Construct: ORF TRCN0000472693
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001527.1_s317c1
- Derived from:
- ccsbBroadEn_08417
- DNA Barcode:
- CAGAGCCGTTCCCTCGTGCTATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ESRP1 (54845)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472693
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54845 | ESRP1 | epithelial splicing regulat... | NM_017697.4 | 99.9% | 100% | 984C>T;1800T>C |
| 2 | human | 54845 | ESRP1 | epithelial splicing regulat... | NM_001034915.3 | 99.3% | 99.4% | 984C>T;1635_1636insCCATGTAAGTTA;1788T>C |
| 3 | human | 54845 | ESRP1 | epithelial splicing regulat... | XM_005250991.3 | 96.8% | 96.4% | (many diffs) |
| 4 | human | 54845 | ESRP1 | epithelial splicing regulat... | NM_001122826.2 | 96.2% | 95.8% | (many diffs) |
| 5 | human | 54845 | ESRP1 | epithelial splicing regulat... | XM_005250992.3 | 91.7% | 90.1% | (many diffs) |
| 6 | human | 54845 | ESRP1 | epithelial splicing regulat... | NM_001122825.2 | 89.1% | 89.1% | (many diffs) |
| 7 | human | 54845 | ESRP1 | epithelial splicing regulat... | NM_001122827.2 | 88.5% | 88.5% | (many diffs) |
| 8 | mouse | 207920 | Esrp1 | epithelial splicing regulat... | NM_194055.3 | 90.7% | 97.2% | (many diffs) |
| 9 | mouse | 207920 | Esrp1 | epithelial splicing regulat... | NM_001290383.1 | 90.6% | 97% | (many diffs) |
| 10 | mouse | 207920 | Esrp1 | epithelial splicing regulat... | XM_006537715.1 | 90.1% | 96.6% | (many diffs) |
| 11 | mouse | 207920 | Esrp1 | epithelial splicing regulat... | XM_006537716.1 | 87.7% | 93.8% | (many diffs) |
| 12 | mouse | 207920 | Esrp1 | epithelial splicing regulat... | XM_006537717.1 | 82.9% | 87.6% | (many diffs) |
| 13 | mouse | 207920 | Esrp1 | epithelial splicing regulat... | XM_006537718.1 | 80.2% | 86.6% | (many diffs) |
| 14 | mouse | 207920 | Esrp1 | epithelial splicing regulat... | NM_001347052.1 | 79.6% | 86% | (many diffs) |
| 15 | mouse | 207920 | Esrp1 | epithelial splicing regulat... | XM_006537721.3 | 65.2% | 69.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2112
- ORF length:
- 2043
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacggcctct ccggattact tggtggtgct ttttgggatc actgctgggg 121 ccaccggggc caagctaggc tcggatgaga aggagttgat cctgctgttc tggaaagtcg 181 tggatctggc caacaagaag gtgggacagt tgcacgaagt gctagttaga ccggatcagt 241 tggaactgac ggaggactgc aaagaagaaa ctaaaataga cgtcgaaagc ctgtcctcgg 301 cgtcgcagct ggaccaagcc ctccgacagt ttaaccagtc agtgagcaat gaactgaata 361 ttggagtagg gacttccttc tgtctctgta ctgatgggca gcttcatgtc aggcaaatcc 421 tgcatcctga ggcttccaag aagaatgtac tattacctga atgcttctat tccttttttg 481 atcttcgaaa agaattcaag aaatgttgcc ctggttcacc tgatattgac aaactggacg 541 ttgccacaat gacagagtat ttaaattttg agaagagtag ttcagtctct cgatatggag 601 cctctcaagt tgaagatatg gggaatataa ttttagcaat gatttcagag ccttataatc 661 acaggttttc agatccagag agagtgaatt acaagtttga aagtggaact tgcagcaaga 721 tggaacttat tgatgataac accgtagtca gggcacgagg tttaccatgg cagtcttcag 781 atcaagatat tgcaagattc ttcaaaggac tcaatattgc caagggaggt gcagcacttt 841 gtctgaatgc tcagggtcga aggaacggag aagctctggt taggtttgta agtgaggagc 901 accgagacct agcactacag aggcacaaac atcacatggg gacccggtat attgaggttt 961 acaaagcaac aggtgaagat ttccttaaaa ttgctggtgg tacttccaat gaggtagccc 1021 agtttctctc caaggaaaat caagtcattg ttcgcatgcg ggggctccct ttcacggcca 1081 cagctgaaga agtggtggcc ttctttggac agcattgccc tattactggg ggaaaggaag 1141 gcatcctctt tgtcacctac ccagatggta ggccaacagg ggacgctttt gtcctctttg 1201 cctgtgagga atatgcacag aatgcgttga ggaagcataa agacttgttg ggtaaaagat 1261 acattgaact cttcaggagc acagcagctg aagttcagca ggtgctgaat cgattctcct 1321 cggcccctct cattccactt ccaacccctc ccattattcc agtactacct cagcaatttg 1381 tgccccctac aaatgttaga gactgtatac gccttcgagg tcttccctat gcagccacaa 1441 ttgaggacat cctggatttc ctgggggagt tcgccacaga tattcgtact catggggttc 1501 acatggtttt gaatcaccag ggccgcccat caggagatgc ctttatccag atgaagtctg 1561 cggacagagc atttatggct gcacagaagt gtcataaaaa aaacatgaag gacagatatg 1621 ttgaagTCTT TCAGTGTTCA GCTGAGGAGA TGAACTTTGT GTTAATGGGG GGCACTTTAA 1681 ATCGAAATGG CTTATCCCCA CCGCCATGTA AGTTACCATG CCTGTCTCCT CCCTCCTACA 1741 CATTTCCAGC TCCTGCTGCA GTTATTCCTA CAGAAGCTGC CATTTACCAG CCCTCTGTGA 1801 TTTTGAATCC ACGAGCACTG CAGCCCTCCA CAGCGTACTA CCCAGCAGGC ACTCAGCTCT 1861 TCATGAACTA CACAGCGTAC TATCCCAGCC CCCCAGGTTC GCCTAATAGT CTTGGCTACT 1921 TCCCTACAGC TGCTAATCTT AGCGGTGTCC CTCCACAGCC TGGCACGGTG GTCAGAATGC 1981 AGGGCCTGGC CTACAATACT GGAGTTAAGG AAATTCTTAA CTTCTTCCAA GGTTACCAGT 2041 ATGCAACCGA GGATGGACTT ATACACACAA ATGACCAGGC CAGGACTCTA CCCAAAGAAT 2101 GGGTTTGTAT TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2161 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2221 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAG AGCCGTTCCC TCGTGCTATT 2281 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t