Construct: ORF TRCN0000472143

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010142.1_s317c1
Derived from:
ccsbBroadEn_02773
DNA Barcode:
CGCTTCGCCCCGGCTCTCTCGGCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TRAM1 (23471)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472143
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23471 TRAM1 translocation associated me... NM_014294.6 100% 100%
2 human 23471 TRAM1 translocation associated me... NM_001317804.2 91.7% 91.7% 0_1ins93
3 human 23471 TRAM1 translocation associated me... NM_001317805.2 77% 77% 0_1ins258
4 human 23471 TRAM1 translocation associated me... XM_017013266.1 74.3% 72.4% 0_1ins166;21_22ins122
5 human 23471 TRAM1 translocation associated me... XM_017013267.2 74.3% 72.4% 0_1ins166;21_22ins122
6 mouse 72265 Tram1 translocating chain-associa... NM_028173.5 87.3% 93% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1188
ORF length:
1122
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gattcgcaag aaaagcacca agagcccccc agtgctgagc cacgaattcg
121tcctgcagaa tcacgcggac atcgtctcct gtgtggcgat ggtcttcctg ctggggctca
181tgtttgagat aacggcaaaa gcttctatca tttttgttac tcttcagtac aatgtcaccc
241tcccagcaac agaagaacaa gctactgaat cagtgtccct ttattactat ggcatcaaag
301atttggctac tgttttcttc tacatgctag tggcgataat tattcatgcc gtaattcaag
361agtatatgtt ggataaaatt aacaggcgaa tgcacttctc caaaacaaaa cacagcaagt
421ttaatgaatc tggtcagctt agtgcgttct acctttttgc ctgtgtttgg ggcacattca
481ttctcatctc tgaaaactac atctcagacc caactatctt atggagggct tatccccata
541acctgatgac atttcaaatg aagtttttct acatatcaca gctggcttac tggcttcatg
601cttttcctga actctacttc cagaaaacca aaaaagaaga tattcctcgt cagcttgtct
661acattggtct ttacctcttc cacattgctg gagcttacct tttgaacttg aatcatctag
721gacttgtTCT TCTGGTGCTA CATTATTTTG TTGAATTTCT TTTCCACATT TCCCGCCTGT
781TTTATTTTAG CAATGAAAAG TATCAGAAAG GATTTTCTCT GTGGGCAGTT CTTTTTGTTT
841TGGGAAGACT TCTGACTTTA ATTCTTTCAG TACTGACTGT TGGTTTTGGC CTTGCAAGAG
901CAGAAAATCA GAAGCTGGAT TTCAGTACTG GAAACTTCAA TGTGTTAGCT GTTAGAATCG
961CTGTTCTGGC ATCCATTTGC GTTACTCAGG CATTTATGAT GTGGAAGTTC ATTAATTTTC
1021AGCTTCGAAG GTGGAGGGAA CATTCTGCTT TTCAGGCACC AGCTGTGAAG AAGAAACCAA
1081CAGTAACTAA AGGCAGATCT TCTAAAAAAG GAACAGAAAA TGGTGTGAAT GGAACATTAA
1141CTTCAAATGT AGCAGACTCT CCCCGGAATA AAAAAGAGAA ATCTTCATAC CCAACTTTCT
1201TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1261CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1321GTGGAAAGGA CGACGCTTCG CCCCGGCTCT CTCGGCTACG CGTTAAGTCg acaatcaacc
1381tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)