Construct: ORF TRCN0000472659

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001440.1_s317c1
Derived from:
ccsbBroadEn_03358
DNA Barcode:
TCGAGCATATCACAATCCAGGTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MPC1 (51660)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472659
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51660 MPC1 mitochondrial pyruvate carr... NM_016098.4 100% 100%
2 human 51660 MPC1 mitochondrial pyruvate carr... NM_001270879.2 60.5% 60.5% 0_1ins129
3 human 51660 MPC1 mitochondrial pyruvate carr... XM_011535895.2 60.5% 60.5% 0_1ins129
4 human 51660 MPC1 mitochondrial pyruvate carr... XM_024446456.1 60.5% 60.5% 0_1ins129
5 human 51660 MPC1 mitochondrial pyruvate carr... NR_073093.2 28.8% 1_64del;140_368del;621_1133del
6 mouse 55951 Mpc1 mitochondrial pyruvate carr... NM_018819.4 90.2% 94.4% (many diffs)
7 mouse 55951 Mpc1 mitochondrial pyruvate carr... XM_011246219.2 71.3% 71.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
157
ORF end:
484
ORF length:
327
Sequence:
1GGCACTTGAT GTAATTCTCC TTGGAATTTG CCCTTTTTGA GTTTGGATCT TGGTTCATTC
61TCAAGCCTCA GACAGTGGTT CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGCTAGC
121ATCGATTGAT CAACAAGTTT GTACAAAAAA GTTGGCATGG CGGGCGCGTT GGTGCGGAAA
181GCGGCGGACT ATGTCCGAAG CAAGGATTTC CGGGACTACC TCATGAGTAC GCACTTCTGG
241GGCCCAGTAG CCAACTGGGG TCTTCCCATT GCTGCCATCA ATGATATGAA AAAGTCTCCA
301GAGATTATCA GTGGGCGGAT GACATTTGCC CTCTGTTGCT ATTCTTTGAC ATTCATGAGA
361TTTGCCTACA AGGTACAGCC TCGGAACTGG CTTCTGTTTG CATGCCACGC AACAAATGAA
421GTAGCCCAGC TCATCCAGGG AGGGCGGCTT ATCAAACACG AGATGACTAA AACGGCATCT
481GCATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC
541CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT
601TGGCTTTATA TATCTTGTGG AAAGGACGAT CGAGCATATC ACAATCCAGG TTTACGCGTT
661AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)

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