Transcript: Human NM_014369.4

Homo sapiens protein tyrosine phosphatase non-receptor type 18 (PTPN18), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-02
Taxon:
Homo sapiens (human)
Gene:
PTPN18 (26469)
Length:
3616
CDS:
53..1435

Additional Resources:

NCBI RefSeq record:
NM_014369.4
NBCI Gene record:
PTPN18 (26469)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_014369.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000381001 TGATGGCCTGTCGAGAGATAG pLKO_005 435 CDS 100% 10.800 15.120 N PTPN18 n/a
2 TRCN0000381010 GTCCTGTGCACCGTGGATTAT pLKO_005 764 CDS 100% 13.200 10.560 N PTPN18 n/a
3 TRCN0000380807 AGGACCCTCAAGGTCACATTC pLKO_005 572 CDS 100% 10.800 7.560 N PTPN18 n/a
4 TRCN0000380581 CAGAACTAAGCCAGGCATAAC pLKO_005 1842 3UTR 100% 10.800 7.560 N PTPN18 n/a
5 TRCN0000382258 CTCTTTGATGTGGTCCTTAAG pLKO_005 830 CDS 100% 10.800 7.560 N PTPN18 n/a
6 TRCN0000381490 GGAGGAGGTAGCTAGGGTATA pLKO_005 1611 3UTR 100% 10.800 7.560 N PTPN18 n/a
7 TRCN0000380069 TAAAGGAGAAGTGGCTGAATG pLKO_005 537 CDS 100% 10.800 7.560 N PTPN18 n/a
8 TRCN0000003045 GCCTGTCGAGAGATAGAGAAT pLKO.1 440 CDS 100% 4.950 3.465 N PTPN18 n/a
9 TRCN0000003044 GAGATAGAGAATGGGCGGAAA pLKO.1 449 CDS 100% 4.050 2.835 N PTPN18 n/a
10 TRCN0000003046 ACCAGAACATCAAAGAGAATT pLKO.1 960 CDS 100% 0.000 0.000 N PTPN18 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_014369.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11826 pDONR223 100% 76.3% 76.3% None 94_414del;1113_1118delGACGGG n/a
2 ccsbBroad304_11826 pLX_304 0% 76.3% 76.3% V5 94_414del;1113_1118delGACGGG n/a
3 TRCN0000472726 CCCACCGGGGCCGAACTTAGATCC pLX_317 21.1% 76.3% 76.3% V5 94_414del;1113_1118delGACGGG n/a
4 TRCN0000491725 TCATACATTCGTGTCTCGCGCAGC pLX_317 30.4% 76.3% 76.3% V5 (not translated due to prior stop codon) 94_414del;1113_1118delGACGGG n/a
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