Construct: ORF TRCN0000472207
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014419.1_s317c1
- Derived from:
- ccsbBroadEn_15486
- DNA Barcode:
- TAACCCGTTACCCGTCAGACCCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCND3 (3752)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472207
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3752 | KCND3 | potassium voltage-gated cha... | NM_172198.2 | 99.9% | 100% | 1122G>T |
| 2 | human | 3752 | KCND3 | potassium voltage-gated cha... | XM_006710630.3 | 99.9% | 100% | 1122G>T |
| 3 | human | 3752 | KCND3 | potassium voltage-gated cha... | NM_004980.4 | 97% | 97% | 1122G>T;1462_1518del |
| 4 | human | 3752 | KCND3 | potassium voltage-gated cha... | XM_005270851.4 | 97% | 97% | 1122G>T;1462_1518del |
| 5 | human | 3752 | KCND3 | potassium voltage-gated cha... | XM_006710629.4 | 97% | 97% | 1122G>T;1462_1518del |
| 6 | human | 3752 | KCND3 | potassium voltage-gated cha... | XM_017001244.2 | 97% | 97% | 1122G>T;1462_1518del |
| 7 | human | 3752 | KCND3 | potassium voltage-gated cha... | XM_006710631.3 | 78.3% | 77.1% | (many diffs) |
| 8 | human | 3752 | KCND3 | potassium voltage-gated cha... | XM_011541425.3 | 61.2% | 59.4% | (many diffs) |
| 9 | human | 3752 | KCND3 | potassium voltage-gated cha... | XM_017001245.2 | 61.2% | 59.4% | (many diffs) |
| 10 | human | 3752 | KCND3 | potassium voltage-gated cha... | XM_011541426.2 | 59% | 58.4% | (many diffs) |
| 11 | human | 3752 | KCND3 | potassium voltage-gated cha... | XM_006710632.3 | 58.9% | 58.1% | (many diffs) |
| 12 | human | 3752 | KCND3 | potassium voltage-gated cha... | XM_011541427.3 | 58% | 58% | 1107_1108ins801 |
| 13 | human | 3752 | KCND3 | potassium voltage-gated cha... | XM_011541428.2 | 57.9% | 58% | 1107_1107delGins802 |
| 14 | mouse | 56543 | Kcnd3 | potassium voltage-gated cha... | NM_001039347.1 | 91.1% | 99.3% | (many diffs) |
| 15 | mouse | 56543 | Kcnd3 | potassium voltage-gated cha... | NM_019931.1 | 88.4% | 96.4% | (many diffs) |
| 16 | mouse | 56543 | Kcnd3 | potassium voltage-gated cha... | XM_006501755.3 | 88.4% | 96.4% | (many diffs) |
| 17 | mouse | 56543 | Kcnd3 | potassium voltage-gated cha... | XM_006501756.3 | 88.4% | 96.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1977
- ORF length:
- 1908
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggccgga gttgcggcct ggctgccttt tgcccgggct gcggccatcg 121 ggtggatgcc ggtggccaac tgccccatgc ccctggcccc ggccgacaag aacaagcggc 181 aggatgagct gattgtcctc aacgtgagtg ggcggaggtt ccagacctgg aggaccacgc 241 tggagcgcta cccggacacc ctgctgggca gcacggagaa ggagttcttc ttcaacgagg 301 acaccaagga gtacttcttc gaccgggacc ccgaggtgtt ccgctgcgtg ctcaacttct 361 accgcacggg gaagctgcac tacccgcgct acgagtgcat ctctgcctac gacgacgagc 421 tggccttcta cggcatcctc ccggagatca tcggggactg ctgctacgag gagtacaagg 481 accgcaagag ggagaacgcc gagcggctca tggacgacaa cgactcggag aacaaccagg 541 agtccatgcc ctcgctcagc ttccgccaga ccatgtggcg ggccttcgag aacccccaca 601 ccagcacgct ggccctggtc ttctactacg tgactggctt cttcatcgct gtctcggtca 661 tcaccaacgt ggtggagacg gtgccgtgcg gcacggtccc gggcagcaag gagctgccgt 721 gcggggagcg ctactcggtg gccttcttct gcctggacac ggcgtgcgtc atgatcttca 781 ccgtggagta cctcctgcgg ctcttcgcgg ctcccagccg ctaccgcttc atccgcagcg 841 tcatgagcat catcgacgtg gtggccatca tgccctacta catcggtctg gtcatgacca 901 acaacgagga cgtgtccggc gccttcgtca cgctccgggt cttccgcgtc ttcaggatct 961 tcaagttttc ccgccactcc cagggcctgc ggatcctggg ctacacactg aagagctgtg 1021 cctccgaact gggctttctt ctcttctccc tcaccatggc catcatcatc tttgccactg 1081 tgatgtttta tgccgagaag ggctcctcgg ccagcaagtt cacaagcatc cctgcctcgt 1141 tttggtacac cattgtcacc atgaccacac tgggatacgg agacatggtt cctaagacga 1201 ttgcagggaa gatcttcggc tccatctgct ccttgagtgg cgtcctggtc attgccctgc 1261 cagtccctgt gattgtttcc aactttagcc ggatttacca ccagaatcag agagctgata 1321 aacgcagggc acaaaagaag gcccgccttg ccaggatccg tgtggccaaa acaggcagtt 1381 cgaatgcata cctgcacagc aagcgcaacg ggctcctcaa cgaggcgctg gagctgacgg 1441 gcaccccaga agaggagcac atgggcaaga ccacctcact catcgagagc cagcatcatc 1501 acctgctgca ctgcctggaa aaaaccacta accacgagtt tattgatgag cagatgtttg 1561 agcagaactg catggagagt tcaatgcaga actacccatc cacaagaagt ccctcactgt 1621 ccagccaccc aggcctcact accacctgct gctcccgtcg tagtaagaag accacacacc 1681 tgcccaattc taacctgcca gctactcgcc TGCGCAGCAT GCAAGAGCTC AGCACGATCC 1741 ACATCCAGGG CAGTGAGCAG CCCTCCCTCA CAACCAGTCG CTCCAGCCTT AATTTGAAAG 1801 CAGACGACGG ACTGAGACCA AACTGCAAAA CATCCCAGAT CACCACAGCC ATCATCAGCA 1861 TCCCCACTCC CCCAGCGCTA ACCCCAGAGG GGGAAAGTCG GCCACCCCCT GCCAGCCCAG 1921 GCCCCAACAC GAACATTCCT TCCATAGCCA GCAATGTTGT CAAGGTCTCC GCCTTGTTGC 1981 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2041 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2101 ATATATCTTG TGGAAAGGAC GATAACCCGT TACCCGTCAG ACCCATACGC GTTAAGTCga 2161 caatcaacct ctggattaca aaatttgtga aagatt