Construct: ORF TRCN0000472541
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015270.1_s317c1
- Derived from:
- ccsbBroadEn_06613
- DNA Barcode:
- ATCGCGCAGATCGTGGAATATTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP1R12A (4659)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472541
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4659 | PPP1R12A | protein phosphatase 1 regul... | NM_001143885.1 | 99.9% | 99.9% | 4A>C |
| 2 | human | 4659 | PPP1R12A | protein phosphatase 1 regul... | NM_002480.3 | 99.9% | 99.9% | 4A>C |
| 3 | human | 4659 | PPP1R12A | protein phosphatase 1 regul... | NM_001244990.1 | 96.5% | 96.5% | 4A>C;2800_2801ins105 |
| 4 | human | 4659 | PPP1R12A | protein phosphatase 1 regul... | NM_001244992.1 | 94.5% | 94.4% | 4A>C;1653_1654ins168 |
| 5 | human | 4659 | PPP1R12A | protein phosphatase 1 regul... | NM_001143886.1 | 91.5% | 91.5% | 0_1ins261 |
| 6 | mouse | 17931 | Ppp1r12a | protein phosphatase 1, regu... | XM_006513326.3 | 89% | 93.5% | (many diffs) |
| 7 | mouse | 17931 | Ppp1r12a | protein phosphatase 1, regu... | XM_006513327.3 | 85.3% | 89.4% | (many diffs) |
| 8 | mouse | 17931 | Ppp1r12a | protein phosphatase 1, regu... | NM_027892.2 | 84.8% | 88.3% | (many diffs) |
| 9 | mouse | 17931 | Ppp1r12a | protein phosphatase 1, regu... | XM_006513328.3 | 84% | 88.8% | (many diffs) |
| 10 | mouse | 17931 | Ppp1r12a | protein phosphatase 1, regu... | XM_006513329.3 | 83.8% | 88.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3159
- ORF length:
- 3090
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagatggcg gacgcgaagc agaagcggaa cgagcagctg aaacgctgga 121 tcggctccga gacggacctc gagcctccgg tggtgaagcg ccagaagacc aaggtgaagt 181 tcgacgatgg cgccgtcttc ctggctgctt gctccagcgg cgacacggac gaggtcctca 241 agctgctgca ccgcggcgcc gacatcaatt acgccaatgt ggacggactc actgccctgc 301 accaggcttg cattgatgac aatgttgata tggtgaagtt tctggtagaa aatggagcaa 361 atattaatca acctgataat gaaggctgga taccactaca tgcagcagct tcctgtggat 421 atcttgatat tgcagagttt ttgattggtc aaggagcaca tgtaggggct gtcaacagtg 481 aaggagatac acctttagat attgcggagg aggaggcaat ggaagagcta cttcaaaatg 541 aagttaatcg gcaaggggtt gatatagaag cagctcgaaa ggaagaagaa cggatcatgc 601 ttagagatgc caggcagtgg ctaaatagtg gtcatataaa tgatgtccgg catgcaaaat 661 ctggaggtac agcacttcac gttgcagctg ctaaaggcta tacggaagtt ttaaaacttt 721 taatacaggc aggctatgat gttaatatta aagactatga tggctggaca cctcttcatg 781 ctgcagctca ttggggtaaa gaagaagcat gtcgaatttt agtggacaat ctgtgtgata 841 tggagatggt caacaaagtg ggccaaacag cctttgatgt agcagatgaa gacattttag 901 gatatttaga agagttgcaa aagaaacaaa atctgctcca tagtgaaaaa cgggacaaga 961 aatctccact aattgaatca acagcaaata tggacaataa tcagtcacag aagaccttta 1021 aaaacaaaga gacgttgatt attgaaccag agaaaaatgc atcccgtatt gaatctctgg 1081 aacaagaaaa ggttgatgaa gaagaagaag gaaagaagga tgagtctagc tgctctagtg 1141 aagaagatga ggaagatgac tcggaatcag aagctgaaac agataagaca aaacccctgg 1201 cttctgtaac taatgccaac acttctagta cacaagcagc tcctgtagct gttacaacac 1261 ctactgtgtc atcaggtcaa gcaacaccta catcacctat taaaaagttt ccaaccacag 1321 ctacaaaaat ttctcccaaa gaagaagaga gaaaagatga gtctcctgca acttggaggt 1381 taggacttag aaagacgggc agctatggtg cacttgctga aatcacagca tctaaagagg 1441 gtcagaaaga aaaagatact gcaggtgtta cacgttcagc ttcaagtccc agactttcct 1501 cctctttgga taataaagaa aaggagaaag atagtaaagg aactaggctt gcatatgttg 1561 cacctacaat accaagacga ctagccagta catctgacat tgaagagaaa gaaaacagag 1621 attcttcaag tttgcgaaca agtagttcat atacaaggag aaaatgggaa gatgatctta 1681 aaaaaaatag ctcagttaat gaaggatcaa cgtatcataa aagttgctcc tttggtagaa 1741 gacaagatga tttgattagt tctagtgttc caagcaccac atcaacacca acagttacct 1801 ctgcagctgg gcttcagaaa agcctgcttt ccagcacaag cactactaca aagattacaa 1861 cgggttcttc ctcagcaggc acacaaagca gtacctcaaa tcgtttgtgg gctgaggata 1921 gtactgagaa agaaaaggac agtgttccta cggcagtgac cattcctgtt gctccaactg 1981 ttgtaaatgc tgcagcttct accacaaccc tgactacaac tactgctggc actgtctcct 2041 ccacaacaga ggtcagggag agacgcagat catacctcac tcctgttagg gatgaagagt 2101 ctgaatccca aagaaaagca agatctagac aagcaagaca atctagaaga tcaacacagg 2161 gagtgacatt aactgatctt caagaagctg agaaaacaat aggaagaagt cgttctaccc 2221 gaaccagaga acaagaaaat gaagaaaaag aaaaagagga aaaagagaaa caagataaag 2281 agaaacaaga agaaaagaag gagtcagaaa catctagaga agatgaatat aaacaaaagt 2341 actccagaac gtatgatgag acttaccagc gttataggcc agtatcaact tcaagttcaa 2401 ccactccatc ctcttcactt tctactatga gcagttcact gtatgcttca agtcaactaa 2461 acaggccaaa tagtcttgta ggcataactt ctgcttactc cagaggaata acaaaagaaa 2521 atgaaagaga gggagaaaaa agagaagagg agaaagaagg agaagataaa tcacaaccta 2581 aatcaatcag agaacgacga cgaccaagag agaaaagaag atctacagga gtttcatttt 2641 ggacacaaga tagtgatgaa aatgaacaag aacaacaaTC AGACACAGAA GAGGGATCCA 2701 ATAAGAAAGA AACTCAGACG GATTCCATTT CTAGATATGA AACCAGTTCT ACATCAGCTG 2761 GTGATCGATA TGATTCCTTG CTGGGTCGCT CTGGATCATA CAGTTACTTA GAAGAAAGAA 2821 AACCTTACAG CAGCAGGCTA GAAAAGGATG ACTCAACTGA CTTTAAAAAG CTTTATGAAC 2881 AAATTCTAGC TGAAAATGAA AAGCTGAAGG CACAGCTACA TGATACAAAT ATGGAACTAA 2941 CAGATCTTAA ATTACAGTTG GAAAAGGCCA CCCAGAGACA AGAAAGATTT GCTGATAGAT 3001 CACTGTTGGA AATGGAAAAA AGGGAACGAA GAGCTCTAGA AAGAAGAATA TCTGAAATGG 3061 AAGAAGAGCT CAAAATGTTA CCAGACCTAA AAGCAGACAA CCAGAGGCTA AAGGATGAAA 3121 ATGGGGCCTT GATCAGAGTT ATAAGCAAAC TTTCCAAATT GCCAACTTTC TTGTACAAAG 3181 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 3241 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 3301 ACGAATCGCG CAGATCGTGG AATATTAGAC GCGTTAAGTC gacaatcaac ctctggatta 3361 caaaatttgt gaaagatt