Construct: ORF TRCN0000472559

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016207.1_s317c1
Derived from:
ccsbBroadEn_11812
DNA Barcode:
TTATTCTGCGCAGCTCAGATTGTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LDLRAP1 (26119)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472559
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26119 LDLRAP1 low density lipoprotein rec... XM_024446315.1 99.7% 99.6% 469T>C;519A>G
2 human 26119 LDLRAP1 low density lipoprotein rec... XM_017000994.2 93.3% 93.2% 1_54del;523T>C;573A>G
3 human 26119 LDLRAP1 low density lipoprotein rec... NM_015627.3 85.1% 85% 1_135del;604T>C;654A>G
4 human 26119 LDLRAP1 low density lipoprotein rec... XM_011541209.3 84.8% 84.7% (many diffs)
5 human 26119 LDLRAP1 low density lipoprotein rec... XM_006710559.4 72.4% 70% (many diffs)
6 human 26119 LDLRAP1 low density lipoprotein rec... XM_011541210.3 72.1% 69.7% (many diffs)
7 human 26119 LDLRAP1 low density lipoprotein rec... XM_006710560.4 71.5% 69.8% (many diffs)
8 human 26119 LDLRAP1 low density lipoprotein rec... XM_011541211.3 71.2% 69.4% (many diffs)
9 human 26119 LDLRAP1 low density lipoprotein rec... XM_006710561.4 70.7% 70.1% (many diffs)
10 human 26119 LDLRAP1 low density lipoprotein rec... XM_011541212.3 70.4% 69.8% (many diffs)
11 human 26119 LDLRAP1 low density lipoprotein rec... XR_946602.3 58.7% (many diffs)
12 human 26119 LDLRAP1 low density lipoprotein rec... XR_001737112.2 55.3% (many diffs)
13 human 26119 LDLRAP1 low density lipoprotein rec... XM_017000995.2 35.6% 35% (many diffs)
14 human 26119 LDLRAP1 low density lipoprotein rec... XR_001737113.2 26.2% (many diffs)
15 human 26119 LDLRAP1 low density lipoprotein rec... XR_426598.4 26.1% (many diffs)
16 human 26119 LDLRAP1 low density lipoprotein rec... XR_002956258.1 20.3% (many diffs)
17 human 26119 LDLRAP1 low density lipoprotein rec... XR_946603.3 17.2% (many diffs)
18 mouse 100017 Ldlrap1 low density lipoprotein rec... XM_006538423.3 84.9% 87.8% (many diffs)
19 mouse 100017 Ldlrap1 low density lipoprotein rec... NM_145554.2 72.8% 75.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
855
ORF length:
789
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgct gttcagcctc aagtacctgg gcatgacgct agtggagcag cccaagggtg
121aggagctgtc ggccgccgcc atcaagagga tcgtggctac agctaaggcc agtgggaaga
181agctgcagaa ggtgactctg aaggtgtcgc cacggggaat tatcctgaca gacaacctca
241ccaaccagct cattgagaac gtgtccatat acaggatctc ctattgcaca gcagacaaga
301tgcacgacaa ggtgtttgca tacatcgccc agagccagca caaccagagc ctcgagtgcc
361acgccttcct ctgcaccaag cggaagatgg cacaggctgt taccctcacc GTAGCCCAGG
421CCTTCAAAGT CGCCTTTGAG TTTTGGCAGG TGTCCAAGGA AGAGAAAGAG AAGAGGGACA
481AAGCCAGCCA AGAGGGAGGG GACGTCCTGG GGGCCCGCCA AGACTGCACC CCCCCCTTGA
541AGAGCTTGGT CGCCACTGGG AACCTGCTGG ACTTAGAGGA GACGGCTAAG GCCCCGCTGT
601CCACGGTCAG CGCCAACACC ACCAACATGG ACGAGGTGCC GCGGCCACAA GCCTTGAGTG
661GCAGCAGTGT TGTCTGGGAG CTGGATGATG GCCTGGATGA AGCGTTTTCG AGGCTTGCCC
721AGTCTCGGAC AAACCCTCAG GTCCTGGACA CTGGCCTGAC AGCCCAGGAC ATGCATTACG
781CCCAGTGCCT CTCGCCTGTC GACTGGGACA AGCCTGACAG CAGCGGCACA GAGCAGGATG
841ACCTCTTCAG CTTCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
901CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
961TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TTATTCTGCG CAGCTCAGAT
1021TGTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)