Construct: ORF TRCN0000472266
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013912.1_s317c1
- Derived from:
- ccsbBroadEn_11722
- DNA Barcode:
- TAGGATTCGATGTGTTACGACTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- JADE2 (23338)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472266
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23338 | JADE2 | jade family PHD finger 2 | NM_001289984.2 | 100% | 100% | |
| 2 | human | 23338 | JADE2 | jade family PHD finger 2 | NM_015288.6 | 99.8% | 99.8% | 1550_1551insAGC |
| 3 | human | 23338 | JADE2 | jade family PHD finger 2 | XM_017009282.1 | 94.9% | 94.8% | 1550_1567del;1573_1680del |
| 4 | human | 23338 | JADE2 | jade family PHD finger 2 | NM_001308143.2 | 94.8% | 94.8% | 1552_1680del |
| 5 | human | 23338 | JADE2 | jade family PHD finger 2 | XM_005271945.2 | 94.8% | 94.8% | 1552_1680del |
| 6 | human | 23338 | JADE2 | jade family PHD finger 2 | XM_005271946.2 | 94.8% | 94.8% | 1552_1680del |
| 7 | human | 23338 | JADE2 | jade family PHD finger 2 | XM_005271948.4 | 94.8% | 94.8% | 1552_1680del |
| 8 | human | 23338 | JADE2 | jade family PHD finger 2 | XM_011543291.3 | 94.8% | 94.8% | 1552_1680del |
| 9 | human | 23338 | JADE2 | jade family PHD finger 2 | XM_024446007.1 | 93.2% | 91.6% | (many diffs) |
| 10 | human | 23338 | JADE2 | jade family PHD finger 2 | NM_001289985.2 | 93% | 93% | 1_48del;1600_1728del |
| 11 | mouse | 76901 | Jade2 | jade family PHD finger 2 | NM_199299.3 | 83% | 86.5% | (many diffs) |
| 12 | mouse | 76901 | Jade2 | jade family PHD finger 2 | XM_006534469.3 | 83% | 86.5% | (many diffs) |
| 13 | mouse | 76901 | Jade2 | jade family PHD finger 2 | XM_006534470.3 | 83% | 86.5% | (many diffs) |
| 14 | mouse | 76901 | Jade2 | jade family PHD finger 2 | XM_011249309.2 | 83% | 86.5% | (many diffs) |
| 15 | mouse | 76901 | Jade2 | jade family PHD finger 2 | XM_017314832.1 | 83% | 86.5% | (many diffs) |
| 16 | mouse | 76901 | Jade2 | jade family PHD finger 2 | XM_006534468.3 | 79.6% | 82.9% | (many diffs) |
| 17 | mouse | 76901 | Jade2 | jade family PHD finger 2 | XM_006534466.3 | 76.4% | 79.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2439
- ORF length:
- 2373
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agagaagagg cgaaaatact ccatcagcag tgacaactct gacaccactg 121 acagtcatgc gacatctaca tccgcatcaa gatgctccaa actgcccagc agcaccaagt 181 cgggctggcc ccgacagaac gaaaagaagc cctccgaggt tttccggaca gacttgatca 241 cagccatgaa gatcccggac tcataccagc tcagcccgga tgactactac atcctggcag 301 acccatggcg acaggaatgg gagaaaggtg tgcaggtgcc tgccggggca gaggccatcc 361 cagagcccgt ggtgaggatc ctcccaccac tggaaggccc ccctgcccag gcatccccga 421 gcagcaccat gcttggtgag ggctcccagc ctgattggcc agggggcagc cgctatgact 481 tggacgagat tgatgcctac tggctggagc tcatcaactc ggagcttaag gagatggaga 541 ggccggagct ggacgagctg acattagagc gtgtgctgga ggagctggag accctgtgcc 601 accagaatat ggccagggcc attgagacgc aggaggggct gggcatcgag tacgacgagg 661 atgttgtctg cgacgtgtgt cgctctcctg agggcgagga tggcaacgag atggtcttct 721 gtgacaagtg caacgtctgt gtgcatcagg catgctacgg gatcctcaag gtgcccacgg 781 gcagctggct gtgccggacg tgtgccctgg gtgtccagcc aaagtgcctg ctctgcccca 841 agcgaggagg agccttgaag cccactagaa gtgggaccaa gtgggtgcat gtcagctgtg 901 ccctatggat tcctgaggtc agcatcggct gcccagagaa gatggagccc atcaccaaga 961 tctcgcatat cccagccagc cgctgggctc tgtcctgcag cctctgcaag gaatgcacag 1021 gcacctgcat ccagtgttcc atgccttcct gcgtcacagc gttccatgtc acatgcgcct 1081 ttgaccacgg cctggaaatg cggactatat tagcagacaa cgatgaggtc aagttcaagt 1141 cattctgcca ggagcacagt gacgggggcc cacgtaatga gcccacatct gagcccacgg 1201 aacccagcca ggctggcgag gacctggaaa aggtgaccct gcgcaagcag cggctgcagc 1261 agctagagga ggacttctac gagctggtgg agccggctga ggtggctgag cggctggacc 1321 tggctgaggc actggtcgac ttcatctacc agtactggaa gctgaagagg aaagccaatg 1381 ccaaccagcc gctgctgacc cccaagaccg acgaggtgga caacctggcc cagcaggagc 1441 aggacgtcct ctaccgccgc ctgaagctct tcacccatct gcggcaggac ctagagaggg 1501 ttagaaatct gtgctacatg gtgacaaggc gcgagagaac gaaacacgcc atctgcaaac 1561 tccaggagca gatattccac ctgcagatga aacttattga acaggatctg tgtcgagcag 1621 gcctgtccac ctcattcccc atcgatggca ccttcttcaa cagctggctg gcacagtcgg 1681 tgcagatcac agcagagaac atggccatga gcgagtggcc actgaacaat gggcaccgcg 1741 aggaccctgc tccagggctg ctgtcagagg aactgctgca ggacgaggag acactgctca 1801 gcttcatgcg ggacccctcg ctgcgacctg gtgaccctgc taggaaggcc cgaggccgca 1861 cccgcctgcc tgccaagaag aaaccaccac caccaccacc gcaggacggg cctggttcac 1921 ggacgactcc agacaaagcc cccaagaaga cctggggcca ggatgcaggc agtggcaagg 1981 ggggtcaagg gccacctacc aggaagccac cacgtcggac atcttcTCAC TTGCCGTCCA 2041 GCCCTGCAGC CGGGGACTGT CCCATCCTAG CCACCCCTGA AAGCCCCCCG CCACTGGCCC 2101 CTGAGACCCC GGACGAGGCA GCCTCAGTAG CTGCTGACTC AGATGTCCAA GTGCCTGGCC 2161 CTGCAGCAAG CCCTAAGCCT TTGGGCCGGC TCCGGCCACC CCGCGAGAGC AAGGTAACCC 2221 GGAGATTGCC GGGTGCCAGG CCTGATGCTG GGATGGGACC ACCTTCAGCT GTGGCTGAGA 2281 GGCCCAAGGT CAGCCTGCAT TTTGACACTG AGACTGATGG CTACTTCTCT GATGGGGAGA 2341 TGAGCGACTC AGATGTAGAG GCCGAGGACG GTGGGGTGCA GCGGGGTCCC CGGGAGGCAG 2401 GGGCAGAGGA GGTGGTCCGC ATGGGCGTAC TGGCCTCCTA CCCAACTTTC TTGTACAAAG 2461 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2521 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2581 ACGATAGGAT TCGATGTGTT ACGACTGGAC GCGTTAAGTC gacaatcaac ctctggatta 2641 caaaatttgt gaaagatt