Construct: ORF TRCN0000472143
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010142.1_s317c1
- Derived from:
- ccsbBroadEn_02773
- DNA Barcode:
- CGCTTCGCCCCGGCTCTCTCGGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRAM1 (23471)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472143
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23471 | TRAM1 | translocation associated me... | NM_014294.6 | 100% | 100% | |
| 2 | human | 23471 | TRAM1 | translocation associated me... | NM_001317804.2 | 91.7% | 91.7% | 0_1ins93 |
| 3 | human | 23471 | TRAM1 | translocation associated me... | NM_001317805.2 | 77% | 77% | 0_1ins258 |
| 4 | human | 23471 | TRAM1 | translocation associated me... | XM_017013266.1 | 74.3% | 72.4% | 0_1ins166;21_22ins122 |
| 5 | human | 23471 | TRAM1 | translocation associated me... | XM_017013267.2 | 74.3% | 72.4% | 0_1ins166;21_22ins122 |
| 6 | mouse | 72265 | Tram1 | translocating chain-associa... | NM_028173.5 | 87.3% | 93% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1188
- ORF length:
- 1122
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gattcgcaag aaaagcacca agagcccccc agtgctgagc cacgaattcg 121 tcctgcagaa tcacgcggac atcgtctcct gtgtggcgat ggtcttcctg ctggggctca 181 tgtttgagat aacggcaaaa gcttctatca tttttgttac tcttcagtac aatgtcaccc 241 tcccagcaac agaagaacaa gctactgaat cagtgtccct ttattactat ggcatcaaag 301 atttggctac tgttttcttc tacatgctag tggcgataat tattcatgcc gtaattcaag 361 agtatatgtt ggataaaatt aacaggcgaa tgcacttctc caaaacaaaa cacagcaagt 421 ttaatgaatc tggtcagctt agtgcgttct acctttttgc ctgtgtttgg ggcacattca 481 ttctcatctc tgaaaactac atctcagacc caactatctt atggagggct tatccccata 541 acctgatgac atttcaaatg aagtttttct acatatcaca gctggcttac tggcttcatg 601 cttttcctga actctacttc cagaaaacca aaaaagaaga tattcctcgt cagcttgtct 661 acattggtct ttacctcttc cacattgctg gagcttacct tttgaacttg aatcatctag 721 gacttgtTCT TCTGGTGCTA CATTATTTTG TTGAATTTCT TTTCCACATT TCCCGCCTGT 781 TTTATTTTAG CAATGAAAAG TATCAGAAAG GATTTTCTCT GTGGGCAGTT CTTTTTGTTT 841 TGGGAAGACT TCTGACTTTA ATTCTTTCAG TACTGACTGT TGGTTTTGGC CTTGCAAGAG 901 CAGAAAATCA GAAGCTGGAT TTCAGTACTG GAAACTTCAA TGTGTTAGCT GTTAGAATCG 961 CTGTTCTGGC ATCCATTTGC GTTACTCAGG CATTTATGAT GTGGAAGTTC ATTAATTTTC 1021 AGCTTCGAAG GTGGAGGGAA CATTCTGCTT TTCAGGCACC AGCTGTGAAG AAGAAACCAA 1081 CAGTAACTAA AGGCAGATCT TCTAAAAAAG GAACAGAAAA TGGTGTGAAT GGAACATTAA 1141 CTTCAAATGT AGCAGACTCT CCCCGGAATA AAAAAGAGAA ATCTTCATAC CCAACTTTCT 1201 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1261 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1321 GTGGAAAGGA CGACGCTTCG CCCCGGCTCT CTCGGCTACG CGTTAAGTCg acaatcaacc 1381 tctggattac aaaatttgtg aaagatt