Construct: ORF TRCN0000472778
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008060.1_s317c1
- Derived from:
- ccsbBroadEn_03168
- DNA Barcode:
- GGTTATAGAGAGTGTTGGTATGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASCC1 (51008)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472778
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001198798.2 | 100% | 100% | |
| 2 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001198800.3 | 100% | 100% | |
| 3 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369093.1 | 100% | 100% | |
| 4 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369094.1 | 100% | 100% | |
| 5 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369095.1 | 100% | 100% | |
| 6 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369096.1 | 91.8% | 88.2% | (many diffs) |
| 7 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369097.1 | 91.8% | 88.2% | (many diffs) |
| 8 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369098.1 | 91.8% | 88.2% | (many diffs) |
| 9 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369090.1 | 91% | 88% | (many diffs) |
| 10 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369091.1 | 91% | 88% | (many diffs) |
| 11 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369092.1 | 91% | 88% | (many diffs) |
| 12 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369105.1 | 89% | 89% | 0_1ins117 |
| 13 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369106.1 | 89% | 89% | 0_1ins117 |
| 14 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369107.1 | 89% | 89% | 0_1ins117 |
| 15 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369108.1 | 88.7% | 88.5% | 626_627ins120 |
| 16 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369109.1 | 88.7% | 88.5% | 626_627ins120 |
| 17 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369087.1 | 88.2% | 85.3% | (many diffs) |
| 18 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369088.1 | 88.2% | 85.3% | (many diffs) |
| 19 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369089.1 | 88.2% | 85.3% | (many diffs) |
| 20 | human | 51008 | ASCC1 | activating signal cointegra... | XM_024448032.1 | 86.6% | 83.2% | (many diffs) |
| 21 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369086.1 | 85.9% | 83.1% | (many diffs) |
| 22 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369099.1 | 83.6% | 83.3% | 1_66del;692_693ins120 |
| 23 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369085.1 | 83.4% | 80.6% | (many diffs) |
| 24 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001198799.3 | 82.2% | 79.2% | (many diffs) |
| 25 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369110.1 | 81.5% | 81.2% | 872T>C;875_876delTTins197 |
| 26 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369111.1 | 81.5% | 81.2% | 872T>C;875_876delTTins197 |
| 27 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369101.1 | 80.5% | 77.5% | (many diffs) |
| 28 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369102.1 | 80.5% | 77.5% | (many diffs) |
| 29 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369103.1 | 80.3% | 76.9% | (many diffs) |
| 30 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369104.1 | 80.3% | 76.9% | (many diffs) |
| 31 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369100.1 | 77.7% | 74.5% | (many diffs) |
| 32 | human | 51008 | ASCC1 | activating signal cointegra... | NM_001369112.1 | 70.3% | 69.7% | 626_627ins120;752T>C;755_756delTTins197 |
| 33 | human | 51008 | ASCC1 | activating signal cointegra... | NR_045564.1 | 39.3% | 1_347del;1419_2723del | |
| 34 | mouse | 69090 | Ascc1 | activating signal cointegra... | NM_001199187.2 | 83.6% | 86.5% | (many diffs) |
| 35 | mouse | 69090 | Ascc1 | activating signal cointegra... | NM_026937.3 | 83.6% | 86.5% | (many diffs) |
| 36 | mouse | 69090 | Ascc1 | activating signal cointegra... | XM_006514040.3 | 83.6% | 86.5% | (many diffs) |
| 37 | mouse | 69090 | Ascc1 | activating signal cointegra... | XM_006514039.2 | 77.1% | 79.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1137
- ORF length:
- 1071
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agttctgcgt ccacagctta taagaattga tggccggaat tacaggaaga 121 atccagtcca agaacagacc tatcaacatg aagaagatga agaggacttc tatcaaggct 181 ccatggagtg tgctgatgag ccctgtgatg cctacgaggt ggagcagacc ccacaaggat 241 tccggtctac tttgagggcc cccagcttgc tctataagca tatagttgga aagagagggg 301 acactaggaa gaaaatagaa atggagacca aaacttctat tagcattcct aaacctggac 361 aagacgggga aattgtaatc actggccagc atcgaaatgg tgtaatttca gcccgaacac 421 ggattgatgt tcttttggac acttttcgaa gaaagcagcc cttcactcac ttccttgcct 481 ttttcctcaa tgaagttgag gttcaggaag gattcctgag attccaggag gaagtactgg 541 cgaagtgctc catggatcat ggggttgaca gcagcatttt ccagaatcct aaaaagcttc 601 atctaactat tgggatgttg gtgcttttga gtgaggaaga gatCCAGCAG ACATGTGAGA 661 TGCTACAGCA GTGTAAAGAG GAATTCATTA ATGATATTTC TGGGGGTAAA CCCCTAGAAG 721 TGGAGATGGC AGGGATAGAA TACATGAATG ATGATCCTGG CATGGTGGAT GTTCTTTACG 781 CCAAAGTCCA TATGAAAGAT GGCTCCAACA GGCTACAAGA ATTAGTTGAT CGAGTGCTGG 841 AACGTTTTCA GGCATCTGGA CTAATAGTGA AAGAGTGGAA TAGTGTGAAA CTGCATGCTA 901 CAGTTATGAA TACACTATTC AGGAAAGACC CCAATGCTGA AGGCAGGTAC AATCTCTACA 961 CAGCGGAAGG CAAATATATC TTCAAGGAAA GAGAATCATT TGATGGCCGA AATATTTTAA 1021 AGTTGTTTGA GAACTTCTAC TTTGGCTCCC TAAAGCTGAA TTCAATTCAC ATCTCTCAGA 1081 GGTTCACCGT AGACAGCTTT GGAAACTACG CTTCCTGTGG ACAAATTGAC TTCTCCTGCC 1141 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1261 ATATATCTTG TGGAAAGGAC GAGGTTATAG AGAGTGTTGG TATGTTACGC GTTAAGTCga 1321 caatcaacct ctggattaca aaatttgtga aagatt