Construct: ORF TRCN0000472112
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002287.1_s317c1
- Derived from:
- ccsbBroadEn_04486
- DNA Barcode:
- GCCAGTCCGACTAGCATGACGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJC5B (85479)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472112
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85479 | DNAJC5B | DnaJ heat shock protein fam... | NM_001349432.1 | 100% | 100% | |
| 2 | human | 85479 | DNAJC5B | DnaJ heat shock protein fam... | NM_033105.6 | 100% | 100% | |
| 3 | human | 85479 | DNAJC5B | DnaJ heat shock protein fam... | XM_011517619.2 | 86.9% | 82.8% | (many diffs) |
| 4 | human | 85479 | DNAJC5B | DnaJ heat shock protein fam... | XM_011517620.2 | 86.9% | 85.9% | (many diffs) |
| 5 | human | 85479 | DNAJC5B | DnaJ heat shock protein fam... | XM_011517621.2 | 82.3% | 78.4% | (many diffs) |
| 6 | human | 85479 | DNAJC5B | DnaJ heat shock protein fam... | NR_146171.2 | 15.6% | (many diffs) | |
| 7 | mouse | 66326 | Dnajc5b | DnaJ heat shock protein fam... | NM_001163536.1 | 89.6% | 88.9% | (many diffs) |
| 8 | mouse | 66326 | Dnajc5b | DnaJ heat shock protein fam... | NM_001163537.1 | 89.6% | 88.9% | (many diffs) |
| 9 | mouse | 66326 | Dnajc5b | DnaJ heat shock protein fam... | NM_025489.3 | 89.6% | 88.9% | (many diffs) |
| 10 | mouse | 66326 | Dnajc5b | DnaJ heat shock protein fam... | XM_006535515.1 | 89.6% | 88.9% | (many diffs) |
| 11 | mouse | 66326 | Dnajc5b | DnaJ heat shock protein fam... | XM_006535516.1 | 89.6% | 88.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 663
- ORF length:
- 597
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc atgtaacata cctaaccaaa gacagcggac tctgtcaaca acaggagaag 121 ctctatacga aattcttggt ctgcataagg gagcatcaaa tgaagaaatt aagaaaacct 181 acagaaaatt ggcccTGAAA CACCATCCAG ACAAGAATCC AGATGATCCA GCTGCTACTG 241 AGAAGTTTAA AGAAATCAAC AACGCCCACG CAATACTTAC CGACATTTCA AAGAGAAGCA 301 TATACGACAA GTACGGATCG CTGGGACTCT ACGTGGCCGA GCAGTTTGGA GACGAAAACG 361 TTAACACCTA CTTCATGCTG TCGAGCTGGT GGGCAAAGGC CCTGTTTGTC ATCGTTGGCC 421 TCTTGACGGG CTGCTACTTT TGCTGCTGCC TGTGCTGCTG CTGCAACTGC TGCTGTGGAC 481 ACTGCCGGCC CGAGTCATCA GTGCCAGAAG AGGACTTCTA TGTGTCCCCA GAGGATCTGG 541 AGGAGCAGAT CAAGTCTGAC ATGGAAAAAG ATGTGGACTT TCCAGTTTTT CTCCAGCCTA 601 CAAATGCAAA TGAGAAAACA CAGCTAATCA AAGAAGGATC TCGAAGTTAT TGCACAGACT 661 CTTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 721 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 781 GGCTTTATAT ATCTTGTGGA AAGGACGAGC CAGTCCGACT AGCATGACGT ACACGCGTTA 841 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt