Construct: ORF TRCN0000472505
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018814.3_s317c1
- Derived from:
- ccsbBroadEn_14954
- DNA Barcode:
- CCCGGTATCGCAATGGCCGGAACG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SGK2 (10110)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472505
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10110 | SGK2 | serum/glucocorticoid regula... | NM_001199264.1 | 99.6% | 35.2% | (many diffs) |
| 2 | human | 10110 | SGK2 | serum/glucocorticoid regula... | NM_170693.3 | 99.6% | 35.2% | (many diffs) |
| 3 | human | 10110 | SGK2 | serum/glucocorticoid regula... | NM_016276.3 | 85.6% | 30.3% | (many diffs) |
| 4 | mouse | 27219 | Sgk2 | serum/glucocorticoid regula... | NM_013731.3 | 88.7% | 33.3% | (many diffs) |
| 5 | mouse | 27219 | Sgk2 | serum/glucocorticoid regula... | XM_006499658.3 | 88.7% | 33.3% | (many diffs) |
| 6 | mouse | 27219 | Sgk2 | serum/glucocorticoid regula... | NM_001291152.1 | 88.5% | 33% | (many diffs) |
| 7 | mouse | 27219 | Sgk2 | serum/glucocorticoid regula... | NM_001291154.1 | 81.9% | 37.1% | (many diffs) |
| 8 | mouse | 27219 | Sgk2 | serum/glucocorticoid regula... | XM_011239580.2 | 81.9% | 37.1% | (many diffs) |
| 9 | mouse | 27219 | Sgk2 | serum/glucocorticoid regula... | XM_006499660.3 | 71.5% | 40.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 549
- ORF length:
- 480
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaactctagc ccagctggga ccccaagtcc acagccctcc agggccaatg 121 ggaacatcaa cctggggcct tcagccaacc caaatgccca gcccacggac ttcgacttcc 181 tcaaagtcat cggcaaaggg aactacggga aggtcctact ggccaagcgc aagtctgatg 241 gggcgttcta tgcagtgaag gtactacaga aaaagtccat cttaaagaag aaagagcaga 301 gccacatcat ggcagagcgc agtgtgcttc tgaagaacgt gcggcacccc ttcctcgtgg 361 gcctgcgcta ctccttccag acacctgaga agctctactt cgtgctcgac tatgtcaacg 421 gnggaggagc tcttcttcca cctgcagcgg gagcgccggt tcctggagcc ccgggcccag 481 gttctacgct gctgaggtgg ccagcgccat tggctacctg cactccctca acatcattta 541 cagggatctg aaaccagaga acattctctt ggactgccag ggacacgtgg tgctgacgga 601 ttttggcctc tgcaaggaag gtgtagagcc tgaagacacc acatccacat tctgtggtac 661 ccctgagtac ttggcacctg aagtgcttcg gaaagagcct tatgatcgag cagtggactg 721 gtggtgcttg ggggcagtcc tctacgagat gctccatggg CCTGCCGCCC TTCTACAGCC 781 AAGATGTATC CCAGATGTAT GAGAACATTC TGCACCAGCC GCTACAGATC CCCGGAGGCC 841 GGACAGTGGC CGCCTGTGAC CTCCTGCAAA GCCTTCTCCA CAAGGACCAG AGGCAGCGGC 901 TGGGCTCCAA AGCAGACTTT CTTGAGATTA AGAACCATGT ATTCTTCAGC CCCATAAACT 961 GGGATGACCT GTACCACAAG AGGCTAACTC CACCCTTCAA CCCAAATGTG ACAGGACCTG 1021 CTGACTTGAA GCATTTTGAC CCAGAGTTCA CCCAGGAAGC TGTGTCCAAG TCCATTGGCT 1081 GTACCCCTGA CACTGTGGCC AGCAGCTCTG GGGCCTCAAG TGCATTCCTG GGATTTTCTT 1141 ATGCGCCAGA GGATGATGAC ATCTTGGATT GCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1201 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1261 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 1321 CGGTATCGCA ATGGCCGGAA CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1381 ttgtgaaaga tt