Construct: ORF TRCN0000472207

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014419.1_s317c1
Derived from:
ccsbBroadEn_15486
DNA Barcode:
TAACCCGTTACCCGTCAGACCCAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KCND3 (3752)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472207
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3752 KCND3 potassium voltage-gated cha... NM_172198.2 99.9% 100% 1122G>T
2 human 3752 KCND3 potassium voltage-gated cha... XM_006710630.3 99.9% 100% 1122G>T
3 human 3752 KCND3 potassium voltage-gated cha... NM_004980.4 97% 97% 1122G>T;1462_1518del
4 human 3752 KCND3 potassium voltage-gated cha... XM_005270851.4 97% 97% 1122G>T;1462_1518del
5 human 3752 KCND3 potassium voltage-gated cha... XM_006710629.4 97% 97% 1122G>T;1462_1518del
6 human 3752 KCND3 potassium voltage-gated cha... XM_017001244.2 97% 97% 1122G>T;1462_1518del
7 human 3752 KCND3 potassium voltage-gated cha... XM_006710631.3 78.3% 77.1% (many diffs)
8 human 3752 KCND3 potassium voltage-gated cha... XM_011541425.3 61.2% 59.4% (many diffs)
9 human 3752 KCND3 potassium voltage-gated cha... XM_017001245.2 61.2% 59.4% (many diffs)
10 human 3752 KCND3 potassium voltage-gated cha... XM_011541426.2 59% 58.4% (many diffs)
11 human 3752 KCND3 potassium voltage-gated cha... XM_006710632.3 58.9% 58.1% (many diffs)
12 human 3752 KCND3 potassium voltage-gated cha... XM_011541427.3 58% 58% 1107_1108ins801
13 human 3752 KCND3 potassium voltage-gated cha... XM_011541428.2 57.9% 58% 1107_1107delGins802
14 mouse 56543 Kcnd3 potassium voltage-gated cha... NM_001039347.1 91.1% 99.3% (many diffs)
15 mouse 56543 Kcnd3 potassium voltage-gated cha... NM_019931.1 88.4% 96.4% (many diffs)
16 mouse 56543 Kcnd3 potassium voltage-gated cha... XM_006501755.3 88.4% 96.4% (many diffs)
17 mouse 56543 Kcnd3 potassium voltage-gated cha... XM_006501756.3 88.4% 96.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1977
ORF length:
1908
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggccgga gttgcggcct ggctgccttt tgcccgggct gcggccatcg
121ggtggatgcc ggtggccaac tgccccatgc ccctggcccc ggccgacaag aacaagcggc
181aggatgagct gattgtcctc aacgtgagtg ggcggaggtt ccagacctgg aggaccacgc
241tggagcgcta cccggacacc ctgctgggca gcacggagaa ggagttcttc ttcaacgagg
301acaccaagga gtacttcttc gaccgggacc ccgaggtgtt ccgctgcgtg ctcaacttct
361accgcacggg gaagctgcac tacccgcgct acgagtgcat ctctgcctac gacgacgagc
421tggccttcta cggcatcctc ccggagatca tcggggactg ctgctacgag gagtacaagg
481accgcaagag ggagaacgcc gagcggctca tggacgacaa cgactcggag aacaaccagg
541agtccatgcc ctcgctcagc ttccgccaga ccatgtggcg ggccttcgag aacccccaca
601ccagcacgct ggccctggtc ttctactacg tgactggctt cttcatcgct gtctcggtca
661tcaccaacgt ggtggagacg gtgccgtgcg gcacggtccc gggcagcaag gagctgccgt
721gcggggagcg ctactcggtg gccttcttct gcctggacac ggcgtgcgtc atgatcttca
781ccgtggagta cctcctgcgg ctcttcgcgg ctcccagccg ctaccgcttc atccgcagcg
841tcatgagcat catcgacgtg gtggccatca tgccctacta catcggtctg gtcatgacca
901acaacgagga cgtgtccggc gccttcgtca cgctccgggt cttccgcgtc ttcaggatct
961tcaagttttc ccgccactcc cagggcctgc ggatcctggg ctacacactg aagagctgtg
1021cctccgaact gggctttctt ctcttctccc tcaccatggc catcatcatc tttgccactg
1081tgatgtttta tgccgagaag ggctcctcgg ccagcaagtt cacaagcatc cctgcctcgt
1141tttggtacac cattgtcacc atgaccacac tgggatacgg agacatggtt cctaagacga
1201ttgcagggaa gatcttcggc tccatctgct ccttgagtgg cgtcctggtc attgccctgc
1261cagtccctgt gattgtttcc aactttagcc ggatttacca ccagaatcag agagctgata
1321aacgcagggc acaaaagaag gcccgccttg ccaggatccg tgtggccaaa acaggcagtt
1381cgaatgcata cctgcacagc aagcgcaacg ggctcctcaa cgaggcgctg gagctgacgg
1441gcaccccaga agaggagcac atgggcaaga ccacctcact catcgagagc cagcatcatc
1501acctgctgca ctgcctggaa aaaaccacta accacgagtt tattgatgag cagatgtttg
1561agcagaactg catggagagt tcaatgcaga actacccatc cacaagaagt ccctcactgt
1621ccagccaccc aggcctcact accacctgct gctcccgtcg tagtaagaag accacacacc
1681tgcccaattc taacctgcca gctactcgcc TGCGCAGCAT GCAAGAGCTC AGCACGATCC
1741ACATCCAGGG CAGTGAGCAG CCCTCCCTCA CAACCAGTCG CTCCAGCCTT AATTTGAAAG
1801CAGACGACGG ACTGAGACCA AACTGCAAAA CATCCCAGAT CACCACAGCC ATCATCAGCA
1861TCCCCACTCC CCCAGCGCTA ACCCCAGAGG GGGAAAGTCG GCCACCCCCT GCCAGCCCAG
1921GCCCCAACAC GAACATTCCT TCCATAGCCA GCAATGTTGT CAAGGTCTCC GCCTTGTTGC
1981CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
2041TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
2101ATATATCTTG TGGAAAGGAC GATAACCCGT TACCCGTCAG ACCCATACGC GTTAAGTCga
2161caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)