Construct: ORF TRCN0000472417
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008046.1_s317c1
- Derived from:
- ccsbBroadEn_04327
- DNA Barcode:
- CCCCGGTGAGCGGCCGACGTAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANKRD27 (84079)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472417
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84079 | ANKRD27 | ankyrin repeat domain 27 | NM_032139.3 | 100% | 100% | |
| 2 | human | 84079 | ANKRD27 | ankyrin repeat domain 27 | XM_006723412.2 | 100% | 100% | |
| 3 | human | 84079 | ANKRD27 | ankyrin repeat domain 27 | XM_017027349.1 | 81.3% | 81.3% | 0_1ins588 |
| 4 | mouse | 245886 | Ankrd27 | ankyrin repeat domain 27 (V... | NM_178263.3 | 30.7% | 32.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3216
- ORF length:
- 3150
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tctgtatgat gaagacctcc tgaaaaatcc tttctatctg gctctgcaaa 121 agtgccgccc tgacttgtgc agcaaagtgg cccaaatcca tggcattgtc ttagtaccct 181 gcaaaggaag cctgtcgagc agcatccagt ctacttgtca gtttgagtcc tacattttga 241 tacctgtgga agagcatttt cagaccttaa atggaaagga tgtctttatt caagggaaca 301 ggattaaatt aggagctggt tttgcctgtc ttctctcagt gcccattctc tttgaagaaa 361 ctttctacaa tgaaaaagaa gagagtttca gcatcctgtg tatagcccat cctttggaaa 421 agagagagag ttcagaagag cctttggcac cctcagatcc cttttccctg aaaaccattg 481 aagatgtgag agagttcttg ggaagacact ccgagcgatt tgacaggaac atcgcctctt 541 tccatcgaac attccgagaa tgcgagagaa agagcctccg tcaccacata gactcagcga 601 atgctctcta caccaaatgc ctccagcagc ttctgaggga ctctcacctg aaaatgctcg 661 ccaagcagga ggcccagatg aacctgatga agcaggcagt ggagatatac gtccatcatg 721 aaatttacaa cctgatcttt aaatacgtgg ggaccatgga ggcaagtgag gatgcggcct 781 ttaacaaaat cacaagaagc cttcaagatc ttcagcagaa agatattggt gtgaaaccgg 841 agttcagctt taacatacct cgtgccaaaa gagagctggc tcagctgaac aaatgcacct 901 ccccacagca gaagcttgtc tgcttgcgaa aagtggtgca gctcattaca cagtctccaa 961 gccagagagt gaacctggag accatgtgtg ctgatgatct gctatcagtc ctgttatact 1021 tgcttgtgaa aacggagatc cctaattgga tggcaaattt gagttacatc aaaaacttca 1081 ggtttagcag cttggcaaag gatgaactgg gatactgcct gacctcattc gaagctgcca 1141 ttgaatatat tcggcaagga agcctctctg ctaaaccccc tgagtctgag ggatttggag 1201 acaggctgtt ccttaagcag agaatgagct tactctctca gatgacttcg tctcccaccg 1261 actgcctgtt taagcacatt gcatcaggta accagaaaga agtggagaga cttctgagcc 1321 aagaggacca tgataaagat accgtccaaa agatgtgtca ccctctctgc ttctgcgatg 1381 actgtgagaa actcgtctct gggaggttga atgatccctc agttgtcact ccattctcca 1441 gagacgacag ggggcacacc cctctccatg tggctgctgt ctgtgggcag gcatccctca 1501 tcgacctcct ggtttccaag ggcgccatgg taaatgccac agactaccat ggagccactc 1561 cgctccacct ggcctgtcag aagggctacc agagcgtgac gctgctgctg ctgcactaca 1621 aggccagcgc ggaagtgcag gacaacaatg ggaatacgcc actccacctg gcctgcacct 1681 acggccacga ggactgtgtg aaggctctgg tttactacga cgtggagtcg tgcagacttg 1741 acattggcaa tgagaaagga gacacccctc tacacattgc tgcccgctgg ggctaccaag 1801 gcgtcataga gacattgctg cagaacggag cgtccaccga gatccagaac agactgaagg 1861 agacgcccct caagtgtgca ttaaactcaa agattctgtc tgtaatggaa gcctatcacc 1921 tgtccttcga gaggaggcag aagtcgtccg aggcccctgt gcagtccccg cagcgctccg 1981 tggactccat cagccaagag tcctccactt ccagcttctc ctccatgtca gccagctcaa 2041 ggcaggagga gaccaagaag gactacagag aggtagaaaa acttttgaga gcagttgctg 2101 atggagatct agaaatggtg cgttacctgt tggaatggac agaggaggac ctggaggatg 2161 cggaggacac tgtcagtgca gcggaccccg aattctgtca cccgttgtgc cagtgcccca 2221 agtgtgcccc agctcagaag aggctggcga aggttcctgc cagtgggctt ggtgtgaacg 2281 tgaccagcca ggacggctcc tccccgctgc atgtcgccgc cctgcacggc cgggcggacc 2341 tcatccccct cctgctgaag cacggggcca acgcaggtgc caggaacgca gaccaagccg 2401 tcccgctcca cctggcctgc cagcagggcc actttcaggt ggtgaagtgt ctgttagatt 2461 cgaatgcaaa acccaataag aaggacctca gtggaaacac gcccctcatt tacgcctgct 2521 ccggtggcca tcacgagctt gtggcactgc tgctacagca cggggcctcc attaacgctt 2581 ctaacaataa gggcaacaca gcgctgcacg aggctgtgat tgaaaagcac gtcttcgtgg 2641 tagagctgct tctgctccac ggagcgtcag ttcaggtgct gaacaagcgg cagcgcacgg 2701 ctgtagactg tgctgaacag aattcaaaaa taatggaatt gcttcaggtg gtaccaagct 2761 gtgttgcTTC ATTAGATGAT GTGGCTGAAA CTGACCGCAA GGAGTATGTC ACTGTTAAGA 2821 TCAGGAAAAA ATGGAACTCA AAACTGTATG ATCTACCAGA TGAGCCTTTT ACAAGACAGT 2881 TTTACTTTGT CCACTCAGCT GGTCAGTTTA AGGGAAAGAC TTCAAGGGAG ATTATGGCAA 2941 GAGATAGAAG TGTCCCTAAT TTAACCGAAG GTTCTTTGCA TGAGCCAGGG AGGCAAAGTG 3001 TCACACTGAG ACAGAATAAC CTGCCAGCTC AGAGTGGATC TCATGCTGCT GAGAAAGGCA 3061 ACAGCGACTG GCCAGAGAGG CCTGGACTGA CACAGACTGG CCCTGGACAC AGACGGATGC 3121 TGCGGAGACA CACGGTAGAG GATGCGGTCG TGTCCCAGGG CCCGGAGGCT GCTGGCCCCC 3181 TCTCCACTCC CCAAGAGGTT AGTGCTTCCC GGTCCTACCC AACTTTCTTG TACAAAGTGG 3241 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 3301 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 3361 ACCCCGGTGA GCGGCCGACG TAAATACGCG TTAAGTCgac aatcaacctc tggattacaa 3421 aatttgtgaa agatt