Construct: ORF TRCN0000472472
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011736.1_s317c1
- Derived from:
- ccsbBroadEn_11712
- DNA Barcode:
- TACTATGTGATGTCTAAATTAGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL18 (23276)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472472
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23276 | KLHL18 | kelch like family member 18 | XM_017006035.1 | 90.7% | 90.7% | 0_1ins141 |
| 2 | human | 23276 | KLHL18 | kelch like family member 18 | XM_017006034.1 | 89.8% | 89.8% | 0_1ins141;559_573del |
| 3 | human | 23276 | KLHL18 | kelch like family member 18 | XM_017006033.1 | 89% | 89% | 0_1ins141;559_573del;799_813del |
| 4 | human | 23276 | KLHL18 | kelch like family member 18 | NM_025010.5 | 88.6% | 88.6% | 1_195del |
| 5 | human | 23276 | KLHL18 | kelch like family member 18 | XM_005265002.5 | 87.9% | 87.9% | 1_195del;895_909del |
| 6 | human | 23276 | KLHL18 | kelch like family member 18 | XM_005265003.3 | 87.9% | 87.9% | 1_195del;1120_1134del |
| 7 | human | 23276 | KLHL18 | kelch like family member 18 | XM_005265001.3 | 87.1% | 87.1% | 1_195del;895_909del;1135_1149del |
| 8 | mouse | 270201 | Klhl18 | kelch-like 18 | NM_177771.5 | 80.5% | 87.1% | (many diffs) |
| 9 | mouse | 270201 | Klhl18 | kelch-like 18 | XM_006512141.3 | 79.8% | 86.3% | (many diffs) |
| 10 | mouse | 270201 | Klhl18 | kelch-like 18 | XM_017313405.1 | 51.1% | 55.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1593
- ORF length:
- 1527
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tacaaatgac atgatggagt gcaagcagga tgagattgta atgcaaggaa 121 tggacccaag tgccctggag gctctgatca actttgccta caacggcaac cttgccattg 181 accagcaaaa tgtccagtca ttgctgatgg gggcgagctt cctgcagctg cagagcatca 241 aagacgcctg ctgcacattc cttcgagaac ggcttcaccc aaaaaactgc ctgggtgtgc 301 gccagtttgc tgagacaatg atgtgtgctg tgctgtacga cgctgccaac agcttcatcc 361 accagcactt tgtggaggtg tccatgtcag aagagttcct ggccctgccc ttggaagacg 421 tgcttgagct ggtgtctcgg gatgagctga atgtcaaatc tgaggagcag gtctttgaag 481 ctgcattggc ctgggtcaga tacgaccggg agcagagggg tccctacctg cctgagctgc 541 tgtccaatat ccgcctgccc ctctgtcggc cccagttcct ttcagacaga gtacagcagg 601 atgacctggt gcgttgctgc cacaaatgca gggacctggt agacgaagca aaggactacc 661 acctcatgcc agagcgccgg ccccacctgc cagctttcag aacccggcca cgctgctgca 721 catccatcgc tggacttatc tacgctgtag ggggcctcaa ctcagcaggt gattccctga 781 atgtggtgga agtgttcgac cccattgcca attgctggga gagatgccgt cccatgacaa 841 cagcccgcag ccgcgttggc gtggctgtgg tgaacgggct tctctatgcc atcggaggat 901 atgacggcca gctacggctg agcactgtgg aggcctacaa cccggagaca gacacatgga 961 ccagagtggg gagcatgaat agcaagagaa gtgccatggg gacagtcgtg ctggatgggc 1021 agatctacgt ctgtgggggc tacgatggca actcttccct cagctccgtg gagacctact 1081 cacctGAGAC GGACAAATGG ACAGTGGTGA CCTCGATGAG CTCGAATCGC AGTGCTGCTG 1141 GGGTTACAGT CTTTGAGGGC AGGATATATG TGTCAGGCGG CCATGATGGT TTGCAGATCT 1201 TCAGCAGTGT GGAACACTAC AACCACCACA CAGCCACCTG GCACCCTGCA GCTGGCATGC 1261 TCAACAAGCG CTGCCGGCAC GGAGCCGCCT CCCTGGGGAG CAAGATGTTT GTCTGCGGGG 1321 GCTACGATGG CTCTGGCTTC CTCAGCATTG CCGAGATGTA CAGCTCTGTG GCAGACCAGT 1381 GGTGCCTGAT TGTCCCCATG CACACGCGCA GGAGCCGGGT CTCCCTGGTG GCCAGCTGTG 1441 GGCGCCTCTA CGCTGTTGGG GGCTACGACG GACAGTCAAA CCTAAGCTCA GTGGAGATGT 1501 ATGACCCAGA GACAGACTGC TGGACATTCA TGGCCCCCAT GGCGTGCCAT GAGGGAGGGG 1561 TCGGTGTGGG CTGCATCCCT CTCCTCACCA TCTACCCAAC TTTCTTGTAC AAAGTGGTTG 1621 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1681 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 1741 CTATGTGATG TCTAAATTAG GGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1801 ttgtgaaaga tt