Construct: ORF TRCN0000472671
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006026.1_s317c1
- Derived from:
- ccsbBroadEn_01536
- DNA Barcode:
- CTCCGTTCCATTCTTGTAGTAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SIM2 (6493)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472671
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6493 | SIM2 | SIM bHLH transcription fact... | NM_009586.5 | 100% | 100% | |
| 2 | human | 6493 | SIM2 | SIM bHLH transcription fact... | NM_005069.6 | 83% | 77.8% | (many diffs) |
| 3 | human | 6493 | SIM2 | SIM bHLH transcription fact... | XM_011529694.1 | 68% | 63% | (many diffs) |
| 4 | human | 6493 | SIM2 | SIM bHLH transcription fact... | XM_017028442.2 | 57.3% | 57% | 974_975ins23;981_982ins706 |
| 5 | human | 6493 | SIM2 | SIM bHLH transcription fact... | XR_001754891.2 | 47.2% | 1_630del;1479_1480ins148;1736_1737ins456 | |
| 6 | mouse | 20465 | Sim2 | single-minded homolog 2 (Dr... | XM_017316897.1 | 83.7% | 85.7% | (many diffs) |
| 7 | mouse | 20465 | Sim2 | single-minded homolog 2 (Dr... | NM_011377.2 | 74.5% | 74.6% | (many diffs) |
| 8 | mouse | 20465 | Sim2 | single-minded homolog 2 (Dr... | XM_006522966.3 | 74.1% | 74.1% | (many diffs) |
| 9 | mouse | 20465 | Sim2 | single-minded homolog 2 (Dr... | XM_006522967.3 | 60.7% | 59.8% | (many diffs) |
| 10 | mouse | 20465 | Sim2 | single-minded homolog 2 (Dr... | XM_006522968.3 | 60.7% | 59.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1779
- ORF length:
- 1710
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaggagaag tccaagaatg cggccaagac caggagggag aaggaaaatg 121 gcgagtttta cgagcttgcc aagctgctcc cgctgccgtc ggccatcact tcgcagctgg 181 acaaagcgtc catcatccgc ctcaccacga gctacctgaa gatgcgcgcc gtcttccccg 241 aaggtttagg agacgcgtgg ggacagccga gccgcgccgg gcccctggac ggcgtcgcca 301 aggagctggg atcgcacttg ctgcagactt tggatggatt tgtttttgtg gtagcatctg 361 atggcaaaat catgtatata tccgagaccg cttctgtcca tttaggctta tcccaggtgg 421 agctcacggg caacagtatt tatgaataca tccatccttc tgaccacgat gagatgaccg 481 ctgtcctcac ggcccaccag ccgctgcacc accacctgct ccaagagtat gagatagaga 541 ggtcgttctt tcttcgaatg aaatgtgtct tggcgaaaag gaacgcgggc ctgacctgca 601 gcggatacaa ggtcatccac tgcagtggct acttgaagat caggcagtat atgctggaca 661 tgtccctgta cgactcctgc taccagattg tggggctggt ggccgtgggc cagtcgctgc 721 cacccagtgc catcaccgag atcaagctgt acagtaacat gttcatgttc agggccagcc 781 ttgacctgaa gctgatattc ctggattcca gggtgaccga ggtgacgggg tacgagccgc 841 aggacctgat cgagaagacc ctataccatc acgtgcacgg ctgcgacgtg ttccacctcc 901 gctacgcaca ccacctcctg ttggtgaagg gccaggtcac caccaagtac taccggctgc 961 tgtccaagcg gggcggctgg gtgtgggtgc agagctacgc caccgtggtg cacaacagcc 1021 gctcgtcccg gccccactgc atcgtgagtg tcaattatgt actcacggag attgaataca 1081 aggaacttca gctgtccctg gagcaggtgt ccactgccaa gtcccaggac tcctggagga 1141 ccgccttgtc tacctcacaa gaaactagga aattagtgaa acccaaaaat accaagatga 1201 agacaaagct gagaacaaac ccttaccccc cacagcaata cagctcgttc caaatggaca 1261 aactggaatg cggccagctc ggaaactgga gagccagtcc ccctgcaagc gctgctgctc 1321 ctccagaact gcagccccac tcagaaagca gtgaccttct gtacacgcca tcctacagcc 1381 tgcccTTCTC CTACCATTAC GGACACTTCC CTCTGGACTC TCACGTCTTC AGCAGCAAAA 1441 AGCCAATGTT GCCGGCCAAG TTCGGGCAGC CCCAAGGATC CCCTTGTGAG GTGGCACGCT 1501 TTTTCCTGAG CACACTGCCA GCCAGCGGTG AATGCCAGTG GCATTATGCC AACCCCCTAG 1561 TGCCTAGCAG CTCGTCTCCA GCTAAAAATC CTCCAGAGCC ACCGGCGAAC ACTGCTAGGC 1621 ACAGCCTGGT GCCAAGCTAC GAAGGTGGGT CAGGTCTGCT CGTGGGGAAG GTGGGAGGAC 1681 TGCGCACGGC CGGGAGCCGA AGCAGCCATG GCGGTGGGTG GCAGATGGAG ACAGAACCCT 1741 CACGCTTTGG GCAAACTTGC CCTCTTTCTG CTTCTAAGTT GCCAACTTTC TTGTACAAAG 1801 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1861 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1921 ACGACTCCGT TCCATTCTTG TAGTAACTAC GCGTTAAGTC gacaatcaac ctctggatta 1981 caaaatttgt gaaagatt