Construct: ORF TRCN0000472239
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013943.1_s317c1
- Derived from:
- ccsbBroadEn_07224
- DNA Barcode:
- ATGAGCATATTTAAATGCTTTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2BC21 (8349)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472239
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8349 | H2BC21 | H2B clustered histone 21 | NM_003528.2 | 99.2% | 100% | 12G>T;15A>T;18A>G |
| 2 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001024599.4 | 94.1% | 97.6% | (many diffs) |
| 3 | human | 8348 | H2BC17 | H2B clustered histone 17 | NM_003527.4 | 92.5% | 99.2% | (many diffs) |
| 4 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_001312653.1 | 91.7% | 98.4% | (many diffs) |
| 5 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_080593.2 | 91.7% | 98.4% | (many diffs) |
| 6 | human | 8343 | H2BC7 | H2B clustered histone 7 | NM_003522.3 | 91.5% | 99.2% | (many diffs) |
| 7 | human | 8341 | H2BC15 | H2B clustered histone 15 | NM_003520.3 | 91.2% | 98.4% | (many diffs) |
| 8 | human | 8344 | H2BC6 | H2B clustered histone 6 | NM_003523.2 | 90.7% | 99.2% | (many diffs) |
| 9 | human | 8340 | H2BC13 | H2B clustered histone 13 | NM_003519.3 | 90.7% | 97.6% | (many diffs) |
| 10 | human | 54145 | H2BS1 | H2B.S histone 1 | NM_017445.3 | 90.7% | 96.8% | (many diffs) |
| 11 | human | 102724334 | LOC102724334 | histone H2B type F-S-like | XM_006723918.4 | 90.7% | 96.8% | (many diffs) |
| 12 | human | 8346 | H2BC10 | H2B clustered histone 10 | NM_003525.2 | 90.4% | 99.2% | (many diffs) |
| 13 | human | 8347 | H2BC4 | H2B clustered histone 4 | NM_003526.2 | 90.4% | 99.2% | (many diffs) |
| 14 | human | 128312 | H2BU1 | H2B.U histone 1 | NM_175055.2 | 90.4% | 96% | (many diffs) |
| 15 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_021063.3 | 89.9% | 98.4% | (many diffs) |
| 16 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_138720.2 | 89.9% | 98.4% | (many diffs) |
| 17 | human | 8970 | H2BC11 | H2B clustered histone 11 | NM_021058.3 | 89.6% | 99.2% | (many diffs) |
| 18 | human | 8345 | H2BC9 | H2B clustered histone 9 | NM_003524.2 | 89.1% | 98.4% | (many diffs) |
| 19 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001161334.1 | 88.5% | 91.7% | (many diffs) |
| 20 | human | 3018 | H2BC3 | H2B clustered histone 3 | NM_021062.2 | 88% | 98.4% | (many diffs) |
| 21 | human | 8342 | H2BC14 | H2B clustered histone 14 | NM_003521.2 | 88% | 96% | (many diffs) |
| 22 | human | 8339 | H2BC8 | H2B clustered histone 8 | NM_003518.3 | 87.3% | 99.2% | (many diffs) |
| 23 | human | 3017 | H2BC5 | H2B clustered histone 5 | XM_005249039.4 | 75.5% | 82.6% | (many diffs) |
| 24 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160944.2 | 70.5% | (many diffs) | |
| 25 | human | 337873 | H2BC20P | H2B clustered histone 20, p... | NR_036461.1 | 62% | (many diffs) | |
| 26 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160915.1 | 22% | (many diffs) | |
| 27 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_027337.2 | 19% | (many diffs) | |
| 28 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160941.1 | 17.6% | (many diffs) | |
| 29 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160942.1 | 16.4% | (many diffs) | |
| 30 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160940.1 | 15.7% | (many diffs) | |
| 31 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160913.1 | 14.3% | (many diffs) | |
| 32 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160914.1 | 11.8% | (many diffs) | |
