Transcript: Human NM_001018046.2

Homo sapiens mitogen-activated protein kinase kinase kinase 19 (MAP3K19), transcript variant 2, mRNA.

Source:
NCBI, updated 2018-06-30
Taxon:
Homo sapiens (human)
Gene:
MAP3K19 (80122)
Length:
1933
CDS:
32..1564

Additional Resources:

NCBI RefSeq record:
NM_001018046.2
NBCI Gene record:
MAP3K19 (80122)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001018046.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000355773 TGGCTCAATCTCTAGTATTAT pLKO_005 1012 CDS 100% 15.000 21.000 N MAP3K19 n/a
2 TRCN0000002392 TCTGGCTATGGACGGAAATCA pLKO.1 1301 CDS 100% 5.625 4.500 N MAP3K19 n/a
3 TRCN0000002394 GATGGTGTTCTGTAAATATAC pLKO.1 1057 CDS 100% 13.200 9.240 N MAP3K19 n/a
4 TRCN0000002391 AGCATTGGTTGTACTGTGTTT pLKO.1 1331 CDS 100% 4.950 3.465 N MAP3K19 n/a
5 TRCN0000002393 GAGCATTGGTTGTACTGTGTT pLKO.1 1330 CDS 100% 4.950 3.465 N MAP3K19 n/a
6 TRCN0000194794 CCAACTGATTTGATGACAGTT pLKO.1 104 CDS 100% 0.495 0.347 N MAP3K19 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001018046.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12670 pDONR223 100% 32.9% 32.9% None 1_963del;1468_1530del n/a
2 ccsbBroad304_12670 pLX_304 0% 32.9% 32.9% V5 1_963del;1468_1530del n/a
3 TRCN0000472170 GAGAGAACCACCTCTTATGACATC pLX_317 98.8% 32.9% 32.9% V5 1_963del;1468_1530del n/a
4 ccsbBroadEn_15162 pDONR223 0% 32.9% 32.9% None 1_963del;1468_1530del n/a
5 ccsbBroad304_15162 pLX_304 0% 32.9% 32.9% V5 1_963del;1468_1530del n/a
6 TRCN0000467073 TGCAGCGACGCCTCGATCAATGGT pLX_317 60.4% 32.9% 32.9% V5 1_963del;1468_1530del n/a
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