Construct: ORF TRCN0000472625
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009523.1_s317c1
- Derived from:
- ccsbBroadEn_10649
- DNA Barcode:
- TAGCACACACGCGCCGGTTGATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A51P4 (494141)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472625
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92014 | SLC25A51 | solute carrier family 25 me... | NM_033412.4 | 45% | 33% | (many diffs) |
| 2 | human | 147407 | SLC25A52 | solute carrier family 25 me... | NM_001034172.4 | 44.1% | 29.7% | (many diffs) |
| 3 | human | 494141 | SLC25A51P4 | SLC25A51 pseudogene 4 | NR_026564.1 | 26.5% | 1_238del;411C>T;701_1739del | |
| 4 | human | 442229 | SLC25A51P1 | SLC25A51 pseudogene 1 | NR_026540.1 | 26.2% | (many diffs) | |
| 5 | human | 494141 | SLC25A51P4 | SLC25A51 pseudogene 4 | NR_026563.1 | 24.4% | 1_383del;556C>T;846_1884del | |
| 6 | human | 494141 | SLC25A51P4 | SLC25A51 pseudogene 4 | NR_026541.1 | 22% | 1_238del;411C>T;701_2093del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 79
- ORF end:
- 541
- ORF length:
- 462
- Sequence:
-
1 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT 61 TTGTACAAAA AAGTTGGCAT GAAAAAAGAA GAACTTAAGC AACATGATGG ATTCAGAAGC 121 TCATGGAAAG AGACCACCAA TACTAACATC TTCGAAACAA GATATGTCAC CTCATATTAC 181 AGATTTAGTG AAATGAAGCA TTACTTGTGT GGCTGCTGTG CAGCCTTCAA CAACGTCGCA 241 ATCACATTTC TCATTCAGAA GGTCCTCTTT CCACAACAGC TGTATGGCAT CAAAACGGGG 301 GATGCAATAC TTCAGTTGAG AACGGATGGA TTTCGAAACT TGTATCGGGG AATCTTTCCC 361 CGATTGATGC AGAAGACAAC TACACTTGCA CTTACGTTTG GTCTGTATGA GGATTTATCC 421 TACCTTCTCC ACAAGCATGT CAGTGCTCCA GAGTTTGCAA CCTGTGGCGT GGCAGCAGTG 481 CTTGCAGGGA CAACGGAAGC AATTTTCACT TCAGACATTG CTTCAAGACC ACAAGCACCA 541 TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC 601 GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG 661 CTTTATATAT CTTGTGGAAA GGACGATAGC ACACACGCGC CGGTTGATAT ACGCGTTAAG 721 TCgacaatca acctctggat tacaaaattt gtgaaagatt