Construct: ORF TRCN0000472541

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015270.1_s317c1
Derived from:
ccsbBroadEn_06613
DNA Barcode:
ATCGCGCAGATCGTGGAATATTAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PPP1R12A (4659)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472541
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4659 PPP1R12A protein phosphatase 1 regul... NM_001143885.1 99.9% 99.9% 4A>C
2 human 4659 PPP1R12A protein phosphatase 1 regul... NM_002480.3 99.9% 99.9% 4A>C
3 human 4659 PPP1R12A protein phosphatase 1 regul... NM_001244990.1 96.5% 96.5% 4A>C;2800_2801ins105
4 human 4659 PPP1R12A protein phosphatase 1 regul... NM_001244992.1 94.5% 94.4% 4A>C;1653_1654ins168
5 human 4659 PPP1R12A protein phosphatase 1 regul... NM_001143886.1 91.5% 91.5% 0_1ins261
6 mouse 17931 Ppp1r12a protein phosphatase 1, regu... XM_006513326.3 89% 93.5% (many diffs)
7 mouse 17931 Ppp1r12a protein phosphatase 1, regu... XM_006513327.3 85.3% 89.4% (many diffs)
8 mouse 17931 Ppp1r12a protein phosphatase 1, regu... NM_027892.2 84.8% 88.3% (many diffs)
9 mouse 17931 Ppp1r12a protein phosphatase 1, regu... XM_006513328.3 84% 88.8% (many diffs)
10 mouse 17931 Ppp1r12a protein phosphatase 1, regu... XM_006513329.3 83.8% 88.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3159
ORF length:
3090
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gcagatggcg gacgcgaagc agaagcggaa cgagcagctg aaacgctgga
121tcggctccga gacggacctc gagcctccgg tggtgaagcg ccagaagacc aaggtgaagt
181tcgacgatgg cgccgtcttc ctggctgctt gctccagcgg cgacacggac gaggtcctca
241agctgctgca ccgcggcgcc gacatcaatt acgccaatgt ggacggactc actgccctgc
301accaggcttg cattgatgac aatgttgata tggtgaagtt tctggtagaa aatggagcaa
361atattaatca acctgataat gaaggctgga taccactaca tgcagcagct tcctgtggat
421atcttgatat tgcagagttt ttgattggtc aaggagcaca tgtaggggct gtcaacagtg
481aaggagatac acctttagat attgcggagg aggaggcaat ggaagagcta cttcaaaatg
541aagttaatcg gcaaggggtt gatatagaag cagctcgaaa ggaagaagaa cggatcatgc
601ttagagatgc caggcagtgg ctaaatagtg gtcatataaa tgatgtccgg catgcaaaat
661ctggaggtac agcacttcac gttgcagctg ctaaaggcta tacggaagtt ttaaaacttt
721taatacaggc aggctatgat gttaatatta aagactatga tggctggaca cctcttcatg
781ctgcagctca ttggggtaaa gaagaagcat gtcgaatttt agtggacaat ctgtgtgata
841tggagatggt caacaaagtg ggccaaacag cctttgatgt agcagatgaa gacattttag
901gatatttaga agagttgcaa aagaaacaaa atctgctcca tagtgaaaaa cgggacaaga
961aatctccact aattgaatca acagcaaata tggacaataa tcagtcacag aagaccttta
1021aaaacaaaga gacgttgatt attgaaccag agaaaaatgc atcccgtatt gaatctctgg
1081aacaagaaaa ggttgatgaa gaagaagaag gaaagaagga tgagtctagc tgctctagtg
