Construct: ORF TRCN0000472398
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007598.2_s317c1
- Derived from:
- ccsbBroadEn_01291
- DNA Barcode:
- TATATCCATGATGTCTATAAATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP2K6 (5608)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472398
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5608 | MAP2K6 | mitogen-activated protein k... | NM_002758.4 | 100% | 100% | |
| 2 | human | 5608 | MAP2K6 | mitogen-activated protein k... | XM_011525026.2 | 98.2% | 97.6% | (many diffs) |
| 3 | human | 5608 | MAP2K6 | mitogen-activated protein k... | NM_001330450.2 | 83.2% | 83.2% | 0_1ins168 |
| 4 | human | 5608 | MAP2K6 | mitogen-activated protein k... | XM_005257516.2 | 83.2% | 83.2% | 0_1ins168 |
| 5 | human | 5608 | MAP2K6 | mitogen-activated protein k... | XM_006721975.3 | 83.2% | 83.2% | 0_1ins168 |
| 6 | human | 5608 | MAP2K6 | mitogen-activated protein k... | XM_011525027.3 | 83.2% | 83.2% | 0_1ins168 |
| 7 | mouse | 26399 | Map2k6 | mitogen-activated protein k... | NM_011943.2 | 91% | 97.9% | (many diffs) |
| 8 | mouse | 26399 | Map2k6 | mitogen-activated protein k... | XM_011249034.2 | 81.2% | 86% | (many diffs) |
| 9 | mouse | 26399 | Map2k6 | mitogen-activated protein k... | XM_006533377.3 | 71.5% | 77.5% | (many diffs) |
| 10 | mouse | 26399 | Map2k6 | mitogen-activated protein k... | XM_006533378.3 | 71.5% | 77.5% | (many diffs) |
| 11 | mouse | 26399 | Map2k6 | mitogen-activated protein k... | XM_011249035.2 | 71.5% | 77.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1068
- ORF length:
- 1002
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tcagtcgaaa ggcaagaagc gaaaccctgg ccttaaaatt ccaaaagaag 121 catttgaaca acctcagacc agttccacac cacctcgaga tttagactcc aaggcttgca 181 tttctattgg aaatcagaac tttgaggtga aggcagatga cctggagcct ataatggaac 241 tgggacgagg tgcgtacggg gtggtggaga agatgcggca cgtgcccagc gggcagatca 301 tggcagtgaa gcggatccga gccacagtaa atagccagga acagaaacgg ctactgatgg 361 atttggatat ttccatgagg acggtggact gtccattcac tgtcaccttt tatggcgcac 421 tgtttcggga gggtgatgtg tggatctgca tggagctcat ggatacatca ctagataaat 481 tctacaaaca agttattgat aaaggccaga caattccaga ggacatctta gggaaaatag 541 cagtttctat tgtaaaagca ttagaacatt tacatagtaa gctgtctgtc attcacagag 601 acgtcaagcc ttctaatgta ctcatcaatg ctctcggtca agtgaagatg tgcgattttg 661 gaatcagtgg ctacttggtg gactctgttg cTAAAACAAT TGATGCAGGT TGCAAACCAT 721 ACATGGCCCC TGAAAGAATA AACCCAGAGC TCAACCAGAA GGGATACAGT GTGAAGTCTG 781 ACATTTGGAG TCTGGGCATC ACGATGATTG AGTTGGCCAT CCTTCGATTT CCCTATGATT 841 CATGGGGAAC TCCATTTCAG CAGCTCAAAC AGGTGGTAGA GGAGCCATCG CCACAACTCC 901 CAGCAGACAA GTTCTCTGCA GAGTTTGTTG ACTTTACCTC ACAGTGCTTA AAGAAGAATT 961 CCAAAGAACG GCCTACATAC CCAGAGCTAA TGCAACATCC ATTTTTCACC CTACATGAAT 1021 CCAAAGGAAC AGATGTGGCA TCTTTTGTAA AACTGATTCT TGGAGACTAC CCAACTTTCT 1081 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1141 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1201 GTGGAAAGGA CGATATATCC ATGATGTCTA TAAATATACG CGTTAAGTCg acaatcaacc 1261 tctggattac aaaatttgtg aaagatt