Construct: ORF TRCN0000472271
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017024.1_s317c1
- Derived from:
- ccsbBroadEn_13334
- DNA Barcode:
- GCTTAGCCCCTCTCTGAAATGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DENND2C (163259)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472271
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 163259 | DENND2C | DENN domain containing 2C | NM_001256404.2 | 87.8% | 87.8% | 6T>A;1341C>T;1924_2259del |
| 2 | human | 163259 | DENND2C | DENN domain containing 2C | NM_198459.4 | 81.7% | 81.6% | (many diffs) |
| 3 | mouse | 329727 | Dennd2c | DENN/MADD domain containing 2C | XM_006501606.3 | 34.6% | 35.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2514
- ORF length:
- 2448
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agttggtttt tctcgtacta ctgttcagac actgtcaaga agccactgca 121 aaaacatcaa acaaaaaatt tctcaatggg aaggaagggc taatggtata tctaatccag 181 aaaagtggtg tccaaaggac tttggagtga gatataactg tcaccaagag atccgtctta 241 agaaaaatcc tatagctgag agaaagagca aaaacttgga tgtaaccagc cgtgaaaatg 301 tgggtctaga tataaatgaa aataccaaaa gccatgatca aagtgagaat gaaaataaga 361 aacatgaata tgacgataca cacttcttta aaaatgaatc agaatccaac tgggtatgtt 421 ctcgggtcaa agaaattgaa agctgtaaag aagatgtctt agatccagag acttcattac 481 ctccaggaaa cttctatacc tcacaaatac tgtggaagaa aatagaagca cttcccccag 541 ataaactctt aaatttggct ttagaacatt gtgactcttc agaaaaagaa ctgaacttca 601 gagttctgga tagttcatac ggaataacca agagcttaga aaatatttac tctgaacctg 661 aggggcaaga atgtggacct tccataaatc ctttgccaaa acctcgtagg acattcagat 721 atttatccga atctggtgtt acgccgtata aagaaagaaa ctgtgacaaa aaatactgtg 781 aaaataactc ttgtgcacaa tcttctttgg cctcttctca ggaacctgaa ccaaagaaat 841 atggtggaaa aatcagagga agatctaaaa ggaaatcctt tgaatttgag gatattcagc 901 actttcgaaa tcggaactca cagacgattc gtgaagaact tggaagaaat tctgggtcag 961 cactttatta cacacagtct gaggacaata tctatgaaga tatcatatat cccaccaaag 1021 aaaatccata tgaagatatt ccagtgcagc ctttacctat gtggagatcc ccttcagcat 1081 ggaagctacc acccgctaaa agtgctttta aagcacccaa gctccctcca aaacctcagt 1141 tccttcaccg gaagactatg gaagtaaaga actcacaggc ttatttgcgg tcaaagctta 1201 caaaagatac aactttgccg gtcactttaa cggaatggaa gcttttccga gctggtgaag 1261 ttgcaaacac gaaaaggaaa aatcttccaa ggcttgtatt gaaaatagat gacatatttg 1321 aatctaaaag agggaagaag aaggtaaagt tacattctta cactggaaag gaattacctc 1381 cgacaaaagg tgaaaccagt gggaatgaaa gtgatgccga gtatctgcca aagaatcgcc 1441 ataaacgctt agcacaactg caaccgtctt ccaagaggaa tcctcactac cagaccttgg 1501 agcgggatct tattgaatta caggagcagc agctgtttga actttttgtg gtggtgtctc 1561 tacagaagaa accatcagga ataagctata ttccccaggt catacaacaa ttccctggca 1621 aggatgatca tggctataag cagtccaaag acatggaaga gagacttaaa gttattccaa 1681 aattttgttt tcctgattca aaggactgga tgccaacctc agaactcaag agtgaaacat 1741 tctcctttgt cttgactggt gaagatggaa gccggtggtt tggttactgt aagaagctct 1801 tgccagtagg caaaggaaag cgactccctg aggtatactg catggttagt cgcctaggct 1861 gcttcaatct tttttcaaag attctggatg aagtagagaa gagaagagaa atgtctccag 1921 cccttgttta cccattcatg cgaagtgtca tggaagctcc tttcccagct cctggacgca 1981 ccatcacact ccaggaccta cccattgaag aggtgctgat agttgatctc tgtgcagaca 2041 agttcttaca ggaggtatct gatgaggatg aaattctacc accaaaactt caagctgccc 2101 tgatgcagat tttggaagaa cgaaatgaaa TCTTGACTCA GGAGCAGAAT TTTTCACAAG 2161 ATGTGACACT CAACTCTCTG GTGTCCGAAG CATTTGTCAG GTTTTTTGTG GAGTTGGTAG 2221 GACATTATTC TTTGAACATG ACTGTCACTG AGCGTGGGGA GCGTGTTTTC CAAAGGGAAC 2281 CATTCCGTAA GTCCCACACC TCCCGAAGTG TACGCCACTT CCTGGATCTC TTCATGGAAA 2341 CTCAGATGTT TGCAGGATTC ATCCAGGACC GAGAGCTTCG GAAAAGTGGA GTTAAAGGTT 2401 TGTTTGAGAT CCGGGCCATC CAGTATTTGG AAACAATTCC TGAGTCGGAG CCCAGTGGGA 2461 TGAATAGAAT TTTGCGGAGT CTTGGAAGCA AAATGAAATT TCTGCAAAAG AAATGCCCAA 2521 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2581 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2641 TATCTTGTGG AAAGGACGAG CTTAGCCCCT CTCTGAAATG CACACGCGTT AAGTCgacaa 2701 tcaacctctg gattacaaaa tttgtgaaag att