Construct: ORF TRCN0000472281

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016193.1_s317c1
Derived from:
ccsbBroadEn_12667
DNA Barcode:
GACTAAAAACAGGACTTTACAAGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LRRTM4 (80059)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472281
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80059 LRRTM4 leucine rich repeat transme... NM_024993.6 39.9% 39.9% 1_933del
2 human 80059 LRRTM4 leucine rich repeat transme... NM_001282928.3 39.8% 39.8% 1_936del
3 human 80059 LRRTM4 leucine rich repeat transme... NM_001134745.2 34.9% 34.9% 1_933del;1552A>G;1554_1770delinsA
4 human 80059 LRRTM4 leucine rich repeat transme... NM_001282924.2 34.9% 34.9% 1_933del;1552A>G;1554_1770delinsA
5 human 80059 LRRTM4 leucine rich repeat transme... NM_001330370.1 34.9% 34.8% 1_936del;1555A>G;1557_1773delinsA
6 mouse 243499 Lrrtm4 leucine rich repeat transme... NM_178731.5 34.3% 37% (many diffs)
7 mouse 243499 Lrrtm4 leucine rich repeat transme... NM_001282102.1 34.3% 36.9% (many diffs)
8 mouse 243499 Lrrtm4 leucine rich repeat transme... XM_006506089.3 34.3% 36.9% (many diffs)
9 mouse 243499 Lrrtm4 leucine rich repeat transme... XM_011241336.2 34.1% 36.8% (many diffs)
10 mouse 243499 Lrrtm4 leucine rich repeat transme... XM_006506088.2 32.8% 35.3% (many diffs)
11 mouse 243499 Lrrtm4 leucine rich repeat transme... NM_001347261.1 30.1% 32.3% (many diffs)
12 mouse 243499 Lrrtm4 leucine rich repeat transme... XM_006506085.1 30.1% 32.3% (many diffs)
13 mouse 243499 Lrrtm4 leucine rich repeat transme... XM_006506086.2 30.1% 32.3% (many diffs)
14 mouse 243499 Lrrtm4 leucine rich repeat transme... XM_006506087.2 30.1% 32.3% (many diffs)
15 mouse 243499 Lrrtm4 leucine rich repeat transme... NM_001134743.1 30% 32.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
687
ORF length:
621
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtg ggaatgcagt cggagcattt gtcctttatt ttattggctt aagaatttca
121aaggaaataa ggaaagcacc atgatatgtg cgggacctaa gcacatccag ggtgaaaagg
181ttagtgatgc agtggaaaca tataatatct gttctgaagt ccaggtggtc aacacagaaa
241gatcacacct ggtgccccaa actccccaga aacctctgat tatccctaga cctaccatct
301tcaaacctga cgtcacccaa tccacctttg aaacaccaag cccTTCCCCA GGGTTTCAGA
361TTCCTGGCGC AGAGCAAGAG TATGAGCATG TTTCATTTCA CAAAATTATT GCCGGGAGTG
421TGGCTCTCTT TCTCTCAGTG GCCATGATCC TCTTGGTGAT CTATGTGTCT TGGAAACGCT
481ACCCAGCCAG CATGAAACAA CTCCAGCAAC ACTCTCTTAT GAAGAGGCGG CGGAAAAAGG
541CCAGAGAGTC TGAAAGACAA ATGAATTCCC CTTTACAGGA GTATTATGTG GACTACAAGC
601CTACAAACTC TGAGACCATG GATATATCGG TTAATGGATC TGGGCCCTGC ACATATACCA
661TCTCTGGCTC CAGGGAATGT GAGGTATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG
721GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
781AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGACTAAAA
841ACAGGACTTT ACAAGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
901aagatt

Download FASTA (ORF) (Full)