Construct: ORF TRCN0000472238
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011664.1_s317c1
- Derived from:
- ccsbBroadEn_00148
- DNA Barcode:
- AGTATTTGCGGTAGTCCAGACGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BAGE (574)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472238
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 574 | BAGE | B melanoma antigen | NM_001187.1 | 100% | 100% | |
| 2 | human | 85316 | BAGE5 | BAGE family member 5 | NM_182484.2 | 97.6% | 97.6% | 6A>G;10G>A;54A>G |
| 3 | human | 85317 | BAGE4 | BAGE family member 4 | NM_181704.1 | 88.3% | 86% | (many diffs) |
| 4 | human | 85318 | BAGE3 | BAGE family member 3 | NM_182481.1 | 36.3% | 34.8% | (many diffs) |
| 5 | human | 85319 | BAGE2 | BAGE family member 2 | NM_182482.2 | 36.3% | 34.8% | (many diffs) |
| 6 | human | 643648 | LINC01189 | long intergenic non-protein... | NR_046203.2 | 9.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 317
- ORF end:
- 446
- ORF length:
- 129
- Sequence:
-
1 GTCGCTTCAT GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG 61 AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC 121 CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG 181 GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 241 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA CAAGTTTGTA 301 CAAAAAAGTT GGCACCATGG CGGCCAGAGC GGTTTTTCTG GCATTGTCTG CCCAGCTGCT 361 CCAAGCCAGG CTGATGAAGG AGGAGTCCCC TGTGGTGAGC TGGAGGTTGG AGCCTGAAGA 421 CGGCACAGCT CTGTGCTTCA TCTTCTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG 481 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 541 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGTATTTGC 601 GGTAGTCCAG ACGACACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 661 agatt