 | human | 29799 | YPEL1 | yippee like 1 | NM_013313.5 | 47.6% | 52.2% | (many diffs) |
| 5 | mouse | 66090 | Ypel3 | yippee-like 3 (Drosophila) | XM_017312227.1 | 70.1% | 73.1% | (many diffs) |
| 6 | mouse | 66090 | Ypel3 | yippee-like 3 (Drosophila) | NM_025347.2 | 56.5% | 59.2% | (many diffs) |
| 7 | mouse | 66090 | Ypel3 | yippee-like 3 (Drosophila) | NM_026875.2 | 56.5% | 59.2% | (many diffs) |
| 8 | mouse | 77864 | Ypel2 | yippee-like 2 (Drosophila) | NM_001005341.3 | 47.7% | 52.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 669
- ORF length:
- 603
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca acctgaggcc tcctcactga gtcaaaaccg agacgcctct ggtccccgga 121 gaggctgctg gctctgggaa aggagagagc agcccagtgt gacagagcga gTCCCCACGG 181 CTCTGGGAGT GCCTCGCATG TGTGTGGCCC AGGTCCTGAC AGCCCACCTA CTCCCTCCCC 241 GGCAGGCACT GGGCTCCCTC TGCTCCCCGT GGGCCGCTCC CCGCGTGGGG CCACTGCCCC 301 CGGCCCCCGC CATGGTGCGG ATTTCAAAGC CCAAGACGTT TCAGGCCTAC TTGGATGATT 361 GTCACCGGAG GTATAGCTGT GCCCACTGCC GCGCTCACCT GGCCAACCAC GACGACCTCA 421 TCTCCAAGTC CTTCCAGGGC AGTCAGGGGC GTGCCTACCT CTTCAACTCA GTGGTGAACG 481 TGGGCTGCGG GCCAGCCGAG GAGCGGGTGC TGCTGACCGG CCTCCATGCT GTCGCCGACA 541 TCCACTGCGA GAACTGCAAG ACCACTTTGG GCTGGAAATA TGAACAGGCC TTTGAGAGCA 601 GCCAGAAGTA CAAAGAGGGG AAGTACATCA TTGAACTCAA CCACATGATC AAAGACAACG 661 GCTGGGACTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 721 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 781 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCTAAC TGAAGGGCCT CCGCGATTAC 841 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt