Transcript: Human NM_001369099.1

Homo sapiens activating signal cointegrator 1 complex subunit 1 (ASCC1), transcript variant 20, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
ASCC1 (51008)
Length:
2603
CDS:
282..1301

Additional Resources:

NCBI RefSeq record:
NM_001369099.1
NBCI Gene record:
ASCC1 (51008)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001369099.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000418084 AGAATTGATGGCCGGAATTAC pLKO_005 375 CDS 100% 13.200 18.480 N ASCC1 n/a
2 TRCN0000136137 GAATCATTTGATGGCCGAAAT pLKO.1 1155 CDS 100% 10.800 7.560 N ASCC1 n/a
3 TRCN0000429066 TGCACAGTTCAGTAACGTTAA pLKO_005 1600 3UTR 100% 10.800 7.560 N ASCC1 n/a
4 TRCN0000135802 CAAGAACAGACCTATCAACAT pLKO.1 411 CDS 100% 4.950 3.465 N ASCC1 n/a
5 TRCN0000136952 CTATTGGGATGTTGGTGCTTT pLKO.1 889 CDS 100% 4.950 3.465 N ASCC1 n/a
6 TRCN0000135961 GAAAGAGTGGAATAGTGTGAA pLKO.1 1031 CDS 100% 4.950 3.465 N ASCC1 n/a
7 TRCN0000136268 GCTTCATCTAACTATTGGGAT pLKO.1 878 CDS 100% 2.640 1.848 N ASCC1 n/a
8 TRCN0000137029 CCTCAATGAAGTTGAGGTTCA pLKO.1 767 CDS 100% 0.405 0.284 N ASCC1 n/a
9 TRCN0000136016 GAAGATGAAGAGGACTTCTAT pLKO.1 435 CDS 100% 5.625 3.375 N ASCC1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001369099.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03168 pDONR223 100% 83.6% 83.3% None 1_66del;692_693ins120 n/a
2 ccsbBroad304_03168 pLX_304 0% 83.6% 83.3% V5 1_66del;692_693ins120 n/a
3 TRCN0000472778 GGTTATAGAGAGTGTTGGTATGTT pLX_317 46.1% 83.6% 83.3% V5 1_66del;692_693ins120 n/a
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