Transcript: Human NM_001317804.2

Homo sapiens translocation associated membrane protein 1 (TRAM1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
TRAM1 (23471)
Length:
2973
CDS:
228..1259

Additional Resources:

NCBI RefSeq record:
NM_001317804.2
NBCI Gene record:
TRAM1 (23471)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001317804.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000062939 CGATAATTATTCATGCCGTAA pLKO.1 403 CDS 100% 4.050 5.670 N TRAM1 n/a
2 TRCN0000304158 TGTTCTAAGAAGGCCATATTT pLKO_005 1625 3UTR 100% 15.000 10.500 N TRAM1 n/a
3 TRCN0000304157 TCGCTGTTCTGGCATCCATTT pLKO_005 1027 CDS 100% 10.800 7.560 N TRAM1 n/a
4 TRCN0000062941 GAAGAAACCAACAGTAACTAA pLKO.1 1139 CDS 100% 5.625 3.938 N TRAM1 n/a
5 TRCN0000300865 GAAGAAACCAACAGTAACTAA pLKO_005 1139 CDS 100% 5.625 3.938 N TRAM1 n/a
6 TRCN0000062940 ACTGGAAACTTCAATGTGTTA pLKO.1 996 CDS 100% 4.950 3.465 N TRAM1 n/a
7 TRCN0000300789 ACTGGAAACTTCAATGTGTTA pLKO_005 996 CDS 100% 4.950 3.465 N TRAM1 n/a
8 TRCN0000062938 CCTTTATTACTATGGCATCAA pLKO.1 347 CDS 100% 4.950 3.465 N TRAM1 n/a
9 TRCN0000062942 CAACTATCTTATGGAGGGCTT pLKO.1 580 CDS 100% 2.160 1.512 N TRAM1 n/a
10 TRCN0000300788 CAACTATCTTATGGAGGGCTT pLKO_005 580 CDS 100% 2.160 1.512 N TRAM1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001317804.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02773 pDONR223 100% 91.7% 91.7% None 0_1ins93 n/a
2 ccsbBroad304_02773 pLX_304 0% 91.7% 91.7% V5 0_1ins93 n/a
3 TRCN0000472143 CGCTTCGCCCCGGCTCTCTCGGCT pLX_317 41.2% 91.7% 91.7% V5 0_1ins93 n/a
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