Construct: ORF TRCN0000472726
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005402.1_s317c1
- Derived from:
- ccsbBroadEn_11826
- DNA Barcode:
- CCCACCGGGGCCGAACTTAGATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTPN18 (26469)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472726
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26469 | PTPN18 | protein tyrosine phosphatas... | NM_001142370.2 | 99.4% | 99.4% | 792_797delGACGGG |
| 2 | human | 26469 | PTPN18 | protein tyrosine phosphatas... | XM_006712416.4 | 98.3% | 98.3% | 792_797delGACGGG;1060_1071del |
| 3 | human | 26469 | PTPN18 | protein tyrosine phosphatas... | XM_017003798.1 | 80.3% | 80.3% | 0_1ins192;600_605delGACGGG;868_879del |
| 4 | human | 26469 | PTPN18 | protein tyrosine phosphatas... | NM_014369.4 | 76.3% | 76.3% | 94_414del;1113_1118delGACGGG |
| 5 | human | 26469 | PTPN18 | protein tyrosine phosphatas... | XM_006712417.1 | 75.6% | 75.6% | 94_414del;1113_1118delGACGGG;1381_1392del |
| 6 | human | 26469 | PTPN18 | protein tyrosine phosphatas... | XM_024452789.1 | 70.2% | 70.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1119
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ccgcagcctg gactcggcgc ggagcttcct ggagcggctg gaagcgcggg 121 gcggccggga gggggcagtc ctcgccggcg agttcagcaa aaggtgtgag cggtactggg 181 cccaggagca ggagccactg cagactgggc ttttctgcat cactctgata aaggagaagt 241 ggctgaatga ggacatcatg ctcaggaccc tcaaggtcac attccagaag gagtcccgtt 301 ctgtgtacca gctacagtat atgtcctggc cagaccgtgg ggtccccagc agtcctgacc 361 acatgctcgc catggtggag gaagcccgtc gcctccaggg atctggccct gaacccctct 421 gtgtccactg cagtgcgggt tgtgggcgaa caggcgtcct gtgcaccgtg gattatgtga 481 ggcagctgct cctgacccag atgatcccac ctgacttcag tctctttgat gtggtcctta 541 agatgaggaa gcagcggcct gcggccgtgc agacagagga gcagtacagg ttcctgtacc 601 acacggtggc tcagatgttc tgctccacac tccagaatgc cagcccccac taccagaaca 661 tcaaagagaa ttgtgcccca ctctacgacg atgccctctt cctccggact ccccaggcac 721 ttctcgccat accccgccca ccaggagggg tcctcaggag catctctgtg cccgggtccc 781 cgggccacgc catggctgac acctacgcgg tggtgcagaa gcgcggggct ccagcgggcg 841 ccgggagtgg gacgcagacg gggacgggga cgggggcgcg cagcgcggag gaggcgccgc 901 tcTACAGCAA GGTGACGCCG CGCGCCCAGC GACCCGGGGC GCACGCGGAG GACGCGAGGG 961 GGACGCTGCC TGGCCGCGTT CCTGCTGACC AAAGTCCTGC CGGATCTGGC GCCTACGAGG 1021 ACGTGGCGGG TGGAGCTCAG ACCGGTGGGC TAGGTTTCAA CCTGCGCATT GGGAGGCCGA 1081 AGGGTCCCCG GGACCCGCCT GCTGAGTGGA CCCGGGTGTA CCCAACTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGACCCACC GGGGCCGAAC TTAGATCCAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt