Construct: ORF TRCN0000472745
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011208.1_s317c1
- Derived from:
- ccsbBroadEn_04707
- DNA Barcode:
- CTGCCTTCCTTTAATCGCCCGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LYPD1 (116372)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472745
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116372 | LYPD1 | LY6/PLAUR domain containing 1 | NM_144586.7 | 100% | 100% | |
| 2 | human | 116372 | LYPD1 | LY6/PLAUR domain containing 1 | NM_001321234.2 | 89.1% | 87.2% | (many diffs) |
| 3 | human | 116372 | LYPD1 | LY6/PLAUR domain containing 1 | NM_001077427.4 | 63.1% | 63.1% | 0_1ins156 |
| 4 | human | 116372 | LYPD1 | LY6/PLAUR domain containing 1 | NM_001321235.2 | 63.1% | 63.1% | 0_1ins156 |
| 5 | human | 116372 | LYPD1 | LY6/PLAUR domain containing 1 | XM_011510546.3 | 53.9% | 53.9% | 0_1ins195 |
| 6 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | NM_145100.4 | 89.5% | 93.6% | (many diffs) |
| 7 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | XM_017312443.1 | 80% | 80.8% | (many diffs) |
| 8 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | NM_001311089.1 | 56% | 58.1% | (many diffs) |
| 9 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | NM_001311090.1 | 56% | 58.1% | (many diffs) |
| 10 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | XM_006529885.3 | 56% | 58.1% | (many diffs) |
| 11 | mouse | 72585 | Lypd1 | Ly6/Plaur domain containing 1 | XM_011248088.2 | 43.3% | 49.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 513
- ORF length:
- 423
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTCGGCA TGTGGGTCCT AGGCATCGCG GCAACTTTTT 121 GCGGATTGTT CTTGCTTCCA GGCTTTGCGC TGCAAATCCA GTGCTACCAG TGTGAAGAAT 181 TCCAGCTGAA CAACGACTGC TCCTCCCCCG AGTTCATTGT GAATTGCACG GTGAACGTTC 241 AAGACATGTG TCAGAAAGAA GTGATGGAGC AAAGTGCCGG GATCATGTAC CGCAAGTCCT 301 GTGCATCATC AGCGGCCTGT CTCATCGCCT CTGCCGGGTA CCAGTCCTTC TGCTCCCCAG 361 GGAAACTGAA CTCAGTTTGC ATCAGCTGCT GCAACACCCC TCTTTGTAAC GGGCCAAGGC 421 CCAAGAAAAG GGGAAGTTCT GCCTCGGCCC TCAGGCCAGG GCTCCGCACC ACCATCCTGT 481 TCCTCAAATT AGCCCTCTTC TCGGCACACT GCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 541 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 601 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT 661 GCCTTCCTTT AATCGCCCGG GAACGCGTTA AGTCgacaat caacctctgg attacaaaat 721 ttgtgaaaga tt