Transcript: Mouse NM_011943.2

Mus musculus mitogen-activated protein kinase kinase 6 (Map2k6), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Map2k6 (26399)
Length:
2064
CDS:
289..1293

Additional Resources:

NCBI RefSeq record:
NM_011943.2
NBCI Gene record:
Map2k6 (26399)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_011943.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000025260 CCGCGGACTTTGTTGACTTTA pLKO.1 1139 CDS 100% 13.200 18.480 N Map2k6 n/a
2 TRCN0000278600 CCGCGGACTTTGTTGACTTTA pLKO_005 1139 CDS 100% 13.200 18.480 N Map2k6 n/a
3 TRCN0000025262 GCCACAGTTAATAGCCAGGAA pLKO.1 544 CDS 100% 2.640 2.112 N Map2k6 n/a
4 TRCN0000278599 GCCACAGTTAATAGCCAGGAA pLKO_005 544 CDS 100% 2.640 2.112 N Map2k6 n/a
5 TRCN0000025259 GCCCACATATCCAGAGCTTAT pLKO.1 1194 CDS 100% 10.800 7.560 N Map2k6 n/a
6 TRCN0000278598 GCCCACATATCCAGAGCTTAT pLKO_005 1194 CDS 100% 10.800 7.560 N Map2k6 n/a
7 TRCN0000025261 GCCAAACAATTCCAGAGGATA pLKO.1 728 CDS 100% 4.950 3.465 N Map2k6 n/a
8 TRCN0000278708 GCCAAACAATTCCAGAGGATA pLKO_005 728 CDS 100% 4.950 3.465 N Map2k6 n/a
9 TRCN0000025263 GCCTTCTAATGTGCTCATTAA pLKO.1 831 CDS 100% 1.320 0.924 N Map2k6 n/a
10 TRCN0000297507 GCCTTCTAATGTGCTCATTAA pLKO_005 831 CDS 100% 1.320 0.924 N Map2k6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_011943.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01291 pDONR223 100% 91% 97.9% None (many diffs) n/a
2 ccsbBroad304_01291 pLX_304 0% 91% 97.9% V5 (many diffs) n/a
3 TRCN0000472398 TATATCCATGATGTCTATAAATAT pLX_317 37.8% 91% 97.9% V5 (many diffs) n/a
4 ccsbBroadEn_14810 pDONR223 0% 91% 97.9% None (many diffs) n/a
5 ccsbBroad304_14810 pLX_304 0% 91% 97.9% V5 (many diffs) n/a
6 TRCN0000467778 GAACAGTGCGAGCGACACCTGAGA pLX_317 34.2% 91% 97.9% V5 (many diffs) n/a
7 TRCN0000492175 CACTGTTGATGCCTACAGTTGAGT pLX_317 34.2% 90.7% 97.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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