Construct: ORF TRCN0000472443
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004527.1_s317c1
- Derived from:
- ccsbBroadEn_10438
- DNA Barcode:
- GGTGAGATCTCTGTGTCTTCTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LGALS8 (3964)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472443
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3964 | LGALS8 | galectin 8 | NM_006499.4 | 98.8% | 98.3% | (many diffs) |
| 2 | human | 3964 | LGALS8 | galectin 8 | NM_201545.2 | 98.8% | 98.3% | (many diffs) |
| 3 | human | 3964 | LGALS8 | galectin 8 | XM_011544188.3 | 88.8% | 86% | (many diffs) |
| 4 | human | 3964 | LGALS8 | galectin 8 | NM_201543.3 | 87.1% | 86.6% | (many diffs) |
| 5 | human | 3964 | LGALS8 | galectin 8 | NM_201544.3 | 87.1% | 86.6% | (many diffs) |
| 6 | human | 3964 | LGALS8 | galectin 8 | XM_017001274.2 | 77.2% | 74.3% | (many diffs) |
| 7 | human | 3964 | LGALS8 | galectin 8 | XM_017001275.2 | 62.1% | 61.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1140
- ORF length:
- 1074
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt gtccttaaac aacctacaga atatcatcta taacccggta atcccgtatg 121 ttggcaccat tcccgatcag ctggatcctg gaactttgat tgtgatatgt gggcatgttc 181 ctagtgacgc agacagattc caggtggatc tgcagaatgg cagcagtgtg aaacctcgag 241 ccgatgtggc ctttcatttc aatcctcgtt tcaaaagggc cggctgcatt gtttgcaata 301 ctttgataaa tgaaaaatgg ggacgggaag agatcaccta tgacacgcct ttcaaaagag 361 aaaagtcttt tgagatcgtg attatggtgc taaaggacaa attccaggtg gctgtaaatg 421 gaaaacatac tctgctctat ggccacagga tcggcccaga gaaaatagac actctgggca 481 tttatggcaa agtgaatatt cactcaattg gttttagctt cagctcggac ttacaaagta 541 cccaagcatc tagtctggaa ctgacagaga taagtagaga aaatgttcca aagtctggca 601 cgccccagct tcctagtaat agaggaggag acatttctaa aaTCGCACCC AGAACTGTCT 661 ACACCAAGAG CAAAGATTCG ACTGTCAATC ACACTTTGAC TTGCACCAAA ATACCACCTA 721 TGAACTATGT GTCAAAGAGC CTGCCATTCG CTGCAAGGTT GAACACCCCC ATGGGCCCTG 781 GACGAACTGT CGTCGTTAAA GGAGAAGTGA ATGCAAATGC CAAAAGCTTT AATGTTGACC 841 TACTAGCAGG AAAATCAAAG GATATTGCTC TACACTTGAA CCCACGCCTG AATATTAAAG 901 CATTTGTAAG AAATTCTTTT CTTCAGGAGT CCTGGGGAGA AGAAGAGAGA AATATTACCT 961 CTTTCCCATT TAGTCCTGGG ATGTACTTTG AGATGATAAT TTACTGTGAT GTTAGAGAAT 1021 TCAAGGTTGC AGTAAATGGC GTACACAGCC TGGAGTACAA ACACAGATTT AAAGAGCTCA 1081 GCAGTATTGA CACGCTGGAA ATTAATGGAG ACATCCACTT ACTGGAAGTA TGGAGCTGGT 1141 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1201 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1261 TTTATATATC TTGTGGAAAG GACGAGGTGA GATCTCTGTG TCTTCTTATA CGCGTTAAGT 1321 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt