Construct: ORF TRCN0000472716
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007189.1_s317c1
- Derived from:
- ccsbBroadEn_05249
- DNA Barcode:
- CCAAAGGTAGAGCTAAGACCTGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPP38-DT (221060)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472716
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221060 | RPP38-DT | RPP38 divergent transcript | NR_160789.1 | 27.1% | 1_274del;740_1714del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 531
- ORF length:
- 465
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga atccctgcag actccccagc accgcgaaaa tcaagataaa agggagaagg 121 agtatggggt aaaacacatg cctatgggCA ATAATGCAGG GAATCTTGAG CCCGAAAAGA 181 GAAAGGCAGT AAGAGTTGCC TTGAGTTCAG CAACAGCTGC ACAGAATATC CCGTCCAGTG 241 TCCACTGTGG CTGCTCCAAG CAATGGAGAC TCAGGCTACC ATCGGAGTCG CTGCAAAGTC 301 GGGGACAAGT GATGAAGCGG CCGAATAACA TTTTAAAGCT CAGGAATCTG GATCTGTTGA 361 TCTACCCTTG GCCAGAACTT AGAAGACGGC AGGTTGCTTC TGACCTAATG AGCCTCCTCC 421 TTCTCCCCGC TTTTTCCGGC CTTACTTGGG CCCCCTTCCT TTTCCTCTTT ACGTATCTGC 481 CTCCTTTTCT CAATCTCCTC ACTGTTGGTT TTGTATCCTA TTTTCTGGTA TACCCAACTT 541 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 601 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAC 661 TTGTGGAAAG GACGACCAAA GGTAGAGCTA AGACCTGTGA CGCGTTAAGT Cgacaatcaa 721 cctctggatt acaaaatttg tgaaagatt