Construct: ORF TRCN0000472312
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017990.1_s317c1
- Derived from:
- ccsbBroadEn_03130
- DNA Barcode:
- TTCTAGCCAACCGTTCTGCATAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NXPH1 (30010)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472312
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30010 | NXPH1 | neurexophilin 1 | NM_152745.3 | 100% | 100% | |
| 2 | mouse | 18231 | Nxph1 | neurexophilin 1 | NM_008751.5 | 92.7% | 99.6% | (many diffs) |
| 3 | mouse | 18231 | Nxph1 | neurexophilin 1 | XM_006505010.3 | 92.7% | 99.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 879
- ORF length:
- 813
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggctgcgtgc tggtacgtgc ttttcctcct gcagcccacc gtctacttgg 121 tcacatgtgc caatttaacg aacggtggaa agtcagaact tctgaaatca ggaagcagca 181 aatccacact aaagcacata tggacagaaa gcagcaaaga cttgtctatc agccgactcc 241 tgtcacagac ttttcgtggc aaagagaatg atacagattt ggacctgaga tatgacaccc 301 cagaacctta ttctgagcaa gacctctggg actggctgag gaactccaca gaccttcaag 361 agcctcggcc cagggccaag agaaggccca ttgttaaaac gggcaagttt aagaaaatgt 421 ttggatgggg cgattttcat tccaacatca aaacagtgaa gctgaacctg ttgataactg 481 ggaaAATTGT AGATCATGGC AATGGGACAT TTAGTGTTTA TTTCAGGCAT AATTCAACTG 541 GTCAAGGGAA TGTATCTGTC AGCTTGGTAC CCCCTACAAA AATCGTGGAA TTTGACTTGG 601 CACAACAAAC CGTGATTGAT GCCAAAGATT CCAAGTCTTT TAATTGTCGC ATTGAATATG 661 AAAAGGTTGA CAAGGCTACC AAGAACACAC TCTGCAACTA TGACCCTTCA AAAACCTGTT 721 ACCAGGAGCA AACCCAAAGT CATGTATCCT GGCTCTGCTC CAAGCCCTTT AAGGTGATCT 781 GTATTTACAT TTCCTTTTAT AGTACAGATT ATAAACTGGT ACAGAAAGTG TGCCCTGACT 841 ACAACTACCA CAGTGACACA CCTTACTTTC CCTCGGGATG CCCAACTTTC TTGTACAAAG 901 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 961 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1021 ACGATTCTAG CCAACCGTTC TGCATAGTAC GCGTTAAGTC gacaatcaac ctctggatta 1081 caaaatttgt gaaagatt