Construct: ORF TRCN0000472764
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013735.1_s317c1
- Derived from:
- ccsbBroadEn_08390
- DNA Barcode:
- GGGACTAAAAAAGCTGTCGCCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTRF1L (54516)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472764
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54516 | MTRF1L | mitochondrial translational... | NM_019041.7 | 99.8% | 99.4% | 112A>G;227G>A |
| 2 | human | 54516 | MTRF1L | mitochondrial translational... | NM_001301870.2 | 90.3% | 90% | 112A>G;227G>A;413_414ins108 |
| 3 | human | 54516 | MTRF1L | mitochondrial translational... | NM_001114184.3 | 70.9% | 70% | (many diffs) |
| 4 | human | 54516 | MTRF1L | mitochondrial translational... | NM_001301871.2 | 62.6% | 62.6% | 0_1ins426 |
| 5 | human | 54516 | MTRF1L | mitochondrial translational... | NM_001301047.3 | 61.4% | 60.5% | (many diffs) |
| 6 | human | 54516 | MTRF1L | mitochondrial translational... | XR_245540.4 | 43.1% | (many diffs) | |
| 7 | human | 54516 | MTRF1L | mitochondrial translational... | NM_001301872.1 | 30.9% | 29.2% | (many diffs) |
| 8 | human | 54516 | MTRF1L | mitochondrial translational... | XR_001743474.2 | 29.3% | (many diffs) | |
| 9 | human | 54516 | MTRF1L | mitochondrial translational... | NR_126056.2 | 26.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1206
- ORF length:
- 1140
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttagcatgcg gtcccgggtt ctgtggggcg ctgcccggtg gctctggccc cgccgggccg 121 ttggcccagc ccgccggccc ctgagctccg gtagcccgcc gctggaggag ctgttcgccc 181 ggggcgggcc cttgcggacc ttcctcgagc gccaggcggg gtctgaagcc catttgaagg 241 tcaggaggcc cgagttgctg gcggtgatca aactgctgaa cgagaaggag caggagctgc 301 gggagactga gcacttgctg cacgatgaga atgaagattt aaggaaactt gcagagaatg 361 aaatcacttt gtgtcaaaaa gaaataactc agctgaagca tcagattatc ttacttttgg 421 ttccctcaga agaaacagat gaaaatgatt tgatcctgga agtaactgca ggagttggag 481 gtcaggaggc aatgttgttt acatcagaga tatttgatat gtatcagcaa tatgctgcat 541 ttaaaagatg gcattttgaa accctggaat attttccaag tgaactaggt ggccttagac 601 atgcatctgc cagcattggg ggttcagaag cctataggca catgaaattt gaaggaggtg 661 ttcacagagt acaaagagtg ccaaagacag aaaagcaagg ccgcgtccat actagcacca 721 tgactgtagc aatattaccc cagcctactg agattaatct ggtgattaat ccgaaagatt 781 tgagaattga cactaagcga gccagtggAG CTGGGGGGCA GCATGTAAAT ACCACGGACA 841 GTGCTGTCCG GATAGTTCAT CTTCCAACAG GTGTTGTTTC TGAATGTCAA CAAGAGAGAT 901 CTCAGCTGAA AAATAAAGAG CTGGCTATGA CAAAGTTACG TGCAAAACTG TACAGCATGC 961 ATCTAGAAGA AGAAATAAAT AAAAGACAGA ATGCTAGAAA AATTCAGATT GGAAGTAAAG 1021 GAAGATCAGA GAAAATAAGA ACATATAATT TTCCACAGAA CCGGGTCACA GATCACAGAA 1081 TAAACAAGAC GCTGCATGAT CTTGAAACTT TTATGCAAGG AGATTATCTA CTGGATGAAC 1141 TTGTACAGTC ATTGAAGGAA TACGCCGATT ATGAATCTTT AGTAGAAATT ATTTCCCAAA 1201 AAGTTTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1261 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1321 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGGGACTAAA AAAGCTGTCG CCACCACGCG 1381 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt