Transcript: Human XR_001754891.2

PREDICTED: Homo sapiens SIM bHLH transcription factor 2 (SIM2), transcript variant X3, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SIM2 (6493)
Length:
1736
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001754891.2
NBCI Gene record:
SIM2 (6493)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001754891.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000015152 CGGGCAACAGTATTTATGAAT pLKO.1 989 3UTR 100% 5.625 7.875 N SIM2 n/a
2 TRCN0000015151 GCCTTGTCTACCTCACAAGAA pLKO.1 1557 3UTR 100% 0.495 0.693 N SIM2 n/a
3 TRCN0000429100 ACGACTCCTGCTACCAGATTG pLKO_005 1232 3UTR 100% 10.800 7.560 N SIM2 n/a
4 TRCN0000422875 AGATAGAGAGGTCGTTCTTTC pLKO_005 1094 3UTR 100% 10.800 7.560 N SIM2 n/a
5 TRCN0000015149 CCTGATCGAGAAGACCCTATA pLKO.1 1407 3UTR 100% 10.800 7.560 N SIM2 n/a
6 TRCN0000015150 GCTGCAGACTTTGGATGGATT pLKO.1 882 3UTR 100% 4.950 3.465 N SIM2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001754891.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01536 pDONR223 100% 47.2% None 1_630del;1479_1480ins148;1736_1737ins456 n/a
2 ccsbBroad304_01536 pLX_304 0% 47.2% V5 1_630del;1479_1480ins148;1736_1737ins456 n/a
3 TRCN0000472671 CTCCGTTCCATTCTTGTAGTAACT pLX_317 23.3% 47.2% V5 1_630del;1479_1480ins148;1736_1737ins456 n/a
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