Construct: ORF TRCN0000472383
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009930.1_s317c1
- Derived from:
- ccsbBroadEn_05292
- DNA Barcode:
- AGTTTACATGTATCCGCCAGTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MSRB3 (253827)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472383
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 253827 | MSRB3 | methionine sulfoxide reduct... | NM_001031679.3 | 100% | 100% | |
| 2 | human | 253827 | MSRB3 | methionine sulfoxide reduct... | NM_001193460.2 | 100% | 100% | |
| 3 | human | 253827 | MSRB3 | methionine sulfoxide reduct... | NM_001193461.1 | 100% | 100% | |
| 4 | human | 253827 | MSRB3 | methionine sulfoxide reduct... | XM_024448918.1 | 100% | 100% | |
| 5 | human | 253827 | MSRB3 | methionine sulfoxide reduct... | XM_024448919.1 | 100% | 100% | |
| 6 | human | 253827 | MSRB3 | methionine sulfoxide reduct... | XM_024448920.1 | 100% | 100% | |
| 7 | human | 253827 | MSRB3 | methionine sulfoxide reduct... | XM_024448921.1 | 100% | 100% | |
| 8 | human | 253827 | MSRB3 | methionine sulfoxide reduct... | NM_198080.4 | 86.8% | 83.4% | (many diffs) |
| 9 | human | 253827 | MSRB3 | methionine sulfoxide reduct... | XM_024448922.1 | 31.3% | 29.2% | (many diffs) |
| 10 | mouse | 320183 | Msrb3 | methionine sulfoxide reduct... | NM_177092.4 | 85.8% | 92% | (many diffs) |
| 11 | mouse | 320183 | Msrb3 | methionine sulfoxide reduct... | XM_006513764.3 | 63.4% | 67.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 621
- ORF length:
- 555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgcattcaac ctgctgcatt tggtgacaaa gagccagcca gtagcccttc 121 gagcctgtgg gcttcccTCA GGGTCGTGTA GGGATAAAAA GAACTGTAAG GTGGTCTTTT 181 CCCAGCAGGA ACTGAGGAAG CGGCTAACAC CCCTGCAGTA CCATGTCACT CAGGAGAAAG 241 GGACCGAAAG TGCCTTTGAA GGAGAATACA CACATCACAA AGATCCTGGA ATATATAAAT 301 GTGTTGTTTG TGGAACTCCA TTGTTTAAGT CAGAAACCAA ATTTGACTCC GGTTCAGGTT 361 GGCCTTCATT CCACGATGTG ATCAATTCTG AGGCAATCAC ATTCACAGAT GACTTTTCCT 421 ATGGGATGCA CAGGGTGGAA ACAAGCTGCT CTCAGTGTGG TGCTCACCTT GGGCACATTT 481 TTGATGATGG GCCTCGTCCA ACTGGGAAAA GATACTGCAT AAATTCGGCT GCCTTGTCTT 541 TTACACCTGC GGATAGCAGT GGCACCGCCG AGGGAGGCAG TGGGGTCGCC AGCCCGGCCC 601 AGGCAGACAA AGCGGAGCTC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 661 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 721 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGTT TACATGTATC 781 CGCCAGTTAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt