Construct: ORF TRCN0000472631
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018176.1_s317c1
- Derived from:
- ccsbBroadEn_06816
- DNA Barcode:
- CGCGTTCTGTTACATAAATGCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTPRA (5786)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472631
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5786 | PTPRA | protein tyrosine phosphatas... | NM_080840.2 | 99.9% | 100% | 882C>T |
| 2 | human | 5786 | PTPRA | protein tyrosine phosphatas... | NM_080841.2 | 99.9% | 100% | 882C>T |
| 3 | human | 5786 | PTPRA | protein tyrosine phosphatas... | NM_002836.3 | 98.8% | 98.8% | 414_440del;909C>T |
| 4 | mouse | 19262 | Ptpra | protein tyrosine phosphatas... | NM_001163688.1 | 90.6% | 95.9% | (many diffs) |
| 5 | mouse | 19262 | Ptpra | protein tyrosine phosphatas... | XM_006498989.3 | 89.7% | 94.8% | (many diffs) |
| 6 | mouse | 19262 | Ptpra | protein tyrosine phosphatas... | NM_008980.2 | 86.7% | 91.6% | (many diffs) |
| 7 | mouse | 19262 | Ptpra | protein tyrosine phosphatas... | XM_006498990.3 | 80.7% | 84.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2445
- ORF length:
- 2379
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ttcctggttc attcttgttc tgctcggcag tggtctgata tgtgtcagtg 121 ccaacaatgc taccacagtt gcaccttctg taggaattac aagattaatt aactcatcaa 181 cggcagaacc agttaaagaa gaggccaaaa cttcaaatcc aacttcttca ctaacttctc 241 tttctgtggc accaacattc agcccaaata taactctggg acccacctat ttaaccactg 301 tcaattcttc agactctgac aatgggacca caagaacagc aagcaccaat tctataggca 361 ttacaatttc accaaatgga acgtggcttc cagataacca gttcacggat gccagaacag 421 aaccctggga ggggaattcc agcaccgcag caaccactcc agaaactttc cctccttcag 481 atgagacacc aattattgcg gtgatggtgg ccctgtcctc tctgctagtg atcgtgttta 541 ttatcatagt tttgtacatg ttaaggttta agaaatacaa gcaagctggg agccattcca 601 attctttccg cttatccaac ggccgcactg aggatgtgga gccccagagt gtgccacttc 661 tggccagatc cccaagcacc aacaggaaat acccacccct gcccgtggac aagctggaag 721 aggaaattaa ccggagaatg gcagacgaca ataagctctt cagggaggaa ttcaacgctc 781 tccctgcatg tcctatccag gccacctgtg aggctgcttc caaggaggaa aacaaggaaa 841 aaaatcgata tgtaaacatc ttgccttatg accactctag agtccacctg acaccggttg 901 aaggggttcc agattctgat tacatcaatg cttcattcat caacggttac caagaaaaga 961 acaaattcat tgctgcacaa ggaccaaaag aagaaacggt gaatgatttc tggcggatga 1021 tctgggaaca aaacacagcc accatcgtca tggttaccaa cctgaaggag agaaaggagt 1081 gcaagtgcgc ccagtactgg ccagaccaag gctgctggac ctatgggaat attcgggtgt 1141 ctgtagagga tgtgactgtc ctggtggact acacagtacg gaagttctgc atccagcagg 1201 tgggcgacat gaccaacaga aagccacagc gcctcatcac tcagttccac tttaccagct 1261 ggccagactt tggggtgcct tttaccccga tcggcatgct caagttcctc aagaaggtga 1321 aggcctgtaa ccctcagtat gcaggggcca tcgtggtcca ctgcagtgca ggtgtagggc 1381 gtacaggtac ctttgtcgtc attgatgcca tgctggacat gatgcataca gaacggaagg 1441 tggacgtgta tggctttgtg agccggatcc gggcacagcg ctgccagatg gtgcaaaccg 1501 atatgcagta tgtcttcata taccaagccc ttctggagca ttatctctat ggagatacag 1561 aactggaagt gacctctcta gaaacccacc tgcagaaaat ttacaacaaa atcccaggga 1621 ccagcaacaa tggattagag gaggagttta agaagttaac atcaatcaaa atccagaatg 1681 acaagatgcg gactggaaac cttccagcca acatgaagaa gaaccgtgtt ttacagatca 1741 ttccatatga attcaacaga gtgatcattc cagttaagcg gggcgaagag aatacagact 1801 atgtgaacgc atcctttatt gatggctacc ggcagaagga ctcctatatc gccagccagg 1861 gccctcttct ccacacaatt gaggacttct ggcgaatgat ctgggagtgg aaatcctgct 1921 ctatcgtgat gctaacagaa ctggaggaga gaggccagga gaagtgtgcc cagtactggc 1981 catcTGATGG ACTGGTGTCC TATGGAGATA TTACAGTGGA ACTGAAGAAG GAGGAGGAAT 2041 GTGAGAGCTA CACCGTCCGA GACCTCCTGG TCACCAACAC CAGGGAGAAT AAGAGCCGGC 2101 AGATCCGGCA GTTCCACTTC CATGGCTGGC CTGAAGTGGG CATCCCCAGT GACGGAAAGG 2161 GCATGATCAG CATCATCGCC GCCGTGCAGA AGCAGCAGCA GCAGTCAGGG AACCACCCCA 2221 TCACCGTGCA CTGCAGCGCC GGGGCAGGAA GGACGGGGAC CTTCTGTGCC CTGAGCACCG 2281 TCCTGGAGCG TGTGAAAGCA GAGGGGATTT TGGATGTCTT CCAGACTGTC AAGAGCCTGC 2341 GGCTACAGAG GCCACACATG GTCCAGACAC TGGAACAGTA TGAGTTCTGC TACAAGGTGG 2401 TGCAGGAGTA TATTGATGCA TTCTCAGATT ATGCCAACTT CAAGTACCCA ACTTTCTTGT 2461 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2521 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2581 GAAAGGACGA CGCGTTCTGT TACATAAATG CCCCACGCGT TAAGTCgaca atcaacctct 2641 ggattacaaa atttgtgaaa gatt