Construct: ORF TRCN0000472683
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006515.1_s317c1
- Derived from:
- ccsbBroadEn_10691
- DNA Barcode:
- CTCGCCTAGTACTGAAGGCTCTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472683
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 527 | ATP6V0C | ATPase H+ transporting V0 s... | NM_001198569.1 | 72.2% | 72.2% | 1_129del |
| 2 | human | 527 | ATP6V0C | ATPase H+ transporting V0 s... | NM_001694.4 | 72.2% | 72.2% | 1_129del |
| 3 | mouse | 11984 | Atp6v0c | ATPase, H+ transporting, ly... | NM_009729.3 | 63.6% | 69% | (many diffs) |
| 4 | mouse | 100039636 | Atp6v0c-ps2 | ATPase, H+ transporting, ly... | NR_037854.1 | 26.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 426
- ORF length:
- 336
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCTGTCAT GCGGCCGGAG CAGATCATGA 121 AGTCCATCAT CCCAGTGGTC ATGGCTGGCA TCATCGCCAT CTACGGCCTG GTGGTGGCAG 181 TCCTCATCGC CAACTCCCTG AATGACGACA TCAGCCTCTA CAAGAGCTTC CTCCAGCTGG 241 GCGCCGGCCT GAGCGTGGGC CTGAGCGGCC TGGCAGCCGG CTTTGCCATC GGCATCGTGG 301 GGGACGCTGG CGTGCGGGGC ACCGCCCAGC AGCCCCGACT ATTCGTGGGC ATGATCCTGA 361 TTCTCATCTT CGCCGAGGTG CTCGGCCTCT ACGGTCTCAT CGTCGCCCTC ATCCTCTCCA 421 CAAAGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 481 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 541 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTCGCCTAG TACTGAAGGC TCTGCACGCG 601 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt