Construct: ORF TRCN0000472774
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008700.1_s317c1
- Derived from:
- ccsbBroadEn_03665
- DNA Barcode:
- GATTACGCAACGGCCTTGCCCTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ITFG2 (55846)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472774
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | NM_018463.4 | 100% | 100% | |
| 2 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XM_005253707.5 | 95% | 91.8% | (many diffs) |
| 3 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XM_024449057.1 | 73.7% | 69.4% | (many diffs) |
| 4 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XR_001748811.2 | 72.9% | 1_98del;1440_1839del | |
| 5 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XR_002957358.1 | 61% | 1_98del;1440_2195del | |
| 6 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XM_005253709.4 | 60.4% | 60.4% | 0_1ins531 |
| 7 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XR_002957357.1 | 57% | 1_98del;1440_2349del | |
| 8 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | NR_147202.2 | 56.4% | 1_93del;476_477ins22;1413_2316del | |
| 9 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XM_017019641.1 | 55.8% | 53% | (many diffs) |
| 10 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XR_001748810.2 | 54.6% | 1_98del;1440_2456del | |
| 11 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | NR_130744.3 | 50.1% | 1_93del;499_574del;1511_2674del | |
| 12 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XR_002957356.1 | 47.6% | 1_98del;1440_2815del | |
| 13 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XR_002957355.1 | 46.7% | 1_98del;1440_2867del | |
| 14 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XR_002957354.1 | 45.3% | 1_98del;1440_2960del | |
| 15 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XR_002957352.1 | 43% | 1_98del;1440_3114del | |
| 16 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XR_001748812.1 | 40.8% | (many diffs) | |
| 17 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XR_002957351.1 | 38.4% | 1_98del;1440_3484del | |
| 18 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XR_002957359.1 | 37.7% | 1_98del;1318_1387del;1510_3554del | |
| 19 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XR_002957360.1 | 37.4% | 1_117del;523_598del;1535_3579del | |
| 20 | human | 55846 | ITFG2 | integrin alpha FG-GAP repea... | XR_002957353.1 | 37.3% | 1_98del;1440_3591del | |
| 21 | mouse | 101142 | Itfg2 | integrin alpha FG-GAP repea... | NM_133927.1 | 86.5% | 89.4% | (many diffs) |
| 22 | mouse | 101142 | Itfg2 | integrin alpha FG-GAP repea... | XM_006505229.3 | 52.1% | 52.5% | (many diffs) |
| 23 | mouse | 101142 | Itfg2 | integrin alpha FG-GAP repea... | XM_006505230.2 | 52.1% | 52.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1407
- ORF length:
- 1341
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gtcagttagc tacgtgcagc gcgtggcgct ggagttcagc gggagcctct 121 tcccgcacgc aatctgcctc ggagacgttg ataacgatac gttaaatgaa ctggtggtgg 181 gagacaccag cgggaaggtg tctgtgtata aaaatgatga cagtcggcca tggctcacct 241 gttcctgcca gggaatgctg acttgcgttg gggttggaga cgtgtgtaat aaaggaaaga 301 acctgttggt ggcagtgagt gctgaaggct ggtttcattt gtttgacctg acacctgcca 361 aggtgttgga tgcttctggg caccacgaga cactaatcgg agaggagcag cgtccagtct 421 tcaagcagca catccctgcc aacaccaagg tcatgctgat cagcgacatc gatggagatg 481 ggtgtcgtga gctggtggtg ggctacacag accgtgtggt gcgagctttc cgctgggagg 541 agctaggtga gggtcctgaa catctgacag ggcagctggt gtccctcaag aaatggatgc 601 tggagggtca ggtggacagc ctctcagtga ctctggggcc actgggtctt cctgaactga 661 tggtgtctca gccaggttgt gcgtatgcaa ttctactgtg tacctggaaa aaggacactg 721 ggtcccctcc tgcctctgaa gggcccacgg atggtagtag ggagacccca gctgcccgag 781 acgtggtgct gcaccagaca tctggccgta tccacaacaa gaatgtctcc actcacctaa 841 ttggcaacat caaacaaggc cacggcactg agagtagtgg ctctggcctc tttgCCCTGT 901 GCACCCTGGA TGGGACACTG AAGCTCATGG AAGAAATGGA AGAAGCAGAC AAGCTGCTGT 961 GGTCAGTGCA GGTGGATCAC CAGCTCTTTG CCCTGGAGAA ACTGGATGTC ACCGGCAACG 1021 GGCATGAGGA GGTAGTTGCA TGCGCCTGGG ATGGACAGAC ATATATCATT GATCACAACC 1081 GCACCGTCGT CCGCTTCCAA GTGGATGAAA ATATCCGTGC CTTCTGTGCA GGCCTGTACG 1141 CCTGCAAAGA GGGCCGCAAC AGCCCCTGCC TCGTATATGT CACTTTCAAC CAGAAGATCT 1201 ATGTGTACTG GGAGGTGCAG CTGGAGCGGA TGGAGTCTAC CAATCTGGTG AAACTGCTGG 1261 AGACCAAGCC GGAGTACCAC AGCCTGCTGC AGGAGCTGGG CGTGGATCCT GACGACCTCC 1321 CTGTGACTCG TGCCCTGCTT CACCAAACGC TCTACCATCC AGACCAGCCA CCACAGTGTG 1381 CTCCCTCAAG CCTCCAGGAT CCCACCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1441 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1501 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGATTACGC 1561 AACGGCCTTG CCCTGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1621 aagatt