Transcript: Mouse XM_006505230.2

PREDICTED: Mus musculus integrin alpha FG-GAP repeat containing 2 (Itfg2), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Itfg2 (101142)
Length:
1879
CDS:
206..1009

Additional Resources:

NCBI RefSeq record:
XM_006505230.2
NBCI Gene record:
Itfg2 (101142)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006505230.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000306265 GAACGTCTCCACTCACCTAAT pLKO_005 430 CDS 100% 10.800 15.120 N Itfg2 n/a
2 TRCN0000109600 CGCAGTTATTGCAGGAATGAA pLKO.1 1395 3UTR 100% 5.625 7.875 N Itfg2 n/a
3 TRCN0000326583 CGCAGTTATTGCAGGAATGAA pLKO_005 1395 3UTR 100% 5.625 7.875 N Itfg2 n/a
4 TRCN0000109603 GCAACATCAGACAAGGTCATA pLKO.1 453 CDS 100% 4.950 3.465 N Itfg2 n/a
5 TRCN0000326516 GCAACATCAGACAAGGTCATA pLKO_005 453 CDS 100% 4.950 3.465 N Itfg2 n/a
6 TRCN0000109602 GCTTGGTATATGTCACCTTCA pLKO.1 768 CDS 100% 4.050 2.835 N Itfg2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006505230.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03665 pDONR223 100% 52.1% 52.5% None (many diffs) n/a
2 ccsbBroad304_03665 pLX_304 0% 52.1% 52.5% V5 (many diffs) n/a
3 TRCN0000472774 GATTACGCAACGGCCTTGCCCTGT pLX_317 38.4% 52.1% 52.5% V5 (many diffs) n/a
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