1141aagaagatga ggaagatgac tcggaatcag aagctgaaac agataagaca aaacccctgg
1201cttctgtaac taatgccaac acttctagta cacaagcagc tcctgtagct gttacaacac
1261ctactgtgtc atcaggtcaa gcaacaccta catcacctat taaaaagttt ccaaccacag
1321ctacaaaaat ttctcccaaa gaagaagaga gaaaagatga gtctcctgca acttggaggt
1381taggacttag aaagacgggc agctatggtg cacttgctga aatcacagca tctaaagagg
1441gtcagaaaga aaaagatact gcaggtgtta cacgttcagc ttcaagtccc agactttcct
1501cctctttgga taataaagaa aaggagaaag atagtaaagg aactaggctt gcatatgttg
1561cacctacaat accaagacga ctagccagta catctgacat tgaagagaaa gaaaacagag
1621attcttcaag tttgcgaaca agtagttcat atacaaggag aaaatgggaa gatgatctta
1681aaaaaaatag ctcagttaat gaaggatcaa cgtatcataa aagttgctcc tttggtagaa
1741gacaagatga tttgattagt tctagtgttc caagcaccac atcaacacca acagttacct
1801ctgcagctgg gcttcagaaa agcctgcttt ccagcacaag cactactaca aagattacaa
1861cgggttcttc ctcagcaggc acacaaagca gtacctcaaa tcgtttgtgg gctgaggata
1921gtactgagaa agaaaaggac agtgttccta cggcagtgac cattcctgtt gctccaactg
1981ttgtaaatgc tgcagcttct accacaaccc tgactacaac tactgctggc actgtctcct
2041ccacaacaga ggtcagggag agacgcagat catacctcac tcctgttagg gatgaagagt
2101ctgaatccca aagaaaagca agatctagac aagcaagaca atctagaaga tcaacacagg
2161gagtgacatt aactgatctt caagaagctg agaaaacaat aggaagaagt cgttctaccc
2221gaaccagaga acaagaaaat gaagaaaaag aaaaagagga aaaagagaaa caagataaag
2281agaaacaaga agaaaagaag gagtcagaaa catctagaga agatgaatat aaacaaaagt
2341actccagaac gtatgatgag acttaccagc gttataggcc agtatcaact tcaagttcaa
2401ccactccatc ctcttcactt tctactatga gcagttcact gtatgcttca agtcaactaa
2461acaggccaaa tagtcttgta ggcataactt ctgcttactc cagaggaata acaaaagaaa
2521atgaaagaga gggagaaaaa agagaagagg agaaagaagg agaagataaa tcacaaccta
2581aatcaatcag agaacgacga cgaccaagag agaaaagaag atctacagga gtttcatttt
2641ggacacaaga tagtgatgaa aatgaacaag aacaacaaTC AGACACAGAA GAGGGATCCA
2701ATAAGAAAGA AACTCAGACG GATTCCATTT CTAGATATGA AACCAGTTCT ACATCAGCTG
2761GTGATCGATA TGATTCCTTG CTGGGTCGCT CTGGATCATA CAGTTACTTA GAAGAAAGAA
2821AACCTTACAG CAGCAGGCTA GAAAAGGATG ACTCAACTGA CTTTAAAAAG CTTTATGAAC
2881AAATTCTAGC TGAAAATGAA AAGCTGAAGG CACAGCTACA TGATACAAAT ATGGAACTAA
2941CAGATCTTAA ATTACAGTTG GAAAAGGCCA CCCAGAGACA AGAAAGATTT GCTGATAGAT
3001CACTGTTGGA AATGGAAAAA AGGGAACGAA GAGCTCTAGA AAGAAGAATA TCTGAAATGG
3061AAGAAGAGCT CAAAATGTTA CCAGACCTAA AAGCAGACAA CCAGAGGCTA AAGGATGAAA
3121ATGGGGCCTT GATCAGAGTT ATAAGCAAAC TTTCCAAATT GCCAACTTTC TTGTACAAAG
3181TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
3241GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
3301ACGAATCGCG CAGATCGTGG AATATTAGAC GCGTTAAGTC gacaatcaac ctctggatta
3361caaaatttgt gaaagatt

Download FASTA (ORF) (Full)