Construct: ORF TRCN0000472284
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016008.1_s317c1
- Derived from:
- ccsbBroadEn_04554
- DNA Barcode:
- AACATCATACGTTCCGAATAGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYT12 (91683)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472284
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91683 | SYT12 | synaptotagmin 12 | NM_001177880.2 | 100% | 100% | |
| 2 | human | 91683 | SYT12 | synaptotagmin 12 | NM_177963.4 | 100% | 100% | |
| 3 | human | 91683 | SYT12 | synaptotagmin 12 | XM_011545347.1 | 100% | 100% | |
| 4 | human | 91683 | SYT12 | synaptotagmin 12 | XM_006718737.4 | 95.8% | 95.8% | 1_54del |
| 5 | human | 91683 | SYT12 | synaptotagmin 12 | XM_011545346.3 | 92.3% | 92.3% | 1_105del |
| 6 | human | 91683 | SYT12 | synaptotagmin 12 | XM_024448766.1 | 86.4% | 86.4% | 1_198del |
| 7 | human | 91683 | SYT12 | synaptotagmin 12 | NM_001318773.2 | 72.6% | 72.6% | 0_1ins345 |
| 8 | human | 91683 | SYT12 | synaptotagmin 12 | NM_001318775.2 | 72.6% | 72.6% | 0_1ins345 |
| 9 | human | 91683 | SYT12 | synaptotagmin 12 | XM_017018547.1 | 72.6% | 72.6% | 0_1ins345 |
| 10 | human | 91683 | SYT12 | synaptotagmin 12 | XM_017018548.1 | 72.6% | 72.6% | 0_1ins345 |
| 11 | mouse | 171180 | Syt12 | synaptotagmin XII | NM_134164.5 | 87.7% | 95% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1329
- ORF length:
- 1263
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgtggatgtg gcagaatacc atctgagcgt catcaagagc ccccctggct 121 gggaggtggg tgtctatgct gcaggggccc tggccctgct gggaatcgca gctgtgagcc 181 tgtggaagct ctggacgtcg gggagcttcc ccagcccctc tccgttcccc aattacgact 241 acaggtacct tcagcagaag tacggcgaga gctgcgcaga ggccagggag aagagagtgc 301 ctgcctggaa tgcccagcgg gccagcacgc ggggaccacc cagccgcaaa ggcagtctca 361 gcattgagga cacctttgag agcatcagtg aactggggcc tctggagctg atgggccggg 421 agttggacct ggccccctat gggaccctcc ggaagtccca gtcggccgac tccctgaact 481 ccatctcctc cgtgagcaac acctttgggc aggacttcac actgggccag gtggaggtga 541 gcatggagta cgacactgcc tcccacacgc tgaacgtggc ggtgatgcag ggcaaggacc 601 tcctggagcg ggaggaggcc agcttcgagt cctgcttcat gcgcgtcagc ctgctgccgg 661 acgagcagat cgtgggcatt tctcggatcc agagaaatgc ctactccatc ttctttgatg 721 agaagttctc catccccctg gatcccacag ccctggagga gaagagcctg cggttttctg 781 tatttggcat cgatgaggat gagcgcaacg tcagcacggg ggtggtggag ctgaagcttt 841 ctgtgcttga cctcccgctg cagcccttca gtggctggct ctatttacag gaccagaaca 901 aggccgccga tgctgtgggg gagatcctgc tctccctcag ctacctcccc acagccgagc 961 gcctcaccgt ggtcgtggtt aaggccaaga acctcatctg gaccaacgac aagaccacag 1021 cggacCCCTT CGTCAAGGTG TACCTGCTGC AGGATGGGAG GAAGATGAGC AAAAAGAAGA 1081 CAGCCGTGAA GAGGGATGAC CCCAACCCGG TGTTCAACGA AGCCATGATC TTCTCGGTGC 1141 CAGCCATTGT GCTCCAGGAC CTGTCTCTCC GCGTGACGGT GGCTGAGAGC AGCAGCGACG 1201 GCCGTGGGGA CAACGTGGGC CATGTCATCA TTGGGCCGTC AGCCAGTGGC ATGGGAACCA 1261 CACATTGGAA CCAGATGTTG GCCACGCTGC GCAGGCCCGT GTCCATGTGG CACGCTGTCC 1321 GGCGAAACTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1381 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1441 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAACATC ATACGTTCCG AATAGGCGAC 1501 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt