Construct: ORF TRCN0000472439
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015810.1_s317c1
- Derived from:
- ccsbBroadEn_01476
- DNA Barcode:
- ACATTTAGGTCCGAAAGTATATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- S100A8 (6279)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472439
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6279 | S100A8 | S100 calcium binding protei... | NM_001319201.1 | 100% | 100% | |
| 2 | human | 6279 | S100A8 | S100 calcium binding protei... | NM_002964.5 | 100% | 100% | |
| 3 | human | 6279 | S100A8 | S100 calcium binding protei... | NM_001319198.1 | 92% | 92% | 1_24del |
| 4 | human | 6279 | S100A8 | S100 calcium binding protei... | NM_001319197.1 | 80.1% | 80.1% | 1_69del |
| 5 | human | 6279 | S100A8 | S100 calcium binding protei... | NM_001319196.1 | 79.4% | 79.4% | 1_72del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 217
- ORF end:
- 496
- ORF length:
- 279
- Sequence:
-
1 GGTTTTATGC GATGGAGTTT CCCCACACTG AGTGGGTGGA GACTGAAGTT AGGCCAGCTT 61 GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA GTTTGGATCT TGGTTCATTC 121 TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGCTAGC 181 ATCGATTGAT CAACAAGTTT GTACAAAAAA GTTGGCATGT TGACCGAGCT GGAGAAAGCC 241 TTGAACTCTA TCATCGACGT CTACCACAAG TACTCCCTGA TAAAGGGGAA TTTCCATGCC 301 GTCTACAGGG ATGACCTGAA GAAATTGCTA GAGACCGAGT GTCCTCAGTA TATCAGGAAA 361 AAGGGTGCAG ACGTCTGGTT CAAAGAGTTG GATATCAACA CTGATGGTGC AGTTAACTTC 421 CAGGAGTTCC TCATTCTGGT GATAAAGATG GGCGTGGCAG CCCACAAAAA AAGCCATGAA 481 GAAAGCCACA AAGAGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC 541 CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGCCCGTA ACTTGAAAGT 601 ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AACATTTAGG TCCGAAAGTA 661 TATTCACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt