Construct: ORF TRCN0000472449
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005427.1_s317c1
- Derived from:
- ccsbBroadEn_12327
- DNA Barcode:
- TTCAGGAAAATAACCCAACGTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCNP (57092)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472449
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57092 | PCNP | PEST proteolytic signal con... | NM_001320395.1 | 100% | 100% | |
| 2 | human | 57092 | PCNP | PEST proteolytic signal con... | XM_017006922.1 | 99.2% | 99.2% | 279_280insAAG |
| 3 | human | 57092 | PCNP | PEST proteolytic signal con... | NM_001320400.1 | 80.7% | 80.7% | 278_279ins75 |
| 4 | human | 57092 | PCNP | PEST proteolytic signal con... | NM_020357.3 | 73% | 62.7% | 355_410del;447_534del |
| 5 | human | 57092 | PCNP | PEST proteolytic signal con... | NM_001320398.1 | 72.4% | 62.2% | 279_280insAAG;352_407del;444_531del |
| 6 | human | 57092 | PCNP | PEST proteolytic signal con... | NM_001320401.1 | 71.6% | 66% | (many diffs) |
| 7 | human | 57092 | PCNP | PEST proteolytic signal con... | NM_001320397.1 | 64.7% | 45.2% | (many diffs) |
| 8 | human | 57092 | PCNP | PEST proteolytic signal con... | NM_001320399.1 | 56.4% | 44.2% | (many diffs) |
| 9 | human | 57092 | PCNP | PEST proteolytic signal con... | NR_135232.1 | 15.1% | (many diffs) | |
| 10 | human | 57092 | PCNP | PEST proteolytic signal con... | NR_135227.1 | 15% | (many diffs) | |
| 11 | human | 57092 | PCNP | PEST proteolytic signal con... | NR_135228.1 | 14.5% | 1_122del;184_185ins52;461_2279del | |
| 12 | human | 57092 | PCNP | PEST proteolytic signal con... | NR_135223.1 | 14.1% | (many diffs) | |
| 13 | human | 57092 | PCNP | PEST proteolytic signal con... | NR_135225.1 | 14% | (many diffs) | |
| 14 | human | 57092 | PCNP | PEST proteolytic signal con... | NR_135230.1 | 12.7% | (many diffs) | |
| 15 | human | 57092 | PCNP | PEST proteolytic signal con... | NR_135226.1 | 11.2% | (many diffs) | |
| 16 | mouse | 76302 | Pcnp | PEST proteolytic signal con... | NM_001024622.2 | 67.9% | 58.5% | (many diffs) |
| 17 | mouse | 76302 | Pcnp | PEST proteolytic signal con... | XM_006522697.3 | 67.4% | 57.9% | (many diffs) |
| 18 | mouse | 76302 | Pcnp | PEST proteolytic signal con... | XM_011246041.2 | 52.3% | 41% | (many diffs) |
| 19 | mouse | 76302 | Pcnp | PEST proteolytic signal con... | XM_006522695.3 | 43.8% | 35% | (many diffs) |
| 20 | mouse | 76302 | Pcnp | PEST proteolytic signal con... | XM_006522696.3 | 43.4% | 34.6% | (many diffs) |
| 21 | mouse | 76302 | Pcnp | PEST proteolytic signal con... | XM_017317156.1 | 31.2% | 23.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 480
- ORF length:
- 390
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCGGACGG GAAGGCGGGA GACGAGAAGC 121 CTGAAAAGTC GCAGCGAGCT GGAGCCGCCG GAGGACCTGA AGAAGAAGCA GAAAAACCTG 181 TGAAAACTAA GACTGTTTCT TCCAGTAATG GAGGGGAAAG TTCCAGTCGC AGCGCTGAGA 241 AGCGATCAGC TGAAGAAGAA GCTGCCGACC TCCCAACAAA GCCTACAAAG ATCTCCAAGT 301 TTGGATTTGC CATAGGTAGT CAGACGACAA AGAAAGCATC AGCCATATCC ATCAAACTTG 361 GATCAAGTAA GCCTAAAGAA ACTGTTCCAA CTCTTGCTCC AAAAACTCTT TCAGTAGCAG 421 CAGCTTTTAA TGAAGATGAA GATGGATACA CCAACATCAG CTGGACCAAA CTCCTTCAAT 481 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 541 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 601 TTTATATATC TTGTGGAAAG GACGATTCAG GAAAATAACC CAACGTCCTA CGCGTTAAGT 661 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt