Construct: ORF TRCN0000472558
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008274.1_s317c1
- Derived from:
- ccsbBroadEn_08302
- DNA Barcode:
- TTTCACGACAACCCCGTATTTTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTF2 (51507)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472558
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51507 | RTF2 | replication termination fac... | NM_016407.4 | 99.8% | 99.6% | 511A>G |
| 2 | human | 51507 | RTF2 | replication termination fac... | NM_001283036.1 | 99.5% | 99.3% | 511A>G;642_643insGAA |
| 3 | human | 51507 | RTF2 | replication termination fac... | XM_017027872.1 | 93.3% | 92.1% | (many diffs) |
| 4 | human | 51507 | RTF2 | replication termination fac... | NM_001283035.1 | 90.9% | 90.7% | 70_159del;601A>G |
| 5 | human | 51507 | RTF2 | replication termination fac... | NM_001283037.1 | 75% | 66.6% | 511A>G;588_589ins55;690_691ins173 |
| 6 | mouse | 66404 | Rtf2 | replication termination fac... | NM_025542.2 | 84.4% | 88.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 984
- ORF length:
- 918
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ttgcgacggg ggaacaatcc ccaagaggca tgaactggtg aaggggccga 121 agaaggttga gaaggtcgac aaagatgctg aattagtggc ccaatggaac tattgtactc 181 taagtcagga aatattaaga cgaccaatag ttgcctgtga acttggcaga ctttataaca 241 aagatgccgt cattgaattt ctcttggaca aatctgcaga aaaggctctt gggaaggcag 301 catctcacat taaaagcatt aagaatgtga cagagctgaa gctttctgat aatcctgcct 361 gggaagggga taaaggaaac actaaaggtg acaagcacga tgacctccag cgggcgcgtt 421 tcatctgccc cgttgtgggc ctggagatga acggccgaca caggttctgc ttccttcggt 481 gctgcggctg tgtgttttct gagcgagcct tgaaagagat aaaagcggaa gtttgccaca 541 cgtgtggggc tgCCTTCCAG GAGGATGATG TCATCGTGCT CAATGGCACC AAGGAGGATG 601 TGGACGTGCT GAAGACAAGG ATGGAGGAGA GAAGGCTGAG AGCGAAGCTG GAAAAGAAAA 661 CAAAGAAACC CAAGGCAGCA GAGTCTGTTT CAAAACCAGA TGTCAGTGAA GAAGCCCCAG 721 GGCCATCAAA AGTTAAGACA GGGAAGCCTG AAGAAGCCAG CCTTGATTCT AGAGAGAAGA 781 AAACCAACTT GGCTCCCAAA AGCACAGCAA TGAATGAGAG CTCTTCTGGA AAAGCTGGGA 841 AGCCTCCGTG TGGAGCCACA AAGAGGTCCA TCGCTGACAG TGAAGAATCG GAGGCCTACA 901 AGTCCCTCTT TACCACTCAC AGCTCCGCCA AGCGCTCCAA GGAGGAGTCT GCCCACTGGG 961 TCACCCACAC GTCCTACTGC TTCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1021 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1081 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TTCACGACAA 1141 CCCCGTATTT TAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1201 att