Construct: ORF TRCN0000472407
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007275.1_s317c1
- Derived from:
- ccsbBroadEn_02655
- DNA Barcode:
- GGCTATGTTATACTTACGATCTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF24 (11237)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472407
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11237 | RNF24 | ring finger protein 24 | NM_001134337.3 | 100% | 100% | |
| 2 | human | 11237 | RNF24 | ring finger protein 24 | NM_001321749.2 | 100% | 100% | |
| 3 | human | 11237 | RNF24 | ring finger protein 24 | NM_007219.5 | 100% | 100% | |
| 4 | human | 11237 | RNF24 | ring finger protein 24 | XM_011529146.2 | 90.7% | 90.7% | 1_45del |
| 5 | human | 11237 | RNF24 | ring finger protein 24 | NM_001134338.3 | 87.5% | 87.5% | 1_63del |
| 6 | human | 11237 | RNF24 | ring finger protein 24 | XM_011529145.3 | 87.5% | 87.5% | 1_63del |
| 7 | human | 11237 | RNF24 | ring finger protein 24 | XM_017027620.1 | 87.5% | 87.5% | 1_63del |
| 8 | human | 11237 | RNF24 | ring finger protein 24 | XM_017027621.1 | 87.5% | 87.5% | 1_63del |
| 9 | human | 11237 | RNF24 | ring finger protein 24 | XM_011529147.2 | 63.9% | 61.5% | (many diffs) |
| 10 | human | 11237 | RNF24 | ring finger protein 24 | XM_017027622.2 | 63.9% | 61.5% | (many diffs) |
| 11 | mouse | 51902 | Rnf24 | ring finger protein 24 | NM_178607.4 | 92.5% | 97.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 510
- ORF length:
- 444
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ctcggatttc ccacattaca acttcaggat gccTAATATT GGATTCCAGA 121 ATCTGCCTCT CAACATATAT ATTGTGGTTT TTGGTACTGC TATATTTGTC TTCATCCTTA 181 GTTTACTCTT CTGTTGCTAC TTGATTAGGC TAAGACATCA AGCACACAAA GAATTTTATG 241 CCTACAAACA GGTTATATTA AAAGAGAAAG TAAAAGAATT GAATTTACAT GAGCTCTGTG 301 CAGTGTGCCT AGAAGACTTC AAGCCTCGAG ATGAGTTGGG GATTTGCCCA TGTAAGCACG 361 CCTTCCACAG AAAGTGCCTT ATTAAGTGGC TGGAGGTTCG TAAAGTGTGT CCCCTGTGCA 421 ACATGCCAGT TCTACAGCTG GCCCAGTTGC ACAGTAAGCA GGACCGTGGA CCCCCTCAGG 481 GGCCCCTTCC TGGGGCAGAG AACATTGTAT ACCCAACTTT CTTGTACAAA GTGGTTGATA 541 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 601 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGCTA 661 TGTTATACTT ACGATCTGTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 721 tgaaagatt