Construct: ORF TRCN0000472435
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003131.1_s317c1
- Derived from:
- ccsbBroadEn_01455
- DNA Barcode:
- GGACCGTGTACATGATTACCGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS16 (6217)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472435
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6217 | RPS16 | ribosomal protein S16 | NM_001020.6 | 100% | 100% | |
| 2 | human | 6217 | RPS16 | ribosomal protein S16 | NM_001321111.2 | 88.3% | 88.3% | 95_96ins51 |
| 3 | human | 6217 | RPS16 | ribosomal protein S16 | NM_001363860.2 | 67.7% | 56.6% | 247_341del;456_457ins77 |
| 4 | human | 7643 | ZNF90 | zinc finger protein 90 | XR_001753753.2 | 9.7% | (many diffs) | |
| 5 | mouse | 20055 | Rps16 | ribosomal protein S16 | NM_013647.2 | 89.4% | 100% | (many diffs) |
| 6 | mouse | 100862433 | LOC100862433 | 40S ribosomal protein S16 | XM_003688749.3 | 78.7% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 504
- ORF length:
- 438
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gtccaagggc ccgctgcagt ctgtgcaggT CTTCGGACGC AAGAAGACAG 121 CGACAGCTGT GGCGCACTGC AAACGCGGCA ATGGTCTCAT CAAGGTGAAC GGGCGGCCCC 181 TGGAGATGAT TGAGCCGCGC ACGCTACAGT ACAAGCTGCT GGAGCCAGTT CTGCTTCTCG 241 GCAAGGAGCG ATTTGCTGGT GTAGACATCC GTGTCCGTGT AAAGGGTGGT GGTCACGTGG 301 CCCAGATTTA TGCTATCCGT CAGTCCATCT CCAAAGCCCT GGTGGCCTAT TACCAGAAAT 361 ATGTGGATGA GGCTTCCAAG AAGGAGATCA AAGACATCCT CATCCAGTAT GACCGGACCC 421 TGCTGGTAGC TGACCCTCGT CGCTGCGAGT CCAAAAAGTT TGGAGGCCCT GGTGCCCGCG 481 CTCGCTACCA GAAATCCTAC CGATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 541 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 601 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG GACCGTGTAC 661 ATGATTACCG CAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 721 att