Construct: ORF TRCN0000472667
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018875.2_s317c1
- Derived from:
- ccsbBroadEn_15037
- DNA Barcode:
- AACACTAAATACATGGCAACCTCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SRPK3 (26576)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472667
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26576 | SRPK3 | SRSF protein kinase 3 | NM_001170760.2 | 98.1% | 41.1% | (many diffs) |
| 2 | human | 26576 | SRPK3 | SRSF protein kinase 3 | NM_014370.4 | 97.9% | 41% | (many diffs) |
| 3 | human | 26576 | SRPK3 | SRSF protein kinase 3 | NM_001170761.1 | 92.5% | 43.7% | (many diffs) |
| 4 | mouse | 56504 | Srpk3 | serine/arginine-rich protei... | NM_019684.2 | 84.3% | 38.5% | (many diffs) |
| 5 | mouse | 56504 | Srpk3 | serine/arginine-rich protei... | XM_006528172.3 | 80.7% | 36.8% | (many diffs) |
| 6 | mouse | 56504 | Srpk3 | serine/arginine-rich protei... | XM_017318579.1 | 37.4% | 75.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 810
- ORF length:
- 741
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcgccagc acgggcggtg gtggggacag cggcggcagc ggcggcagta 121 gcagcagctc acaggcctcc tgcgggcccg agtcctcggg ctccgaacta gccctggcca 181 caccggtgcc tcagatgctg cagggccttc tgggctccga cgacgaggaa caggaagacc 241 ccaaagacta ctgcaagggc ggctaccacc ctgtgaagat cggcgacgtg ttcaatgggc 301 ggtaccacgt ggtgcgcaaa ctgggctggg gccacttctc caccgtctgg ctctgctggg 361 acatccagcg caagcgcttt gtggccctca aagtggtgaa gagtgcgggg cattacacgg 421 agacagctgt ggatgagatc aagctcctga aatgtgtccg ggacagcgac cccagtgacc 481 ccaaaagaga gaccattgtc cagctcattg atgacttcag gatctcagga gtcaatggag 541 tccatgtgtg catggtgctg gaggtgctgg gccaccagct cctcaaatgg atcatcaagt 601 ccaactacca gggcctgccc gtgccctgcg tgaagagcat cgtgaggcag gtgctgcacg 661 gcctggacta cctccacacc aagtgcaaga tcatccacac ggacatcaag cccgagaaca 721 tcttgctgtg tgtgggggac gcttacatca gcgcctggct gccgaggcca cggagtgcaa 781 caggcagggg cgccgccccc tcccgctcat agtcagcact gccccccaga gtcttgaccg 841 gtaagctgtc caaaaacaag aggaagaaga tgaggcgcaa cgggaaacag cagaagcggc 901 tgctggagga gcggctgcgg gacctgcaga ggctggaggc catggaaggt gtcccacccc 961 agggcgaagg attctggctt gggaattgga cgggggcagg ccggctccac atcttccttc 1021 aggctgtcac ccccgggggc gccagagcca gtccctcccc aagcctcttc ctcccccngc 1081 cccagggggc ggccgtagcc tcagcgcggg ctcacagacc tcaggcttct ccggctccct 1141 cttctctcct gcctcctgct ccatcctctc cggctcgtcc aatcagcgag agaccggggg 1201 cctcctgtcg cctagcacac cattcggtgc ctcgaacctc ctggtgaacc ccctggagcc 1261 ccaaaatgca gataagatca agatcaagat cgcagacctg ggcaacgcct gctgggtgca 1321 caagcacttc acggaagaca tccagactcg gcagtaccgg gccgtcgagg tgctgatcgg 1381 cgccgaatac ggccccccgg cagacatctg gagcacagcc tgcatggccT TCGAGCTGGC 1441 CACTGGTGAC TACCTGTTCG AGCCGCATTC TGGAGAAGAC TACAGTCGTG ATGAGGACCA 1501 CATCGCTCAC ATAGTGGAGC TTCTGGGGGA CATCCCCCCA GCCTTCGCCC TCTCAGGCCG 1561 CTATTCCCGG GAGTTCTTCA ACCGGAGAGG AGAGCTGCGG CACATCCACA ATCTCAAGCA 1621 CTGGGGCCTG TACGAGGTAC TCATGGAAAA GTACGAGTGG CCCCTAGAGC AGGCCACACA 1681 GTTCAGCGCC TTTCTGCTGC CCATGATGGA GTACATCCCC GAAAAGCGGG CCAGTGCCGC 1741 TGACTGCCTC CAGCACCCCT GGCTCAACCC CTTGCCAACT TTCTTGTACA AAGTGGTTGA 1801 TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG 1861 TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAC 1921 ACTAAATACA TGGCAACCTC AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 1981 tgtgaaagat t