| 33 | mouse | 319187 | Hist1h2bn | histone cluster 1, H2bn | NM_178201.2 | 93.1% | 98.4% | (many diffs) |
| 34 | mouse | 319180 | Hist1h2bf | histone cluster 1, H2bf | NM_178195.2 | 92.8% | 98.4% | (many diffs) |
| 35 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001177653.1 | 92.5% | 99.2% | (many diffs) |
| 36 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_001290380.1 | 92.5% | 99.2% | (many diffs) |
| 37 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001290530.1 | 92.5% | 99.2% | (many diffs) |
| 38 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_023422.3 | 92.5% | 99.2% | (many diffs) |
| 39 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_178194.4 | 92.5% | 99.2% | (many diffs) |
| 40 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001313878.1 | 92.5% | 98.4% | (many diffs) |
| 41 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001313880.1 | 92.5% | 98.4% | (many diffs) |
| 42 | mouse | 319185 | Hist1h2bl | histone cluster 1, H2bl | NM_178199.2 | 92.5% | 98.4% | (many diffs) |
| 43 | mouse | 319181 | Hist1h2bg | histone cluster 1, H2bg | NM_178196.4 | 92.3% | 99.2% | (many diffs) |
| 44 | mouse | 319182 | Hist1h2bh | histone cluster 1, H2bh | NM_178197.2 | 92% | 98.4% | (many diffs) |
| 45 | mouse | 319183 | Hist1h2bj | histone cluster 1, H2bj | NM_178198.2 | 92% | 98.4% | (many diffs) |
| 46 | mouse | 319186 | Hist1h2bm | histone cluster 1, H2bm | NM_178200.2 | 92% | 98.4% | (many diffs) |
| 47 | mouse | 319184 | Hist1h2bk | histone cluster 1, H2bk | NM_175665.2 | 92% | 97.6% | (many diffs) |
| 48 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_001290466.1 | 91.7% | 96.8% | (many diffs) |
| 49 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | XM_006516690.2 | 91.7% | 96.8% | (many diffs) |
| 50 | mouse | 319178 | Hist1h2bb | histone cluster 1, H2bb | NM_175664.3 | 90.2% | 96% | (many diffs) |
| 51 | mouse | 78303 | Hist3h2ba | histone cluster 3, H2ba | NM_030082.4 | 88.4% | 94.4% | (many diffs) |
| 52 | mouse | 319190 | Hist2h2be | histone cluster 2, H2be | NM_178214.4 | 87.8% | 96.8% | (many diffs) |
| 53 | mouse | 319189 | Hist2h2bb | histone cluster 2, H2bb | NM_175666.2 | 87.5% | 97.6% | (many diffs) |
| 54 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001097979.2 | 86.8% | 91.7% | (many diffs) |
| 55 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001110555.1 | 86.8% | 91.7% | (many diffs) |
| 56 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_178202.2 | 83.5% | 88.4% | (many diffs) |
| 57 | mouse | 319177 | Hist1h2ba | histone cluster 1, H2ba | NM_175663.2 | 81.1% | 88.1% | (many diffs) |
| 58 | mouse | 382522 | Hist3h2bb-ps | histone cluster 3, H2bb, ps... | NR_134309.1 | 71.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 447
- ORF length:
- 378
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgaTC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GCCTGAACCT GCTAAGTCCG CTCCGGCCCC TAAAAAGGGC TCCAAGAAAG 121 CCGTCACCAA AGCCCAGAAG AAAGACGGCA AGAAGCGCAA GCGCAGCCGC AAAGAGAGCT 181 ACTCCATCTA CGTGTACAAG GTGCTGAAGC AGGTCCACCC CGACACCGGC ATCTCGTCCA 241 AGGCCATGGG CATCATGAAC TCCTTCGTCA ACGACATCTT CGAGCGCATC GCGGGAGAGG 301 CTTCCCGCCT GGCGCACTAC AACAAGCGCT CCACCATCAC ATCCCGCGAG ATCCAGACGG 361 CCGTGCGCCT GCTGCTGCCC GGCGAGCTGG CCAAGCACGC CGTGTCCGAG GGCACCAAGG 421 CGGTCACCAA GTACACCAGC TCCAAGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 481 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 541 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATGAGCAT 601 ATTTAAATGC TTTCTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 661 aagatt