Construct: ORF TRCN0000472170
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005453.4_s317c1
- Derived from:
- ccsbBroadEn_12670
- DNA Barcode:
- GAGAGAACCACCTCTTATGACATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP3K19 (80122)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472170
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80122 | MAP3K19 | mitogen-activated protein k... | NM_001282883.1 | 36.5% | 36.5% | 1_813del;1318_1380del |
| 2 | human | 80122 | MAP3K19 | mitogen-activated protein k... | NM_001018047.2 | 36.3% | 36.3% | 1_819del;1324_1386del |
| 3 | human | 80122 | MAP3K19 | mitogen-activated protein k... | NM_001018046.2 | 32.9% | 32.9% | 1_963del;1468_1530del |
| 4 | human | 80122 | MAP3K19 | mitogen-activated protein k... | XM_017005006.2 | 13.9% | 13.9% | 1_3051del;3556_3618del |
| 5 | human | 80122 | MAP3K19 | mitogen-activated protein k... | NM_001018044.2 | 13.8% | 13.8% | 1_3078del;3583_3645del |
| 6 | human | 80122 | MAP3K19 | mitogen-activated protein k... | XM_011511896.3 | 13.6% | 13.6% | 1_3132del;3637_3699del |
| 7 | human | 80122 | MAP3K19 | mitogen-activated protein k... | XM_011511897.3 | 13.6% | 13.6% | 1_3132del;3637_3699del |
| 8 | human | 80122 | MAP3K19 | mitogen-activated protein k... | XM_011511894.3 | 12.9% | 12.9% | 1_3333del;3838_3900del |
| 9 | human | 80122 | MAP3K19 | mitogen-activated protein k... | XM_017005004.2 | 12.9% | 12.9% | 1_3333del;3838_3900del |
| 10 | human | 80122 | MAP3K19 | mitogen-activated protein k... | XM_017005005.2 | 12.9% | 12.9% | 1_3333del;3838_3900del |
| 11 | human | 80122 | MAP3K19 | mitogen-activated protein k... | NM_025052.4 | 12.6% | 12.6% | 1_3417del;3922_3984del |
| 12 | human | 80122 | MAP3K19 | mitogen-activated protein k... | XM_011511892.1 | 12.6% | 12.6% | 1_3417del;3922_3984del |
| 13 | human | 80122 | MAP3K19 | mitogen-activated protein k... | XM_017005003.1 | 12.6% | 12.6% | 1_3417del;3922_3984del |
| 14 | human | 80122 | MAP3K19 | mitogen-activated protein k... | XM_011511891.2 | 12.4% | 12.4% | 1_3468del;3973_4035del |
| 15 | human | 80122 | MAP3K19 | mitogen-activated protein k... | XR_002959344.1 | 9.8% | 1_3556del;4061_5101del | |
| 16 | human | 80122 | MAP3K19 | mitogen-activated protein k... | XR_002959343.1 | 9.5% | 1_3740del;4245_5285del | |
| 17 | human | 80122 | MAP3K19 | mitogen-activated protein k... | XR_001738962.1 | 8.9% | 1_3708del;4087_4088ins125 | |
| 18 | mouse | 22625 | Map3k19 | mitogen-activated protein k... | NM_011737.1 | 11.2% | 12.2% | (many diffs) |
| 19 | mouse | 22625 | Map3k19 | mitogen-activated protein k... | XM_006529396.3 | 9.7% | 10.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 570
- ORF length:
- 504
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCATGGA GTTTGTTCCT GGTGGCTCAA TCTCTAGTAT TATAAACCGT TTTGGGCCAT 121 TGCCTGAGAT GGTGTTCTGT AAATATACGA AACAAATACT TCAAGGTGTT GCTTATCTCC 181 ATGAGAACTG TGTGGTACAT CGCGATATCA AAGGAAATAA TGTTATGCTC ATGCCAACTG 241 GAATAATAAA GCTGATTGAC TTTGGCTGTG CCAGGCGTTT GGCCTGGGCA GGTTTAAATG 301 GCACCCACAG TGACATGCTT AAGTCCATGC ATGGGACTCC ATATTGGATG GCCCCAGAAG 361 TCATCAATGA GTCTGGCTAT GGACGGAAAT CAGATATCTG GAGCATTGGT TGTACTGTGT 421 TTGAGATGGC TACAGGGAAG CCTCCACTGG CTTCCATGGA CAGGATGGCC GCCATGTTTT 481 ACATCGGAGC ACACCGAGGG CTGATGCCTC CTTTACCAGA CCACTTCTCA GAAAATGCAG 541 CAGACTTTGT GCGCATGTGC CTGACCAGGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 601 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 661 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAGAG 721 AACCACCTCT TATGACATCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 781 tgaaagatt